Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,194,808 T→C A158A (GCA→GCG)  U712_10995 ← Anthranilate synthase component 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,194,8080TC100.0% 159.0 / NA 49A158A (GCA→GCG) U712_10995Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/27);  total (22/27)

ATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAA  >  CP006881/2194673‑2194935
                                                                                                                                       |                                                                                                                               
gtCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGc                                                                                                                               >  3:85370/2‑138 (MQ=255)
 tCCATCATTTTTTCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTACAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCaa                                                                                                                             <  3:267897/139‑1 (MQ=255)
           tttCAATGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTcc                                                                                                                    <  5:106504/138‑1 (MQ=255)
             tcaaTGAGATTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCgg                                                                                                                  <  7:236612/135‑1 (MQ=255)
                    gaTTTTGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa                                                                                                          <  4:427611/139‑1 (MQ=255)
                     attttGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa                                                                                                          <  7:317002/135‑1 (MQ=255)
                      ttttGAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCaaacaaac                                                                                                         <  3:429691/136‑1 (MQ=255)
                          gAAGCTCCAGATGATTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa                                                                                                     <  2:105018/138‑1 (MQ=255)
                                    atgatTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTcc                                                                                          <  3:248635/139‑1 (MQ=255)
                                       atTTTGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAt                                                                                        <  4:285953/138‑1 (MQ=255)
                                          ttGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc                                                                                     <  2:173846/138‑4 (MQ=255)
                                          ttGATGGAATACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc                                                                                     <  3:158631/138‑4 (MQ=255)
                                                  aTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa                                                                                                     <  8:152918/114‑1 (MQ=255)
                                                  aTACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa                                                                                                     >  7:152918/1‑114 (MQ=255)
                                                   tACATCCATTTTTTCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACCCTTTTCCATGTCTGTTTCtttg                                                                           >  3:386367/1‑138 (MQ=255)
                                                           ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTGATGAACATACGCAATTTAATTCCGGCAAACAAAAAACAAACACTTTTCCATGTGTGTTTATTTTGGAttgg                                                                    >  4:251688/1‑135 (MQ=255)
                                                           ttttttCGTTTTTTGTTTCCTCTCCAGTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCg                                                                    >  6:379057/1‑138 (MQ=255)
                                                                   tcttttGTATCCTCTCCAGTGAGCCTTTCATATTGGATAAAGTGGCCGAGTTTGGATTCATGATCATACCCAATTAATGTCCGGCAAACAAACCGCAAACACTTATCCATCTCTGTTTCTTTGGTAAGCGAAGGAACAg                                                           <  5:13803/134‑1 (MQ=255)
                                                                                   cagtGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                              <  2:179382/133‑1 (MQ=255)
                                                                                      tGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                         <  3:251688/138‑1 (MQ=255)
                                                                                      tGAGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                        <  6:382886/139‑1 (MQ=255)
                                                                                      tGAGCCTTGCATATTGGATAAAGTGGACGTGGTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACACCATACACTTTTCAATGTCTGTTTCTTGGGTATGCGAAGGATCACAACGCGAACTCAGCGGGAt                                        >  5:129394/1‑139 (MQ=255)
                                                                                        aGCCTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAAATAATGTCCGGCAAACAAACAGCTTACACTTTTCCATGTCTGTTTCTTTGGTTTGCGAAGGAACAGAAGGCTCAATCAGCGGTATc                                       <  5:31447/138‑1 (MQ=255)
                                                                                          ccTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAg                                                           <  6:117076/116‑1 (MQ=255)
                                                                                          ccTTGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAg                                                           >  5:117076/1‑116 (MQ=255)
                                                                                             tGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa                                                                                                          >  2:332049/1‑66 (MQ=255)
                                                                                             tGCATATTGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGcaaacaaa                                                                                                          <  1:332049/66‑1 (MQ=255)
                                                                                              gCATATTGGATAAAGTGGACGTTTTTGGTTGCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATca                                 <  1:107268/138‑2 (MQ=255)
                                                                                                   ttGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCtt                           <  5:379057/139‑1 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc                                                                                     <  2:289658/80‑4 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCAtgtc                                                                                     >  1:289658/1‑77 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                         >  6:18838/1‑124 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGa                                         <  5:18838/124‑1 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGGCGGTTTCTTTGGTATGCGTAGGAACAGAAGGCTCATTCAGCGGGATCATATCATAGGtt                           >  1:206305/1‑138 (MQ=255)
                                                                                                    tGGATAAAGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTACATGTGTGTTTCTTTGGTATGCGAATGAACAGAAGGCTCAATCAGCGGGATAATATCATAGAtt                           >  1:398069/1‑138 (MQ=255)
                                                                                                           aGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAccc                   >  3:99233/1‑139 (MQ=255)
                                                                                                           aGTGGACGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAccc                   >  5:400834/1‑139 (MQ=255)
                                                                                                                aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa                                                                                                     >  4:336526/1‑52 (MQ=255)
                                                                                                                aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCa                                                                                                     <  3:336526/52‑1 (MQ=255)
                                                                                                                aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCTGCGGGATCATATCATAGCTTAAGTACCCGACAg              >  1:402536/1‑139 (MQ=255)
                                                                                                                aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                              <  7:442972/107‑1 (MQ=255)
                                                                                                                aCGTTTTTGGTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAg                                              >  8:442972/1‑107 (MQ=255)
                                                                                                                aCGTTTTTGGGTTCATGCTCATACGCAATTAATGTGCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCTGACAg              >  4:431393/1‑139 (MQ=255)
                                                                                                                     tttGGTTTCAAGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCCTGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTAACCGACAGCTccg         >  3:317114/1‑139 (MQ=255)
                                                                                                                       tggTTTCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTccgc        <  6:119233/136‑1 (MQ=255)
                                                                                                                          tttCATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGa     >  4:168340/1‑138 (MQ=255)
                                                                                                                             cATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                        <  7:101488/100‑1 (MQ=255)
                                                                                                                             cATGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGAt                                        >  8:101488/1‑100 (MQ=255)
                                                                                                                              aTGATCATACGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACaa  >  7:206081/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                               
ATCCATCATTTTTTCTATGAGATCTTGAAGCTCTTGATGATTTTGATGAAATACATCGATTTTTTCGTTTTCTGTTTCCTGTCCATTGAGCCTTGCATATTGGATAAAGTGGACGTTTTTAGTTTCATGATCATATGCAATTAATGTCCGGCAAACAAACAGCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAA  >  CP006881/2194673‑2194935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑