Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,196,079	A→T	I296K (ATA→AAA)	U712_11005 ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,196,079	0	A	T	100.0%	269.5 / NA	85	I296K (ATA→AAA)	U712_11005	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (48/37);  total (48/37)

TCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACG  >  CP006881/2195944‑2196213                                                                                                                                        |                                                                                                                                       tCAGCAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCttttta                                                                                                                                     <  8:2848/139‑1 (MQ=255)     cAACTTTCCCTAATTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTAGCCa                                                                                                                                 >  3:53272/1‑139 (MQ=255)                 aTTCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAAc                                                                                                                      <  7:34876/138‑1 (MQ=255)                  ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt                                                                                                                    >  7:375647/1‑139 (MQ=255)                  ttCGTTGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACGTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGt                                                                                                                    >  6:900/1‑139 (MQ=255)                       tGAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt                                                                                                               >  8:429580/1‑139 (MQ=255)                        gAGCACAATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCt                                                                                                               <  3:60894/138‑1 (MQ=255)                           cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGAATTGGATAACACAGGCGTTTTAGCCAGCTGACCAAACGTTTTCTGTc                                                                                                            >  4:89703/1‑138 (MQ=255)                           cacaATAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCCGGTTTCGGATTTGACATGGATAACCCCGGCGTTTTAGGCAGCTGACCAAACGTTTTATGTc                                                                                                            >  6:66415/1‑138 (MQ=255)                                tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt                                                                                                                 <  6:204472/128‑2 (MQ=255)                                tAAACTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGtttt                                                                                                                 >  5:204472/1‑127 (MQ=255)                                  aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTcac                                                                                                    <  6:410882/139‑1 (MQ=255)                                  aaCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     <  8:239548/138‑1 (MQ=255)                                   aCTGAATCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     >  7:56049/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTGTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGCCCAAACGTTTTCTGTCCATTTCACAGccta                                                                                              >  7:417312/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTTTCCATTTCACAGccta                                                                                              >  2:181963/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                                                                              >  8:26394/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                                                                              >  8:368071/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccta                                                                                              >  4:93545/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGccaa                                                                                              >  2:231931/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTCGCCAGATGCCCAAACGTTTTCTGTCCATTTCACAGcctc                                                                                              >  4:198445/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTGTTCTGATTTTAGTTGGATTACCCCGGCTTTTTTGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGccta                                                                                              >  1:143465/1‑137 (MQ=255)                                        aTCTTCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTACGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAggcta                                                                                              <  1:135035/139‑5 (MQ=255)                                            tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt                                                                                          >  3:129595/1‑133 (MQ=255)                                            tCCCGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGcctacagt                                                                                          >  7:65395/1‑133 (MQ=255)                                             cccGCCACGGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCCAACGTTTTCTGTCCATTTCACAGcctac                                                                                             <  6:343478/135‑4 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  6:252034/136‑1 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  5:174154/136‑1 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  1:224719/136‑1 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  4:53272/136‑1 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  4:153830/136‑1 (MQ=255)                                                 ccacgGGTTTTCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  8:176453/136‑1 (MQ=255)                                                       gTTTTCTTATCATTCATCATACGGTGCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCtt                                                                                <  5:423230/138‑1 (MQ=255)                                                          ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     <  3:363709/114‑1 (MQ=255)                                                          ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     >  4:363709/1‑114 (MQ=255)                                                          ttCTTATCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCGTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGCCTACACTCTTTTAGCTtata                                                                             >  4:2036/1‑138 (MQ=255)                                                               aTCATTCATCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTtatatat                                                                          <  4:395418/136‑1 (MQ=255)                                                                 cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     <  4:397441/107‑1 (MQ=255)                                                                 cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTca                                                                                                     >  3:397441/1‑107 (MQ=255)                                                                 cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTAAATATAAAg                                                                      >  7:7070/1‑138 (MQ=255)                                                                 cattcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCGCTTTTATGATTTGACTTGGATAACCCACGCTTTTTAGCCAGCGGACCAAACGTTTTCTGTCCATTTGACATCCTACAGTCTTTTAGCGTTTATATaaat                                                                      >  2:195551/1‑137 (MQ=255)                                                                  attcatCATACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGc                                                                     >  1:43975/1‑138 (MQ=255)                                                                      atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc                                                                                                                           >  8:407578/1‑80 (MQ=255)                                                                      atcatACGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGAc                                                                                                                           <  7:407578/80‑1 (MQ=255)                                                                           aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     >  4:111418/1‑113 (MQ=255)                                                                           aCGGTTCAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCtttt                                                                                     <  3:111418/113‑1 (MQ=255)                                                                              gttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCg                                                        <  3:410221/136‑1 (MQ=255)                                                                               ttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc                                                         >  8:357222/3‑137 (MQ=255)                                                                               ttcAAGAGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc                                                         >  7:375832/3‑137 (MQ=255)                                                                                    gaGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGc                                                   >  3:247966/2‑138 (MQ=255)                                                                                    gaGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCAATCCGAGGGc                                                   >  7:145441/2‑138 (MQ=255)                                                                                     aGAACCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTa                                                 <  1:181963/139‑1 (MQ=255)                                                                                       aaCCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACg                                               >  5:346958/1‑139 (MQ=255)                                                                                       aaCCGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCGTTTTAGCCCGCTGCCCAAACGGTTTGGGGGCATTTCCCCGCCTACCGGCGTTTCGCGTATATTTAAAGCAAACGGCATTCCGAGGGCTACg                                               >  2:314302/1‑139 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAATCAAACTGCATTCCGAGGGCTACGGc                                             >  6:335280/1‑139 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACgg                                              >  3:411308/1‑138 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACgg                                              >  1:270812/1‑138 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACgg                                              >  7:219476/1‑138 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGc                                             >  7:413273/1‑139 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGc                                             <  8:65395/139‑1 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGATTATATATAAAGCAAACTGCATTCCGAGGGCTACgg                                              >  8:250270/1‑138 (MQ=255)                                                                                         ccGAAGTTTCCGTCTCTTTTCTGATTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTAACAGCCTACAGTCTTTTAGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACgg                                              >  4:390644/1‑138 (MQ=255)                                                                                            aaGTTTCCGTCTCGTTTCTGATTTGAATTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTATGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGAATTCCGAGGGCTACGGCAt                                           >  6:258970/1‑138 (MQ=255)                                                                                                           ttgtgaTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGAt                              <  1:273295/131‑1 (MQ=255)                                                                                                           ttctgaTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGAt                              <  7:357222/131‑1 (MQ=255)                                                                                                           ttctgaTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGAt                              <  6:346958/131‑1 (MQ=255)                                                                                                           ttctgaTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGAt                              <  7:429580/131‑1 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTTCGGCATCACCGTGAGTGATCTgcc                         <  8:145441/136‑3 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTgcc                         <  1:89711/136‑3 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTgcc                         <  4:247966/136‑3 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTgcc                         <  5:335280/136‑3 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTgcc                         <  7:368071/136‑3 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGccca                       <  3:2036/138‑2 (MQ=255)                                                                                                                aTTTGACTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGccca                       <  2:270812/138‑2 (MQ=255)                                                                                                                     aCTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc                                                         >  1:237715/1‑99 (MQ=255)                                                                                                                     aCTTGGATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATtcc                                                         <  2:237715/99‑1 (MQ=255)                                                                                                                          gATAACCCAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTc            >  3:228328/1‑139 (MQ=255)                                                                                                                               cccAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGCCTACGGCATCACCTTGAGTGATCTGCCCATATCCATATTCCGCCt       >  1:184168/1‑139 (MQ=255)                                                                                                                                ccAGGCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTc      <  3:93545/139‑1 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCg                                                        <  2:186640/85‑1 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCg                                                        >  1:186640/1‑85 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTgcc                         <  2:196273/116‑3 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATGTCCATATTCCGCCTCAACg  >  5:150431/1‑139 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCCCATATCCATATTCCGCCTCAACg  >  2:252878/1‑139 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATCACCGTGAGTGATCTGCACATATCCATATTCCGCCTCAACg  >  8:250403/1‑139 (MQ=255)                                                                                                                                    gCTTTTTAGCCAGCTGACCAAACGTTTTCTGTCCATTTCACAGCCTACAGTCTTTTCGCTTATATATAAAGCAAACTGCATTCCGAGGGCTACGGCATAACCGTGAGTGATCTgcc                         >  1:196273/1‑114 (MQ=255)                                                                                                                                        |                                                                                                                                       TCAGCAACTTTTCCTAATTCGTTGAGCACGATAAACTGAATCTTCCCGCCGCGGGTTTTCTTATCATTCATCATACGGTCCAATAGAACCGAAGTTTCCGTCTCATTTTTAATTTGACTTGGATAGCCCAGGCTTATTAGCCAACTGACCAAACGTTTCCTGTCCATTTCACAGCCCGCTGTCTTTTCGCTTATATATAAAGCAAACTGCATCCCGAGGGCTACGGCATCACCGTGAGTGATCTGTCCGTATCCATATTCAGCCTCAACG  >  CP006881/2195944‑2196213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑