Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,195,045	T→C	T79S (ACA→TCG)	U712_10995 ←	Anthranilate synthase component 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,195,045	0	T	C	100.0%	176.0 / NA	56	T79S (ACA→TCG)	U712_10995	Anthranilate synthase component 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/20);  total (36/20)

AGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATCCAATTCAGAACTTCTTTAAGTTCATTTCCTGTGTAATGAGATCTGCCGTCATGATCTGTAGCTGTAAAACGGCTCTGCTTTTCTTTAATCGTGAGGAATGGATTCAGGCCGATAAACGAATATCTTGACCAATTGGATGTATCATCCTTGCTTTCAAGAAGATACGTAATCTCCCTGTCGAG  >  CP006881/2194922‑2195164                                                                                                                            |                                                                                                                        aGCTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGc                                                                                                          <  6:400834/139‑1 (MQ=255)   cTCCGCCGACAAACGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGATATTTGCTGTCCTGATCAGCGGCCTAAAAACGGCCCTGCtc                                                                                                        <  4:54322/139‑2 (MQ=255)   cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCTAAAAACGGCCCTGCtc                                                                                                        <  6:133882/139‑2 (MQ=255)   cTCCGCCGACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCtc                                                                                                        >  2:353355/1‑138 (MQ=255)      cgccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTc                                                                                                     >  8:63679/1‑139 (MQ=255)        ccgACAAAAGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTcttctt                                                                                                   >  1:44979/1‑139 (MQ=255)               aaGGAATGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGATATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGt                                                                                            <  3:124436/139‑1 (MQ=255)                     tGCCAAGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGaaa                                                                                      >  5:378905/1‑139 (MQ=255)                       ccaaGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATg                                                                                    >  1:44135/4‑139 (MQ=255)                          aGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAACCGGCCATGCTCTTCTTTAATTGTGAGAAATGTAt                                                                                 >  4:285054/1‑139 (MQ=255)                          aGCTCAGGTGTTTTGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGAt                                                                                 >  1:245583/1‑139 (MQ=255)                          aGCTCAGGTGTTTTGATTTTGTATGTGGTATTAATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGCCCCGGCTCTTCTTTAATTGTGAGAAATGGAt                                                                                 >  5:147186/1‑139 (MQ=255)                                    gtttGATTTTGTATGGGGTACTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTGGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt                                                                       <  4:142123/138‑1 (MQ=255)                                     tttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTATTTCTTTTCCTGTGTAAAGTGTTTTGCTGTCCTGATCAGCGGCCGAAACACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt                                                                       <  1:353355/138‑1 (MQ=255)                                     tttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGAt                                                                       <  3:435626/138‑1 (MQ=255)                                      ttGATTTTGTATGTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGACGa                                                                        >  5:13637/1‑136 (MQ=255)                                         gacaggTATGTGGTATTCATCCAGTTCAGAACTTCTTTTTGTTAATTTCCTGTGTAAAGTGATTTGCTGTCCTTATCAGCGGCCTAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACg                                                                  <  4:128686/134‑1 (MQ=255)                                               tatgTGGTATTCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAatat                                                             <  6:168922/135‑1 (MQ=255)                                                        ttCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCaa                                                    <  4:317114/138‑1 (MQ=255)                                                         tCATCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGAcctgtc                                                  >  3:128686/1‑135 (MQ=255)                                                           aTCCAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTgg                                                >  1:358375/1‑139 (MQ=255)                                                           aTCCAGTTCAGCACTTATTTTAGTTAATTTCCTGTTTAAATATATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTgg                                                <  6:226441/139‑1 (MQ=255)                                                             ccAGTTCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAATTGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGAt                                              >  4:425600/1‑139 (MQ=255)                                                              cagttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATg                                             <  8:35903/136‑1 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTGTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATTTCTGGACCATGTGGATGTa                                           >  4:91222/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           >  3:267514/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           >  2:116946/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           >  5:226441/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           >  5:161447/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           >  4:443346/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTa                                           <  2:44135/138‑1 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGAACAAGTGGATGTa                                           >  3:276023/2‑139 (MQ=255)                                                                gttCAGCACTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGACCTGCTCGTCTTTAATTGTGATAAATGGAGTCAGGCCGATAAACGAATATCTGGACAAAGTGGAtcga                                           >  8:52085/2‑136 (MQ=255)                                                                    agcaCTTCTTTAAGATCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGCATGTatcgc                                       >  3:342758/4‑137 (MQ=255)                                                                      caCTTCTTTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAAGTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTcc                                     >  4:398875/2‑139 (MQ=255)                                                                       aCTTTTTTTAGTTAATTTCCTTTTTAAATAGATTTGCTGTCCTGATCCGCGGCCGAAACACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCt                                    <  2:358375/139‑1 (MQ=255)                                                                          tcttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGc                                 >  1:301064/1‑139 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCaag                                                   >  2:84759/1‑119 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCaag                                                   <  1:84759/120‑2 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCt                                >  4:148806/1‑139 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCt                                >  1:323632/1‑139 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCt                                >  5:370231/1‑139 (MQ=255)                                                                           cttTTAGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCt                                >  2:6039/1‑139 (MQ=255)                                                                                aGTTCATTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTc                             >  8:390762/1‑137 (MQ=255)                                                                                aGTTCATTTCATTTGTAATGAGATTTGCTGTCCTGATCTGCGCCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTTGACCAAGTGGATGTATCGTCCTTGCTTTCa                            <  8:295499/138‑1 (MQ=255)                                                                                     aTTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCaag                          >  5:295065/1‑135 (MQ=255)                                                                                     aTTTCCTGTGTAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCaag                          >  2:407709/1‑135 (MQ=255)                                                                                            gtgtAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTg                                                 >  2:325122/1‑105 (MQ=255)                                                                                            gtgtAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTg                                                 <  1:325122/105‑1 (MQ=255)                                                                                             tgtAAAGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGAACAAGTGGATGTATCGTCCTTGCTGTCAAGAAGATACGaaa               >  3:115691/1‑138 (MQ=255)                                                                                                 aagagaTTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCAAGAAGATACGTAATCTcc          <  8:426082/137‑1 (MQ=255)                                                                                                 aTGAGATTTGCTGTCCTGATCAGCGGCCGAAAAACGGCGCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCAAGAAGATACGTAATCTcc          <  7:318439/139‑1 (MQ=255)                                                                                                  agagaTTTGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCAAGAAGATACGTAATCTccc         <  3:148806/138‑1 (MQ=255)                                                                                                       tttGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCTAGAAGATACGTAATCTCCCTGTc     >  1:228610/3‑138 (MQ=255)                                                                                                       tttGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCAAGAAGATACGTAATCTACCTGTc     >  4:140138/3‑138 (MQ=255)                                                                                                         tGCTGTCCTGATCAGCGGCCGAAAAACGGCCCTGCTCTTCTTTAATTGTGAGAAATGGATTCAGGCCGATAAACGAATATCTGGACCAAGTGGATGTATCGTCCTTGCTTTCAAGAAGATACGTAATCTCCCTGTCAAg  >  1:365240/1‑139 (MQ=255)                                                                                                                            |                                                                                                                        AGCTCCGCCGACAAAAGGAATGCCTAGCTCTGGCGTTTTGATTTTGTACGTGGTATTCATCCAATTCAGAACTTCTTTAAGTTCATTTCCTGTGTAATGAGATCTGCCGTCATGATCTGTAGCTGTAAAACGGCTCTGCTTTTCTTTAATCGTGAGGAATGGATTCAGGCCGATAAACGAATATCTTGACCAATTGGATGTATCATCCTTGCTTTCAAGAAGATACGTAATCTCCCTGTCGAG  >  CP006881/2194922‑2195164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑