Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,195,734	C→A	A49A (GCG→GCT)	U712_11000 ←	Chorismate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,195,734	0	C	A	98.7%	242.1 / NA	78	A49A (GCG→GCT)	U712_11000	Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (45/32);  total (46/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCC  >  CP006881/2195600‑2195868                                                                                                                                       |                                                                                                                                       gTCATCATGACTCTTATGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGcc                                                                                                                                     >  8:6153/1‑138 (MQ=255)              ttaTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAttt                                                                                                                        >  1:160555/3‑138 (MQ=255)                aTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGaa                                                                                                                     >  6:250637/1‑139 (MQ=255)                      tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATTTTACAGGTACATACTGCCATCCTGAAAGCTCTCGACCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTCGCCGACAGCAGCATTTGAACAACAt                                                                                                               >  5:264582/1‑139 (MQ=255)                      tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTATTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAacaaca                                                                                                                >  6:185776/1‑138 (MQ=255)                        tttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                                                                                             >  3:362523/1‑139 (MQ=255)                        tttCAGACCGCCAGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGCACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                                                                                             >  4:340457/1‑139 (MQ=255)                               ccgcctGTTACGTCGATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                      <  3:110466/139‑1 (MQ=255)                               ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                      <  5:250637/139‑1 (MQ=255)                               ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                      <  5:133972/139‑1 (MQ=255)                                  cctgTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttg                                                                                                   >  7:54440/4‑139 (MQ=255)                                      ttACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                 <  3:104516/137‑2 (MQ=255)                                               tttCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTAGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTttcttc                                                                                      >  1:390730/1‑139 (MQ=255)                                                      cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               >  5:38809/1‑139 (MQ=255)                                                      cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               >  6:134021/1‑139 (MQ=255)                                                      cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               <  4:136726/139‑1 (MQ=255)                                                      cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               <  3:293422/139‑1 (MQ=255)                                                           atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc                                                                          >  5:361422/3‑139 (MQ=255)                                                           atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc                                                                          >  2:105490/3‑139 (MQ=255)                                                            tgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTctct                                                                         <  3:142533/138‑1 (MQ=255)                                                              ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCTAATCAGGTGTAGCCGACAGAAGCATTTGACCAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                       >  4:59128/1‑139 (MQ=255)                                                              ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                       <  2:160555/139‑1 (MQ=255)                                                                aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                          <  8:86336/86‑1 (MQ=255)                                                                aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                          >  7:86336/1‑86 (MQ=255)                                                                aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTGATTTTCTTCTATGAtt                                                                               >  7:379941/1‑129 (MQ=255)                                                                aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               <  8:379941/129‑1 (MQ=255)                                                                aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                       >  5:412547/1‑137 (MQ=255)                                                                 ccGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGACGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                    <  6:176726/139‑1 (MQ=255)                                                                     tacaTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgt                                                                >  5:317818/2‑139 (MQ=255)                                                                     tacaTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGAGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgt                                                                >  7:193071/2‑139 (MQ=255)                                                                        aTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgttta                                                             >  1:80970/1‑138 (MQ=255)                                                                          aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTc                                                                                                           <  1:394234/91‑1 (MQ=255)                                                                          aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTc                                                                                                           >  2:394234/1‑91 (MQ=255)                                                                          aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                    <  5:298269/130‑1 (MQ=255)                                                                          aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTGCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                    >  6:298269/1‑130 (MQ=255)                                                                            tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                         >  4:218253/1‑139 (MQ=255)                                                                            tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                         >  6:174154/1‑139 (MQ=255)                                                                            tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                         >  2:258661/1‑139 (MQ=255)                                                                              ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                       >  1:215938/1‑139 (MQ=255)                                                                              ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                       >  7:231128/1‑139 (MQ=255)                                                                              ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                       >  7:252753/1‑139 (MQ=255)                                                                              ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                       >  4:218449/1‑139 (MQ=255)                                                                               cATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGtttttt                                                      >  1:381114/1‑139 (MQ=255)                                                                                 tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                     >  7:419433/1‑138 (MQ=255)                                                                                 tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                     >  1:362586/1‑138 (MQ=255)                                                                                 tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                     >  4:116937/1‑138 (MQ=255)                                                                                  ccTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                          >  1:393660/1‑68 (MQ=255)                                                                                  ccTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                          <  2:393660/68‑1 (MQ=255)                                                                                      aaaGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTaaa                                                 <  3:116937/137‑1 (MQ=255)                                                                                        aGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               >  3:190632/1‑105 (MQ=255)                                                                                        aGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                               <  4:190632/105‑1 (MQ=255)                                                                                         gCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttc                                            <  3:378098/139‑1 (MQ=255)                                                                                         gCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttc                                            <  2:80970/139‑1 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTATAATttcttct                                        >  3:382138/5‑139 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                        >  7:332636/5‑139 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                        >  2:106717/5‑139 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                          <  6:361422/133‑1 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                          <  4:362523/133‑1 (MQ=255)                                                                                             gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCAGACAGAAGCATTTGAACATCATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                          <  1:136420/133‑1 (MQ=255)                                                                                              cgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                        >  8:4786/4‑138 (MQ=255)                                                                                                     cTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtc                                <  3:218449/139‑1 (MQ=255)                                                                                                     cTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtc                                >  4:37295/1‑139 (MQ=255)                                                                                                      gtttGCCGGGAAACCAGCGTGCAATTCAGGGGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTGTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtcc                               <  8:208839/138‑2 (MQ=255)                                                                                                         tGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCg                             <  5:185776/138‑1 (MQ=255)                                                                                                           ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGtt                           <  8:252753/138‑1 (MQ=255)                                                                                                           ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                          <  8:332636/139‑1 (MQ=255)                                                                                                           ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                          <  2:390730/139‑1 (MQ=255)                                                                                                           ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                          <  7:6153/139‑1 (MQ=255)                                                                                                              ggAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAAc                       <  2:381114/139‑1 (MQ=255)                                                                                                               gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                      >  7:23718/1‑139 (MQ=255)                                                                                                               gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                      >  6:164023/1‑139 (MQ=255)                                                                                                                   acagcgTGAAAATCAGGTTTAGCCGACAGAAGCATTTTAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACATCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                  <  4:382138/134‑1 (MQ=255)                                                                                                                       cgTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTg               >  4:372963/2‑138 (MQ=255)                                                                                                                         tGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                 >  7:321110/1‑53 (MQ=255)                                                                                                                         tGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                 <  8:321110/54‑2 (MQ=255)                                                                                                                                   gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                  <  4:90606/123‑1 (MQ=255)                                                                                                                                   gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                  >  3:90606/1‑123 (MQ=255)                                                                                                                                   gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattcc  >  7:308017/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                       GTCATCATGACTCTGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCC  >  CP006881/2195600‑2195868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑