Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,002	A→G	L308L (CTT→CTC)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,002	0	A	G	100.0%	170.8 / NA	52	L308L (CTT→CTC)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/33);  total (19/33)

TTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTC  >  CP006881/2173876‑2174137                                                                                                                               |                                                                                                                                        cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                                                                                                              <  8:392584/137‑1 (MQ=255) cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                                                                                                              <  8:182370/137‑1 (MQ=255) cttACCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                                                                                                              <  2:306096/137‑1 (MQ=255)    aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                                                                                                              >  5:62259/1‑135 (MQ=255)    aCCCGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCttt                                                                                                                              <  6:62259/135‑1 (MQ=255)     cccGTGATATGAAGATCCTTAAGGATTTTGATAGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                                                                                                         <  7:322641/139‑1 (MQ=255)     cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                                                                                                         <  5:198396/139‑1 (MQ=255)     cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                                                                                                         <  2:302637/139‑1 (MQ=255)     cccGTGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTGGCCGAAGCTGCGATATTTTGGCCGCTGAGATCAGTGCTTTTTtcc                                                                                                                         <  5:11784/139‑1 (MQ=255)     cccGTGATATCAAGATCCTTAAGGCGTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTtcc                                                                                                                         <  3:134381/139‑1 (MQ=255)         tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                     >  1:112670/1‑139 (MQ=255)         tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                     >  6:66793/1‑139 (MQ=255)         tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                     >  8:2406/1‑139 (MQ=255)         tGATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGc                                                                                                                     >  1:331649/1‑139 (MQ=255)          gATATCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                    <  5:253246/139‑1 (MQ=255)           atttCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTGAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                    <  4:422298/135‑1 (MQ=255)           atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATGGAGTTTTTCGTTTTGCCGATGCTGCGTTATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtg                                                                                                                   <  5:140245/139‑2 (MQ=255)           atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                                                                                                                   <  3:422123/139‑2 (MQ=255)           atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                    <  7:132528/138‑1 (MQ=255)           atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCt                                                                                                                    <  4:66477/138‑1 (MQ=255)           atatCAAGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAAAGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCtc                                                                                                                   <  6:204147/139‑2 (MQ=255)                aaGATCCTTAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTa                                                                                                               <  1:18396/138‑1 (MQ=255)                        tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                 <  8:209914/128‑1 (MQ=255)                        tAAGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTg                                                                                                                 >  7:209914/1‑128 (MQ=255)                         aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                      <  2:112670/138‑1 (MQ=255)                         aaGGCTTTTGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                      <  2:415330/138‑1 (MQ=255)                               tttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                >  5:393025/1‑138 (MQ=255)                                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                >  5:259403/1‑137 (MQ=255)                                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTc                                                                                                >  3:271622/1‑137 (MQ=255)                                ttGATCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCa                                                                                               <  5:66793/138‑1 (MQ=255)                                    tCGGCAGGTTTTGATAAGCGTACTGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGct                                                                                          <  4:271622/139‑1 (MQ=255)                                                      cgtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcgg                                                                         >  3:94325/6‑138 (MQ=255)                                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                       <  3:266262/135‑3 (MQ=255)                                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                       <  8:308515/135‑3 (MQ=255)                                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                       <  7:293885/135‑3 (MQ=255)                                                       gtactGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATcggca                                                                       <  5:226415/135‑3 (MQ=255)                                                           tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                             <  4:83430/97‑1 (MQ=255)                                                           tGAATGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACa                                                                                                             >  3:83430/1‑97 (MQ=255)                                                               tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                               >  5:350233/1‑139 (MQ=255)                                                               tGTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAAt                                                               >  6:290236/1‑139 (MQ=255)                                                                gTCCTTCAGTTTCTTTTCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGAGGTTTTTTCCCAGCGCTGTACAGTGTCATTGCGTCACCGCTCTGGCCAACCGTATCGGCAAGGTGAAtg                                                              >  1:215743/1‑138 (MQ=255)                                                                            cttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgat                                                   <  7:2406/134‑1 (MQ=255)                                                                             ttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgatca                                                 <  6:259403/136‑1 (MQ=255)                                                                                                 ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                   >  3:412150/1‑85 (MQ=255)                                                                                                 ttGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCa                                                                                   <  4:412150/85‑1 (MQ=255)                                                                                                  tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                      >  5:1304/1‑65 (MQ=255)                                                                                                  tGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCAt                                                                                                      <  6:1304/65‑1 (MQ=255)                                                                                                       aaGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTc                       <  2:331649/139‑1 (MQ=255)                                                                                                             cGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGaa                  >  1:359579/1‑138 (MQ=255)                                                                                                                       gCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGgcgcgt       >  1:42345/1‑137 (MQ=255)                                                                                                                           cTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCttt   <  2:42345/139‑1 (MQ=255)                                                                                                                            tGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTc  >  3:440333/1‑139 (MQ=255)                                                                                                                               |                                                                                                                                        TTTACCCGTGATATCAAGATCCCTAAGGCTTTTGATCGGCAGGTTTTGATAAGTATTTTGAATGTCCTTTAGTTTTTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTC  >  CP006881/2173876‑2174137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑