Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,195,839	T→C	R14R (CGA→CGG)	U712_11000 ←	Chorismate mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,195,839	0	T	C	100.0%	204.9 / NA	63	R14R (CGA→CGG)	U712_11000	Chorismate mutase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/37);  total (26/37)

TGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGC  >  CP006881/2195704‑2195970                                                                                                                                        |                                                                                                                                    tGCCGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCg                                                                                                                                   <  5:185776/138‑1 (MQ=255)   ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGtt                                                                                                                                 <  8:252753/138‑1 (MQ=255)   ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                                                                                                                                <  8:332636/139‑1 (MQ=255)   ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                                                                                                                                <  7:6153/139‑1 (MQ=255)   ccGGGAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                                                                                                                                <  2:390730/139‑1 (MQ=255)      ggAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAAc                                                                                                                             <  2:381114/139‑1 (MQ=255)       gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                                                                                                                            >  7:23718/1‑139 (MQ=255)       gAAAACAGCGTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACt                                                                                                                            >  6:164023/1‑139 (MQ=255)           acagcgTGAAAATCAGGTTTAGCCGACAGAAGCATTTTAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACATCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                                                                                                                        <  4:382138/134‑1 (MQ=255)               cgTGCAAATCAGGTGTAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTg                                                                                                                     >  4:372963/2‑138 (MQ=255)                           gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                                                                                                                        <  4:90606/123‑1 (MQ=255)                           gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                                                                                                                        >  3:90606/1‑123 (MQ=255)                           gtgtAGCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattcc                                                                                                        >  7:308017/1‑139 (MQ=255)                             gtagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                                                                                                                        >  3:298885/4‑121 (MQ=255)                             gtagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAg                                                                                                                        <  4:298885/118‑1 (MQ=255)                              tagCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACATCTGTTTAGTTTTTTTTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgagaattcagc                                                                                                      >  7:415987/3‑138 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   >  4:99286/1‑139 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  5:134021/139‑1 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  5:164023/139‑1 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  1:258661/139‑1 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  5:364124/139‑1 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   >  6:414423/1‑139 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   >  7:223007/1‑139 (MQ=255)                                gCCGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   >  2:235658/1‑139 (MQ=255)                                gCCGACAGAAGCATGTGAACAACATCTTCCGGTTTTGTATGATTTGCTTCTCTGATCTGCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccccgaaatccgcgaa                                                                                                   <  8:103475/139‑1 (MQ=255)                                gCCGAAAGAAGCATATGAACAACATCATCCGGGTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGATGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  8:193071/139‑1 (MQ=255)                                 ccGACAGAAGCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTTAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  3:372963/138‑1 (MQ=255)                                   gacagaagCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAatca                                                                                                <  3:218253/136‑1 (MQ=255)                                     cagaagCATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAatcat                                                                                               >  2:87874/2‑138 (MQ=255)                                           cATTTGAACAACATCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCATCTTCAGTATCCCGTTCAACTGTAGTTGCTCAGCGAATACAGCGAATCATCaagatc                                                                                        >  6:431359/1‑138 (MQ=255)                                                      caTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                                                                                                                                >  8:79379/1‑88 (MQ=255)                                                      caTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTc                                                                                                                                <  7:79379/88‑1 (MQ=255)                                                      caTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   >  8:289123/1‑117 (MQ=255)                                                      caTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCtccgcgaattccgcgaa                                                                                                   <  7:289123/117‑1 (MQ=255)                                                      caTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAAt                                                                             >  4:366308/1‑139 (MQ=255)                                                       aTCTTCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATc                                                                            <  3:37295/139‑1 (MQ=255)                                                           tCCGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCa                                                                        >  7:29015/1‑139 (MQ=255)                                                             cGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCAt                                                                       <  2:362586/138‑1 (MQ=255)                                                             cGGTTTTGTATGATTTTCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCAt                                                                       <  6:390229/138‑1 (MQ=255)                                                                ttttgtatgattttCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGt                                                                   >  1:302167/1‑139 (MQ=255)                                                                         attttCTTCTATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTcagccag                                                           <  7:4786/137‑1 (MQ=255)                                                                             tcttctATGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGctct                                                       >  5:35343/1‑138 (MQ=255)                                                                                    tGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGtt                                                 <  5:414423/137‑1 (MQ=255)                                                                                    tGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGtt                                                 <  3:99286/137‑1 (MQ=255)                                                                                    tGATTTTCTCTAACAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGtg                                                 <  8:54440/137‑2 (MQ=255)                                                                                                aCAGCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGaa                                      <  3:59128/136‑1 (MQ=255)                                                                                                   gCTGTTTAGTTTTTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATgta                                <  8:308017/139‑3 (MQ=255)                                                                                                    cTGTTTAGTTTTTTGTAAAATTTCTTCTTCATTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTGCAGCCAGCTCTCAAGTTCATATCTGCAAAAttaat                               >  4:19893/1‑135 (MQ=255)                                                                                                      gtttagttttTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGctc                                                        >  4:2326/1‑112 (MQ=255)                                                                                                      gtttagttttTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGctc                                                        <  3:2326/112‑1 (MQ=255)                                                                                                           gttttTTGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAg                         <  5:228994/138‑1 (MQ=255)                                                                                                               tttGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACt                    >  4:410221/1‑139 (MQ=255)                                                                                                                ttGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACt                    <  1:105490/138‑1 (MQ=255)                                                                                                                 tGTAAAATTTCTTCTTCAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACttt                  >  3:103969/1‑139 (MQ=255)                                                                                                                 tGTAAAATTTCTTCTTCAGTATCCCGTTAAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACttt                  >  5:410145/1‑139 (MQ=255)                                                                                                                                 cAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCGAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAg   <  1:106717/138‑1 (MQ=255)                                                                                                                                 cAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGa    <  1:87874/137‑1 (MQ=255)                                                                                                                                 cAGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGa    <  1:235658/137‑1 (MQ=255)                                                                                                                                  aGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGc  <  3:284699/138‑1 (MQ=255)                                                                                                                                  aGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGc  >  8:246428/1‑138 (MQ=255)                                                                                                                                  aGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGc  >  4:284699/1‑138 (MQ=255)                                                                                                                                  aGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGc  <  7:246428/138‑1 (MQ=255)                                                                                                                                  aGTATCCCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGc  <  3:366308/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    TGCCGGGAAAACGGAGTGCAAATCAGGTGTCGCCGAAAGAAGCATTTGAACAACATCTTCCGGTTTTGTTTTGTTTTCTTCTATGATTTTCTCTAACAGCTGTTTTGTTTTTTGTAAAATTTCTTCTTCAGTATCTCGTTCAACTGTAGTTGCTCCGCGAATTCCGCGAATCATCATGATGTCTCCTCCAATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGC  >  CP006881/2195704‑2195970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑