Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,195,740	A→C	L47L (CTT→CTG)	U712_11000 ←	Chorismate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,195,740	0	A	C	98.9%	297.0 / NA	91	L47L (CTT→CTG)	U712_11000	Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (51/39);  total (51/40)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAA  >  CP006881/2195613‑2195873                                                                                                                                |                                                                                                                                      ttaTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAttt                                                                                                                             >  1:160555/3‑138 (MQ=255)   aTGCACTTTTTCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGaa                                                                                                                          >  6:250637/1‑139 (MQ=255)         tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATTTTACAGGTACATACTGCCATCCTGAAAGCTCTCGACCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTCGCCGACAGCAGCATTTGAACAACAt                                                                                                                    >  5:264582/1‑139 (MQ=255)         tttttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTATTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAacaaca                                                                                                                     >  6:185776/1‑138 (MQ=255)           tttCAGACCGCCTGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                                                                                                  >  3:362523/1‑139 (MQ=255)           tttCAGACCGCCAGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGCACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCt                                                                                                                  >  4:340457/1‑139 (MQ=255)                  ccgcctGTTACGTCGATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                           <  3:110466/139‑1 (MQ=255)                  ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                           <  5:250637/139‑1 (MQ=255)                  ccgcctGTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgtt                                                                                                           <  5:133972/139‑1 (MQ=255)                     cctgTTACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttg                                                                                                        >  7:54440/4‑139 (MQ=255)                         ttACGTCCATTTCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                      <  3:104516/137‑2 (MQ=255)                                  tttCCTGCATACATGTTACCGGTACATACTGCCATCCTGAAAGCTAGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTttcttc                                                                                           >  1:390730/1‑139 (MQ=255)                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    >  6:134021/1‑139 (MQ=255)                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    >  5:38809/1‑139 (MQ=255)                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    <  3:293422/139‑1 (MQ=255)                                         cATACATGTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    <  4:136726/139‑1 (MQ=255)                                              atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc                                                                               >  5:361422/3‑139 (MQ=255)                                              atgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTtctc                                                                               >  2:105490/3‑139 (MQ=255)                                               tgTTACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTctct                                                                              <  3:142533/138‑1 (MQ=255)                                                 ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCTAATCAGGTGTAGCCGACAGAAGCATTTGACCAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                            >  4:59128/1‑139 (MQ=255)                                                 ttACCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                            <  2:160555/139‑1 (MQ=255)                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                               <  8:86336/86‑1 (MQ=255)                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                               >  7:86336/1‑86 (MQ=255)                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTGATTTTCTTCTATGAtt                                                                                    >  7:379941/1‑129 (MQ=255)                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    <  8:379941/129‑1 (MQ=255)                                                   aCCGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTaa                                                                            >  5:412547/1‑137 (MQ=255)                                                    ccGGTACATACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGACGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                         <  6:176726/139‑1 (MQ=255)                                                        tacaTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgt                                                                     >  5:317818/2‑139 (MQ=255)                                                        tacaTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGAGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgt                                                                     >  7:193071/2‑139 (MQ=255)                                                           aTACTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgttta                                                                  >  1:80970/1‑138 (MQ=255)                                                             aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTc                                                                                                                >  2:394234/1‑91 (MQ=255)                                                             aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTc                                                                                                                <  1:394234/91‑1 (MQ=255)                                                             aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                         <  5:298269/130‑1 (MQ=255)                                                             aCTGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTGCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAg                                                                         >  6:298269/1‑130 (MQ=255)                                                               tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                              >  4:218253/1‑139 (MQ=255)                                                               tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                              >  2:258661/1‑139 (MQ=255)                                                               tGCCATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCtgtttagttt                                                              >  6:174154/1‑139 (MQ=255)                                                                 ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                            >  4:218449/1‑139 (MQ=255)                                                                 ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                            >  7:231128/1‑139 (MQ=255)                                                                 ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                            >  7:252753/1‑139 (MQ=255)                                                                 ccATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGttttt                                                            >  1:215938/1‑139 (MQ=255)                                                                  cATCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGtttttt                                                           >  1:381114/1‑139 (MQ=255)                                                                    tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                          >  1:362586/1‑138 (MQ=255)                                                                    tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                          >  4:116937/1‑138 (MQ=255)                                                                    tCCTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTg                                                          >  7:419433/1‑138 (MQ=255)                                                                     ccTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                               <  2:393660/68‑1 (MQ=255)                                                                     ccTGAAAGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCAt                                                                                                                               >  1:393660/1‑68 (MQ=255)                                                                         aaaGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTaaa                                                      <  3:116937/137‑1 (MQ=255)                                                                           aGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    >  3:190632/1‑105 (MQ=255)                                                                           aGCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGAtt                                                                                    <  4:190632/105‑1 (MQ=255)                                                                            gCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttc                                                 <  2:80970/139‑1 (MQ=255)                                                                            gCTCGCGAACAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttc                                                 <  3:378098/139‑1 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTATAATttcttct                                             >  3:382138/5‑139 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                             >  7:332636/5‑139 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                             >  2:106717/5‑139 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                               <  6:361422/133‑1 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                               <  4:362523/133‑1 (MQ=255)                                                                                gcgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCAGACAGAAGCATTTGAACATCATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttctt                                               <  1:136420/133‑1 (MQ=255)                                                                                 cgaaCAGCTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATttcttct                                             >  8:4786/4‑138 (MQ=255)                                                                                        cTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtc                                     >  4:37295/1‑139 (MQ=255)                                                                                        cTTTTGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtc                                     <  3:218449/139‑1 (MQ=255)                                                                                         gtttGCCGGGAAACCAGCGTGCAATTCAGGGGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTGTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTAtcc                                    <  8:208839/138‑2 (MQ=255)                                                                                            tGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCg                                  <  5:185776/138‑1 (MQ=255)                                                                                              ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGtt                                <  8:252753/138‑1 (MQ=255)                                                                                              ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                               <  2:390730/139‑1 (MQ=255)                                                                                              ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                               <  8:332636/139‑1 (MQ=255)                                                                                              ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                               <  7:6153/139‑1 (MQ=255)                                                                                                 ggAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAAc                            <  2:381114/139‑1 (MQ=255)                                                                                                  gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                           >  6:164023/1‑139 (MQ=255)                                                                                                  gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                           >  7:23718/1‑139 (MQ=255)                                                                                                      acagcgTGAAAATCAGGTTTAGCCGACAGAAGCATTTTAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACATCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                       <  4:382138/134‑1 (MQ=255)                                                                                                          cgTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTg                    >  4:372963/2‑138 (MQ=255)                                                                                                            tGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                      >  7:321110/1‑53 (MQ=255)                                                                                                            tGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGgttttgta                                                                                                      <  8:321110/54‑2 (MQ=255)                                                                                                                      gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                       <  4:90606/123‑1 (MQ=255)                                                                                                                      gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                       >  3:90606/1‑123 (MQ=255)                                                                                                                      gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattcc       >  7:308017/1‑139 (MQ=255)                                                                                                                        gtagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                       >  3:298885/4‑121 (MQ=255)                                                                                                                        gtagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                       <  4:298885/118‑1 (MQ=255)                                                                                                                         tagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACATCTGTTTAGTTTTTTTTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgagaattcagc     >  7:415987/3‑138 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  >  2:235658/1‑139 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  5:164023/139‑1 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  5:364124/139‑1 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  >  6:414423/1‑139 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  >  7:223007/1‑139 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  >  4:99286/1‑139 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  5:134021/139‑1 (MQ=255)                                                                                                                           gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  1:258661/139‑1 (MQ=255)                                                                                                                           gCCGACAGAAGCATGTGAACAACATCTTCCGGTTTTGTATGATTTGCTTCTCTGATCTGCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccccgaaatccgcgaa  <  8:103475/139‑1 (MQ=255)                                                                                                                           gCCGAAAGAAGCATATGAACAACATCATCCGGGTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGATGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  8:193071/139‑1 (MQ=255)                                                                                                                            ccGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTTAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa  <  3:372963/138‑1 (MQ=255)                                                                                                                                |                                                                                                                                      TGATGCACTTTTTCAGACCGCCGGTTACGTCCATTTCCTGCATACAGGTTACCGGCACATACTGCCATCCTGAAAGCTCACGGACAGCTTTTGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAA  >  CP006881/2195613‑2195873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑