Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,196,027	T→G	L313L (CTA→CTC)	U712_11005 ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,196,027	0	T	G	100.0%	220.6 / NA	68	L313L (CTA→CTC)	U712_11005	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (35/33);  total (35/33)

ATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCG  >  CP006881/2195894‑2196159                                                                                                                                      |                                                                                                                                     aTCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCACGAAAAAGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAAACAACACGCCACCGGAGTTCTAATCATTAATCATACGGTTCAAGAGAAc                                                                                                                                 >  2:135035/1‑139 (MQ=255)  tCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAAcc                                                                                                                                >  3:360337/1‑139 (MQ=255)      cATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAACCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAg                                                                                                                            >  7:2848/1‑139 (MQ=255)           gTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTc                                                                                                                        <  4:103969/138‑1 (MQ=255)            ttCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCg                                                                                                                      <  6:38809/139‑1 (MQ=255)              cagccagcTATCAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCAGAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGt                                                                                                                     <  8:23718/138‑1 (MQ=255)                         cAAGTTCATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCttttctgat                                                                                                         <  4:28854/139‑6 (MQ=255)                               cATTTCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGAc                                                                                                    <  8:37723/138‑1 (MQ=255)                                  ttCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTgg                                                                                                >  7:239548/1‑139 (MQ=255)                                  ttCTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTg                                                                                                 >  7:176453/1‑138 (MQ=255)                                    cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGTGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa                                                                                               <  6:410145/138‑1 (MQ=255)                                    cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa                                                                                               <  6:84581/138‑1 (MQ=255)                                    cTGGAAAATGTATGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGa                                                                                               <  4:360337/138‑1 (MQ=255)                                           atgtatgatgaGCAACTTTCGCTAATTCTGTGAGCACATTAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCAGCATACGGTTCAAGAGAAGCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATATCCCAg                                                                                       <  2:313797/134‑1 (MQ=255)                                           atgtatGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCCTCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg                                                                                       <  3:19893/134‑1 (MQ=255)                                           atgtatGATAAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGATCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg                                                                                       <  5:431359/134‑1 (MQ=255)                                            tgtatGATCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAgg                                                                                      >  8:28423/5‑139 (MQ=255)                                                   tCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCttttta                                                                               <  8:2848/139‑1 (MQ=255)                                                       cAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTAGCCa                                                                           >  3:53272/1‑139 (MQ=255)                                                                   aTTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAAc                                                                <  7:34876/138‑1 (MQ=255)                                                                    ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt                                                              >  7:375647/1‑139 (MQ=255)                                                                    ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACGTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt                                                              >  6:900/1‑139 (MQ=255)                                                                         tGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt                                                         >  8:429580/1‑139 (MQ=255)                                                                          gAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt                                                         <  3:60894/138‑1 (MQ=255)                                                                             cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGAATTGGATAACACAGGCGTTTTAGCCAGCTGACCAAACGTTTTCTGTc                                                      >  4:89703/1‑138 (MQ=255)                                                                             cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCCGGTTTCGGATTTGACATGGATAACCCCGGCGTTTTAGGCAGCTGACCAAACGTTTTATGTc                                                      >  6:66415/1‑138 (MQ=255)                                                                                  tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt                                                           >  5:204472/1‑127 (MQ=255)                                                                                  tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt                                                           <  6:204472/128‑2 (MQ=255)                                                                                    aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTcac                                              <  6:410882/139‑1 (MQ=255)                                                                                    aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               <  8:239548/138‑1 (MQ=255)                                                                                     aCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               >  7:56049/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTGTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGCCCAAACGTTTTCTGTCCATTTCACAGccta                                        >  7:417312/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg                                                                                       >  8:148010/1‑92 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAg                                                                                       <  7:148010/92‑1 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTTTCCATTTCACAGccta                                        >  2:181963/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                        >  8:26394/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                        >  4:93545/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                        >  8:368071/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccaa                                        >  2:231931/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTCGCCAGATGCCCAAACGTTTTCTGTCCATTTCACAGcctc                                        >  4:198445/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTGTTCTGATTTTAGTTGGATTACCCCGGCTTTTTTGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGccta                                        >  1:143465/1‑137 (MQ=255)                                                                                          aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTACGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAggcta                                        <  1:135035/139‑5 (MQ=255)                                                                                              tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt                                    >  3:129595/1‑133 (MQ=255)                                                                                              tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt                                    >  7:65395/1‑133 (MQ=255)                                                                                               cccGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCCAACGTTTTCTGTCCATTTCACAGcctac                                       <  6:343478/135‑4 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  1:224719/136‑1 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  4:53272/136‑1 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  6:252034/136‑1 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  4:153830/136‑1 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  5:174154/136‑1 (MQ=255)                                                                                                   ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  8:176453/136‑1 (MQ=255)                                                                                                         gTTTTCTTATCATTCATCATACGGTGCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCtt                          <  5:423230/138‑1 (MQ=255)                                                                                                            ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               >  4:363709/1‑114 (MQ=255)                                                                                                            ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               <  3:363709/114‑1 (MQ=255)                                                                                                            ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGCCTACACTCTTTTAGCTtata                       >  4:2036/1‑138 (MQ=255)                                                                                                                 aTCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTtatatat                    <  4:395418/136‑1 (MQ=255)                                                                                                                   cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               >  3:397441/1‑107 (MQ=255)                                                                                                                   cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                               <  4:397441/107‑1 (MQ=255)                                                                                                                   cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTAAATATAAAg                >  7:7070/1‑138 (MQ=255)                                                                                                                   cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCGCTTTTATGATTTGACTTGGATAACCCACGCTTTTTAGCCAGCGGACCAAACGTTTTCTGTCCATTTGACATCCTACAGTCTTTTAGCGTTTATATaaat                >  2:195551/1‑137 (MQ=255)                                                                                                                    attcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGc               >  1:43975/1‑138 (MQ=255)                                                                                                                        atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc                                                                     <  7:407578/80‑1 (MQ=255)                                                                                                                        atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc                                                                     >  8:407578/1‑80 (MQ=255)                                                                                                                             aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               <  3:111418/113‑1 (MQ=255)                                                                                                                             aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                               >  4:111418/1‑113 (MQ=255)                                                                                                                                gttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCg  <  3:410221/136‑1 (MQ=255)                                                                                                                                 ttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc   >  7:375832/3‑137 (MQ=255)                                                                                                                                 ttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc   >  8:357222/3‑137 (MQ=255)                                                                                                                                      |                                                                                                                                     ATCGCCATTTGTTCAGCCAGCTCTCAAGTTCATTTCTGGAAAATGCGTGATCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCG  >  CP006881/2195894‑2196159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑