Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,195,668	C→T	V71V (GTG→GTA)	U712_11000 ←	Chorismate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,195,668	0	C	T	100.0%	184.2 / NA	59	V71V (GTG→GTA)	U712_11000	Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/24);  total (35/24)

TCTTAATACAACGGCTTTTTCTAAATATACATGCCTGATCCGTTCCTGAGGAACATCTGTCTGGACGGTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAG  >  CP006881/2195533‑2195802                                                                                                                                        |                                                                                                                                       cctcaaTACGACAACTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGtaca                                                                                                                                     >  7:57/5‑139 (MQ=255)            caactatttCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATcc                                                                                                                          >  8:250709/7‑139 (MQ=255)            caacTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATcc                                                                                                                          >  5:50131/4‑139 (MQ=255)            caacTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATcc                                                                                                                          >  4:56559/4‑139 (MQ=255)               cTTTTTCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTg                                                                                                                        >  4:26857/1‑138 (MQ=255)                  tttCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATa                                                                                                                                   <  4:394660/124‑1 (MQ=255)                  tttCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATa                                                                                                                                   >  3:394660/1‑124 (MQ=255)                  tttCTAAATATACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGaaa                                                                                                                     <  7:250709/138‑1 (MQ=255)                         atatACATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTcgcga                                                                                                             <  3:26857/139‑2 (MQ=255)                          tataCATGTCTGATCTGATCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTcgcgaa                                                                                                            <  4:184988/139‑3 (MQ=255)                                    tgatctgatcCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  >  5:176726/1‑139 (MQ=255)                                    tgatctgatcCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  >  3:25913/1‑139 (MQ=255)                                    tgatctgatcCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  >  3:251046/1‑139 (MQ=255)                                    tgatctgatcCTGAGGGACATCTGTCTGGACCATCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  >  3:136726/1‑139 (MQ=255)                                      atctgatCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGcc                                                                                                 <  3:56559/132‑1 (MQ=255)                                      atctgatCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  <  5:6208/131‑1 (MQ=255)                                      atctgatCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCg                                                                                                >  6:364124/7‑139 (MQ=255)                                           atCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCCACCTGAAAGCTCTCGAACAGCTTTTGCCGGGaa                                                                                            >  7:208839/2‑138 (MQ=255)                                           atCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  <  1:12903/131‑1 (MQ=255)                                           atCCTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGaa                                                                                            >  3:374874/2‑138 (MQ=255)                                           atCCTGAGGGACATCTGTCTCGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGc                                                                                                  >  2:12903/2‑132 (MQ=255)                                              cTGAGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAAca                                                                                        <  6:50131/139‑2 (MQ=255)                                                 aGGGACATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAAcagcg                                                                                     <  4:251046/139‑5 (MQ=255)                                                   ggaCATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTg                                                                                   <  4:374874/137‑1 (MQ=255)                                                     aCATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGc                                                                                  >  4:378098/1‑138 (MQ=255)                                                      cATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAAca                                                                                        >  8:178537/1‑130 (MQ=255)                                                      cATCTGTCTGGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAAca                                                                                        <  7:178537/131‑2 (MQ=255)                                                           gtctgGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCGTGTTACGTCCATTTCCTGCATACATGTTACCGTTACATACTGCCATCCTGAAAGCACGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAg                                                                           >  2:136420/1‑139 (MQ=255)                                                              tgGACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGgtg                                                                        >  6:133972/1‑139 (MQ=255)                                                                gACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGgtgta                                                                      <  4:25913/139‑2 (MQ=255)                                                                gACCGTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGgtgta                                                                      <  4:131125/139‑2 (MQ=255)                                                                 accgTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGgtgtag                                                                     >  3:68638/4‑137 (MQ=255)                                                                    gTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGcc                                                                   >  8:6153/1‑138 (MQ=255)                                                                                 ttaTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAttt                                                      >  1:160555/3‑138 (MQ=255)                                                                                   aTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGaa                                                   >  6:250637/1‑139 (MQ=255)                                                                                         tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATTTTACAGGTACATACTGCCATCCTGAAAGCTCTCGACCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTCGCCGACAGCAGCATTTGAACAACAt                                             >  5:264582/1‑139 (MQ=255)                                                                                         tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTATTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAacaaca                                              >  6:185776/1‑138 (MQ=255)                                                                                           tttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                           >  3:362523/1‑139 (MQ=255)                                                                                           tttCAGACCGCCAGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGCACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                           >  4:340457/1‑139 (MQ=255)                                                                                                  ccgcctGTTACGTCGATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                    <  3:110466/139‑1 (MQ=255)                                                                                                  ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                    <  5:133972/139‑1 (MQ=255)                                                                                                  ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                    <  5:250637/139‑1 (MQ=255)                                                                                                     cctgTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttg                                 >  7:54440/4‑139 (MQ=255)                                                                                                         ttACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                               <  3:104516/137‑2 (MQ=255)                                                                                                                  tttCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTAGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTttcttc                    >  1:390730/1‑139 (MQ=255)                                                                                                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt             >  6:134021/1‑139 (MQ=255)                                                                                                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt             >  5:38809/1‑139 (MQ=255)                                                                                                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt             <  4:136726/139‑1 (MQ=255)                                                                                                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt             <  3:293422/139‑1 (MQ=255)                                                                                                                              atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc        >  2:105490/3‑139 (MQ=255)                                                                                                                              atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc        >  5:361422/3‑139 (MQ=255)                                                                                                                               tgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTctct       <  3:142533/138‑1 (MQ=255)                                                                                                                                 ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCTAATCAGGTGTAGCCGACAGAAGCATTTGACCAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa     >  4:59128/1‑139 (MQ=255)                                                                                                                                 ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa     <  2:160555/139‑1 (MQ=255)                                                                                                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                        >  7:86336/1‑86 (MQ=255)                                                                                                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                        <  8:86336/86‑1 (MQ=255)                                                                                                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTGATTTTCTTCTATGAtt             >  7:379941/1‑129 (MQ=255)                                                                                                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt             <  8:379941/129‑1 (MQ=255)                                                                                                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa     >  5:412547/1‑137 (MQ=255)                                                                                                                                    ccGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGACGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg  <  6:176726/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       TCTTAATACAACGGCTTTTTCTAAATATACATGCCTGATCCGTTCCTGAGGAACATCTGTCTGGACGGTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAG  >  CP006881/2195533‑2195802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑