Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,194,655	T→C	E209E (GAA→GAG)	U712_10995 ←	Anthranilate synthase component 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,194,655	0	T	C	98.5%	217.3 / ‑4.8	68	E209E (GAA→GAG)	U712_10995	Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (36/31);  total (36/32)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.60e-01

CTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTG  >  CP006881/2194525‑2194783                                                                                                                                   |                                                                                                                                  cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                                                                                           >  4:46234/3‑139 (MQ=255) cctGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTAt                                                                                                                           >  4:74546/3‑139 (MQ=255)   tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCTGCAGAAAGAAAGAGCTCTTTTATGt                                                                                                                         >  2:107268/1‑139 (MQ=255)   tGGAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                                                                                         >  7:179542/1‑139 (MQ=255)     gAAGATATCGCCTGCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTTTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGt                                                                                                                         <  3:6844/137‑1 (MQ=255)           atCGCCTGGTTTTATATAGCTTTTGATGTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGGAACAGCTCTTTTTTGGTttgttgg                                                                                                                 >  6:13803/1‑138 (MQ=255)               cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                                             >  7:295377/4‑139 (MQ=255)               cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                                             >  1:47849/4‑139 (MQ=255)               cctgCTTTTATATAGCTTTTGATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCca                                                                                                             >  5:300157/4‑139 (MQ=255)                             gcttttgATTTTTTCTACATCAGCGATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                                                >  1:179382/4‑138 (MQ=255)                             gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                                                >  2:318354/4‑138 (MQ=255)                             gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                                               <  2:238672/136‑1 (MQ=255)                             gcttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                                               <  7:201918/136‑1 (MQ=255)                             gcttttgATTTTTTCTACATCAGCCATAAAAGACTATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATg                                                                                               <  7:369205/136‑1 (MQ=255)                              cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                                                >  4:248635/3‑137 (MQ=255)                              cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                                                >  3:427611/3‑137 (MQ=255)                              cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                                                                                              <  5:404018/137‑1 (MQ=255)                              cttttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATga                                                                                              >  1:248665/3‑139 (MQ=255)                                tttgATTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAAt                                                                                                >  3:392735/1‑135 (MQ=255)                                 ttgaTTTTTTCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGAt                                                                                           >  3:445617/4‑139 (MQ=255)                                       ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                                                                                      <  8:179542/138‑1 (MQ=255)                                       ttttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGa                                                                                      <  5:431863/138‑1 (MQ=255)                                        tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCTATGAGATTTTGAAg                                                                                    >  1:327908/1‑139 (MQ=255)                                        tttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg                                                                                    >  2:150761/1‑139 (MQ=255)                                         ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAg                                                                                    <  7:109156/138‑1 (MQ=255)                                         ttCTACATCAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGc                                                                                   >  8:236612/1‑139 (MQ=255)                                                 cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                                                                                                 >  5:117271/1‑115 (MQ=255)                                                 cAGCCATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTcaa                                                                                                 <  6:117271/117‑3 (MQ=255)                                                     cATAAAAGCCGATTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAACAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGAttt                                                                       <  7:222843/139‑1 (MQ=255)                                                             ccgatttttCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGgaa                                                               <  4:392735/135‑1 (MQ=255)                                                             ccgatttttCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGgaa                                                               <  1:354099/135‑1 (MQ=255)                                                                                   tACTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGTTTTTGAAGATACAGATGATTTTGATGGAATACATCCATTTTTTCTGttttt                                         >  4:267897/1‑138 (MQ=255)                                                                                    aCTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTg                                       >  4:287536/1‑139 (MQ=255)                                                                                     cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                                      <  3:65584/139‑1 (MQ=255)                                                                                     cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                                      <  2:47849/139‑1 (MQ=255)                                                                                     cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                                      <  8:295377/139‑1 (MQ=255)                                                                                      tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGtt                                     >  1:434897/1‑139 (MQ=255)                                                                                      tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGtt                                     >  5:119233/1‑139 (MQ=255)                                                                                        tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGttt                                    >  3:303000/1‑138 (MQ=255)                                                                                        tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGttt                                    <  4:354680/138‑1 (MQ=255)                                                                                            aaaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCt                                 <  3:287536/137‑1 (MQ=255)                                                                                             aaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTtga                                                                    <  5:46151/102‑1 (MQ=255)                                                                                             aaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTtga                                                                    >  6:46151/1‑102 (MQ=255)                                                                                                  gggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCATCTCCagt                          >  2:245082/1‑136 (MQ=255)                                                                                                   ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCagt                          >  8:317002/1‑135 (MQ=255)                                                                                                   ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCagt                          <  4:81931/137‑3 (MQ=255)                                                                                                   ggTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCAtt                          <  6:307581/137‑1 (MQ=255)                                                                                                      gtCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGcc                     >  4:158631/1‑139 (MQ=255)                                                                                                        cTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCtt                   >  1:105018/1‑139 (MQ=255)                                                                                                           gtatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCtt                   <  2:111982/135‑1 (MQ=255)                                                                                                           gtatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCtt                   <  6:300157/135‑1 (MQ=255)                                                                                                            tatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTT‑TGTTTCCTCTCCAGTGAGCCTTGCata              <  3:46234/139‑1 (MQ=255)                                                                                                             atGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCGTGCtta              >  6:87675/1‑139 (MQ=255)                                                                                                              tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAGTACATACATTTTTTC‑GTTTTTTGTTTACTCTCCAGTGAGCCTTGCatat             >  1:309692/1‑139 (MQ=255)                                                                                                              tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata              <  7:324871/138‑1 (MQ=255)                                                                                                              tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata              <  4:212807/138‑1 (MQ=255)                                                                                                              tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata              <  3:74546/138‑1 (MQ=255)                                                                                                              tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTATCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata              <  4:164531/138‑1 (MQ=255)                                                                                                                 aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                                      >  4:128409/1‑111 (MQ=255)                                                                                                                 aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGt                                      <  3:128409/111‑1 (MQ=255)                                                                                                                 aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTg           >  3:285953/1‑138 (MQ=255)                                                                                                                  tcatcaGAAACAAACCTCTCTTTTATGTTTTTTTCGTCCATCATTTATTCAATGATATTTTGAAGCTACAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa         <  2:327908/139‑1 (MQ=255)                                                                                                                  tCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa         <  7:366384/139‑1 (MQ=255)                                                                                                                  tCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa         <  1:150761/139‑1 (MQ=255)                                                                                                                     gcagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATa       >  1:173846/1‑138 (MQ=255)                                                                                                                      cagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATaaa     >  3:448841/1‑139 (MQ=255)                                                                                                                      cagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATaaa     <  2:248665/139‑1 (MQ=255)                                                                                                                         aaagaaaCAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTC‑GTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTg  >  5:382886/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                  CTTGGAAGATATCGCCCGCTTTTATATATTCTTTTATTTTTTCTACATCAGCCATAAAGGCTGGTTTTTCATAATTAGAAGATACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTC‑GTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTG  >  CP006881/2194525‑2194783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑