| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 2,194,744 | C→T | E180K (GAA→AAA) | U712_10995 ← | Anthranilate synthase component 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 2,194,744 | 0 | C | T | 98.5% | 201.4 / ‑4.6 | 67 | E180K (GAA→AAA) | U712_10995 | Anthranilate synthase component 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (23/42); total (23/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
ACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCG > CP006881/2194608‑2194869 | aCTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTg > 4:287536/1‑139 (MQ=255) cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGt < 8:295377/139‑1 (MQ=255) cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGt < 3:65584/139‑1 (MQ=255) cTGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGt < 2:47849/139‑1 (MQ=255) tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGtt > 1:434897/1‑139 (MQ=255) tGTCTCAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGtt > 5:119233/1‑139 (MQ=255) tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGttt > 3:303000/1‑138 (MQ=255) tctcAAAGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGttt < 4:354680/138‑1 (MQ=255) aaaGCTGGGTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCt < 3:287536/137‑1 (MQ=255) gggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCATCTCCagt > 2:245082/1‑136 (MQ=255) ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCagt < 4:81931/137‑3 (MQ=255) ggTGTCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCagt > 8:317002/1‑135 (MQ=255) ggTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTCGTTTTCTGTTTCCTGTCCAtt < 6:307581/137‑1 (MQ=255) gtCTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGcc > 4:158631/1‑139 (MQ=255) cTTGTATGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCtt > 1:105018/1‑139 (MQ=255) gtatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCtt < 2:111982/135‑1 (MQ=255) gtatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCtt < 6:300157/135‑1 (MQ=255) tatGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTT‑TGTTTCCTCTCCAGTGAGCCTTGCata < 3:46234/139‑1 (MQ=255) atGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCGTGCtta > 6:87675/1‑139 (MQ=255) tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAGTACATACATTTTTTCGTTTTTTGTTTACTCTCCAGTGAGCCTTGCatat > 1:309692/1‑139 (MQ=255) tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata < 3:74546/138‑1 (MQ=255) tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata < 4:212807/138‑1 (MQ=255) tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata < 7:324871/138‑1 (MQ=255) tGAATCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTATCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCata < 4:164531/138‑1 (MQ=255) aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGt > 4:128409/1‑111 (MQ=255) aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGt < 3:128409/111‑1 (MQ=255) aTCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTg > 3:285953/1‑138 (MQ=255) tcatcaGAAACAAACCTCTCTTTTATGTTTTTTTCGTCCATCATTTATTCAATGATATTTTGAAGCTACAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa < 2:327908/139‑1 (MQ=255) tCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa < 1:150761/139‑1 (MQ=255) tCAGCAGAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGa < 7:366384/139‑1 (MQ=255) gcagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATa > 1:173846/1‑138 (MQ=255) cagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATaaa < 2:248665/139‑1 (MQ=255) cagAAAGAAACAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATaaa > 3:448841/1‑139 (MQ=255) aaagaaaCAGCTCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTg > 5:382886/1‑139 (MQ=255) ctCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGtttttg < 4:133300/138‑2 (MQ=255) ctCTTTTATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGtttttg > 5:301578/2‑138 (MQ=255) ttATGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGttt > 3:54322/1‑138 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCa < 5:436299/138‑1 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCa < 1:245082/138‑1 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCa < 7:314623/138‑1 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCa < 2:17997/138‑1 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCa < 3:277760/138‑1 (MQ=255) aTGTTTTTTTGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTAGACGTTTTTGGTTTCa < 2:19554/138‑1 (MQ=255) ttGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATTTTGGATAAAGTGGACGTTTTTGGTTTCATGATCata < 1:14417/138‑1 (MQ=255) ttGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCata < 1:318354/138‑1 (MQ=255) ttGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCata < 4:303000/138‑1 (MQ=255) ttGGTCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCata < 2:434897/138‑1 (MQ=255) ggtCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCatacg < 4:60680/136‑3 (MQ=255) gtCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGc > 3:85370/2‑138 (MQ=255) tCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTACAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCaa < 3:267897/139‑1 (MQ=255) tttCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTcc < 5:106504/138‑1 (MQ=255) tcaaTGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCgg < 7:236612/135‑1 (MQ=255) gaTTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa < 4:427611/139‑1 (MQ=255) attttGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa < 7:317002/135‑1 (MQ=255) ttttGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCaaacaaac < 3:429691/136‑1 (MQ=255) gAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa < 2:105018/138‑1 (MQ=255) atgatTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTcc < 3:248635/139‑1 (MQ=255) atTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAt < 4:285953/138‑1 (MQ=255) ttGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc < 2:173846/138‑4 (MQ=255) ttGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc < 3:158631/138‑4 (MQ=255) aaTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCata < 4:338024/86‑1 (MQ=255) aaTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCata > 3:338024/1‑86 (MQ=255) aTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa > 7:152918/1‑114 (MQ=255) aTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa < 8:152918/114‑1 (MQ=255) tACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACCCTTTTCCATGTCTGTTTCtttg > 3:386367/1‑138 (MQ=255) ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTGATGAACATACGCAATTTAATTCCGGCAAACAAAAAACAAACACTTTTCCATGTGTGTTTATTTTGGAttgg > 4:251688/1‑135 (MQ=255) ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCg > 6:379057/1‑138 (MQ=255) | ACTGTCTCAAAGCTGGGTGTCTTATATGAATCAGCAGAAAGAAACAGTTCTTTTATGTTTTTTTGATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCG > CP006881/2194608‑2194869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |