Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,194,970	C→T	T104T (ACG→ACA)	U712_10995 ←	Anthranilate synthase component 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,194,970	0	C	T	100.0%	243.5 / NA	77	T104T (ACG→ACA)	U712_10995	Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/37);  total (40/37)

GCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATCCAATTCAGAACTTCTTTAAGTTCATTTCCTGTGTAATGAGATCTGCCGTCATGATCTGTAGCTGTAAAACGGCTCTGCTTTTCTTTAATCGTGAGGAATGGATTCAGGCCGATAAACG  >  CP006881/2194834‑2195100                                                                                                                                         |                                                                                                                                   gCATACACTTATCCTTGTCTGTTGCTTTGGTATGCGAATGAACAGAAGGCTCAATCAGCGGTATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGt                                                                                                                                  <  2:309692/139‑1 (MQ=255)          tttCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGCCAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCAt                                                                                                                         >  1:262393/1‑139 (MQ=255)          tttCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCAt                                                                                                                         >  7:102235/1‑139 (MQ=255)            tCCATGTCTGTTTCTTTGGTATGCGAATGAACAGATGGCTAAATCAGCGGGTTCATATCATAGCTTATGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        <  4:336903/138‑1 (MQ=255)            tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        >  1:340110/1‑138 (MQ=255)            tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        >  4:283269/1‑138 (MQ=255)            tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        >  5:28803/1‑138 (MQ=255)            tCCATGTCTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGGGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        <  4:386367/138‑1 (MQ=255)              catgtcTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCag                                                                                                                     >  1:193346/6‑138 (MQ=255)                tgtcTGTTTCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGtt                                                                                                                   >  5:363158/4‑139 (MQ=255)                 gtcTGTTTCTTTGGTATGCGAACGAACAGAAGGCTCAATCAGCGGGATCATATCATATCTTAACTACCCCACAGCTCCGCCGACAAAAGGATTGCCAAGCTCAGGTGTTTTGATTTAGTATGTGGTATTATTCCAGtt                                                                                                                   >  3:336903/3‑138 (MQ=255)                      tttCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAgca                                                                                                              <  5:87675/138‑3 (MQ=255)                      tttCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCAc                                                                                                             <  4:448841/139‑1 (MQ=255)                      tttCTTTGGTATGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCAc                                                                                                             <  4:99233/139‑1 (MQ=255)                              gtaTGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCtttta                                                                                                     >  3:318661/3‑137 (MQ=255)                              gtaTGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCtttt                                                                                                      <  3:283269/136‑2 (MQ=255)                                 tGCGAAGGAACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCACAGCTCAGGTGTTTTGATTTTGTATGTGGAATTAATCCAGTTCAGCACTTCTATTAGt                                                                                                   >  6:310360/1‑138 (MQ=255)                                    gaaggaACAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCAt                                                                                               <  5:279406/137‑1 (MQ=255)                                        gaACAGAAGGCTCAATCTGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTcc                                                                                           <  2:402536/139‑1 (MQ=255)                                        gaACAGAAGGCTCAATCAGCGGTATCTTTTCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTc                                                                                            <  2:340110/138‑1 (MQ=255)                                         aaCAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCTGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCt                                                                                          <  3:431393/139‑1 (MQ=255)                                         aaCAGAAGGCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTcc                                                                                           <  2:193346/138‑1 (MQ=255)                                         aaCAGAAGGCTCAATCAGCGGGATCATATCATAGCTAAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCt                                                                                          <  2:206305/139‑1 (MQ=255)                                              agggCTCAATCTGCGGGATCATATCATAGCTTAAGTACCCGACAGCTACGCAGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTgtgtt                                                                                     <  8:42450/137‑2 (MQ=255)                                                ggCTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaaa                                                                                   <  6:16647/139‑2 (MQ=255)                                                  cTCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaaa                                                                                   >  3:142123/1‑136 (MQ=255)                                                   tCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTc                                                                                            >  1:343684/1‑127 (MQ=255)                                                   tCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTc                                                                                            <  2:343684/127‑1 (MQ=255)                                                   tCAATCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaaa                                                                                   >  3:57529/1‑135 (MQ=255)                                                     aaTCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaaa                                                                                   <  3:77924/134‑2 (MQ=255)                                                     aaTCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaaa                                                                                   >  4:77924/1‑133 (MQ=255)                                                     aaTCAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGAt                                                                              >  7:426082/1‑139 (MQ=255)                                                        cAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaa                                                                                    <  1:348598/130‑1 (MQ=255)                                                        cAGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTaa                                                                                    >  2:348598/1‑130 (MQ=255)                                                         aGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTg                                                                           <  8:315365/138‑1 (MQ=255)                                                         aGCGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTg                                                                           <  8:102235/138‑1 (MQ=255)                                                           cGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCttt                                                                                                       >  2:172619/1‑108 (MQ=255)                                                           cGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCttt                                                                                                       <  1:172619/108‑1 (MQ=255)                                                           cGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGctg                                                                        >  7:184134/1‑137 (MQ=255)                                                           cGGGATCATATCATAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGctg                                                                        >  4:435626/1‑137 (MQ=255)                                                           cGGGATCATATCATAGCTTAAGTACCCGACACCTCCGCCGACAAAAGGAATGACAAGCTAACGTGTATTGATTTTGTATGTGGTATTCATCCAGTACAGCACTTCTTTTAGTTAAATTACTGTGTAAAGAGAttagcct                                                                        >  7:42450/1‑133 (MQ=255)                                                               atcatatcatAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTcct                                                                    >  1:437005/1‑137 (MQ=255)                                                               atcatatcatAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGAGTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTGTTAGTTCAGTTCCGGTGTAAAGAGATTTGCTGTcct                                                                    >  8:318439/1‑137 (MQ=255)                                                                     tcatAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTa                                                                                     >  5:213899/4‑116 (MQ=255)                                                                     tcatAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTa                                                                                     <  6:213899/113‑1 (MQ=255)                                                                     tcatAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATcag                                                              <  4:57529/136‑3 (MQ=255)                                                                        tAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        >  4:267448/1‑78 (MQ=255)                                                                        tAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        <  3:267448/78‑1 (MQ=255)                                                                        tAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATcagcgg                                                           >  4:124436/1‑134 (MQ=255)                                                                         aGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATcagcggc                                                          >  7:116755/1‑133 (MQ=255)                                                                           cTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCt                                                                                          <  1:337949/105‑1 (MQ=255)                                                                           cTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCt                                                                                          >  2:337949/1‑105 (MQ=255)                                                                                      gACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAagag                                                                                <  5:223784/104‑1 (MQ=255)                                                                                      gACAGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAagag                                                                                >  6:223784/1‑104 (MQ=255)                                                                                         aGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGc                                          <  6:400834/139‑1 (MQ=255)                                                                                           cTCCGCCGACAAACGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGATATTTGCTGTCCTGATCAGCGGCCTAAAAACGGCCCTGCtc                                        <  4:54322/139‑2 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        <  6:374512/59‑1 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATc                                                                                                                        >  5:374512/1‑59 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAagag                                                                                <  4:387168/99‑1 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAagag                                                                                >  3:387168/1‑99 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCTAAAAACGGCCCTGCtc                                        <  6:133882/139‑2 (MQ=255)                                                                                           cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCtc                                        >  2:353355/1‑138 (MQ=255)                                                                                              cgccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTc                                     >  8:63679/1‑139 (MQ=255)                                                                                                ccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGct                                                                         >  3:242113/1‑100 (MQ=255)                                                                                                ccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGct                                                                         <  4:242113/101‑2 (MQ=255)                                                                                                ccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTcttctt                                   >  1:44979/1‑139 (MQ=255)                                                                                                       aaGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGATATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGt                            <  3:124436/139‑1 (MQ=255)                                                                                                             tGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGaaa                      >  5:378905/1‑139 (MQ=255)                                                                                                               ccaaGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATg                    >  1:44135/4‑139 (MQ=255)                                                                                                                  aGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAACCGGCCATGCTCTTCTTTAATTGTGAGAAATGTAt                 >  4:285054/1‑139 (MQ=255)                                                                                                                  aGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGAt                 >  1:245583/1‑139 (MQ=255)                                                                                                                  aGCTCAGGTGTTTTGATTTTGTATGTGGTATTAATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGCCCCGGCTCTTCTTTAATTGTGAGAAATGGAt                 >  5:147186/1‑139 (MQ=255)                                                                                                                            gtttGATTTTGTATGGGGTACTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTGGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt       <  4:142123/138‑1 (MQ=255)                                                                                                                             tttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTATTTCTTTTCCTGTGTAAAGTGTTTTGCTGTCCTGATCAGCGGCCGAAACACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt       <  1:353355/138‑1 (MQ=255)                                                                                                                             tttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt       <  3:435626/138‑1 (MQ=255)                                                                                                                              ttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGACGa        >  5:13637/1‑136 (MQ=255)                                                                                                                                 gacaggTATGTGGTATTCATCCAGTTCAGAACTTCTTTTTGTTAATTTCCTGTGTAAAGTGATTTGCTGTCCTTATCAGCGGCCTAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACg  <  4:128686/134‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   GCATACACTTTTCCATTCCTGTTTCTTTTGAATGCGGAGGAACAGAAGGCTCAATCAGCGGGATCATATCGTAGCTTAAGTACCCGACAGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATCCAATTCAGAACTTCTTTAAGTTCATTTCCTGTGTAATGAGATCTGCCGTCATGATCTGTAGCTGTAAAACGGCTCTGCTTTTCTTTAATCGTGAGGAATGGATTCAGGCCGATAAACG  >  CP006881/2194834‑2195100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑