Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,196,372	T→C	E198E (GAA→GAG)	U712_11005 ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,196,372	0	T	C	100.0%	286.9 / NA	88	E198E (GAA→GAG)	U712_11005	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/49);  total (39/49)

AGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGATATTCAGCAGCTCTTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGATCGAAGCTCTTTTTCAGGCAAAGAACGCAGGAAGTCTGTATCGTAGAGCACGGCCTTCGGCTGATAAAATGCACCGAT  >  CP006881/2196242‑2196506                                                                                                                                   |                                                                                                                                       agtaagCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGaaa                                                                                                                                <  6:364601/139‑1 (MQ=255) agtaagCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGaaa                                                                                                                                <  6:167524/139‑1 (MQ=255) agtaagCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGaa                                                                                                                                 <  1:63634/138‑1 (MQ=255)     atctctTATTCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  5:255989/137‑1 (MQ=255)     agatctTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGATTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  8:373918/136‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  8:376562/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  8:102934/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  2:282568/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  8:398478/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  1:418706/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  1:314302/139‑1 (MQ=255)     agCTCTTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGATCCTCCAGAAACGct                                                                                                                            <  8:7070/139‑1 (MQ=255)       ctctTATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATTTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGctct                                                                                                                          >  5:534/1‑139 (MQ=255)            aTCCCCTCTTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTc                                                                                                                       <  8:169803/137‑1 (MQ=255)                 ctctTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            >  2:252197/1‑127 (MQ=255)                 ctctTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  1:252197/127‑1 (MQ=255)                 ctctTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGAt                                                                                                                 >  3:393557/1‑138 (MQ=255)                 ctctTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGAt                                                                                                                 >  1:288696/1‑138 (MQ=255)                 ctctTCTTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGAt                                                                                                                 >  6:342059/1‑138 (MQ=255)                  tcttctTTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATa                                                                                                                >  2:336023/1‑138 (MQ=255)                    ttcttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCCGCTCCTCCAGAATCGCTCTGTCATatagaa                                                                                                               >  4:240224/1‑132 (MQ=255)                    ttcttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATaa                                                                                                               >  3:415324/1‑137 (MQ=255)                    tgcttTTTCATCCTGCTGAACGACAGCAGCATTAAAGGAAATGCCTTTAAAAATCATGTAATTCATATGATCATTAGTGATATCACGCAGTTAGTGGAGATTACGCAGCTCCTCCAGAAACACACTGTCATAGATaaat                                                                                                             >  6:341583/3‑138 (MQ=255)                      cttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAg                                                                                                            <  2:288696/138‑1 (MQ=255)                      cttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAg                                                                                                            <  8:280091/138‑1 (MQ=255)                      cttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAg                                                                                                            <  8:420745/138‑1 (MQ=255)                      cttTTTCATCCTGCTGAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGc                                                                                                           >  4:214255/1‑139 (MQ=255)                                ctgctgAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAAt                                                                                                  <  6:93915/138‑1 (MQ=255)                                ctgctgAACGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAAt                                                                                                  <  6:150431/138‑1 (MQ=255)                                      aaCGACAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCt                                                                                           >  6:224052/1‑139 (MQ=255)                                           cAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGccct                                                                                      >  7:113747/1‑137 (MQ=255)                                           cAGACGCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCAt                                                                                      >  4:19987/1‑139 (MQ=255)                                             gacgCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  3:214001/96‑1 (MQ=255)                                             gacgCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            >  4:214001/4‑99 (MQ=255)                                                gatttaATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCgg                                                                                 >  1:302177/3‑139 (MQ=255)                                                gCTTTAATTGAAATGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCg                                                                                  >  3:385308/1‑138 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTGGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCg                                                                                  <  2:323360/128‑1 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTGGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCg                                                                                  >  1:323360/1‑128 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            <  7:97583/86‑1 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGct                                                                                                                            >  8:97583/1‑86 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTACTCACTTCTGCCATTCCGGACCTAAGctc                                                                       >  1:235481/1‑139 (MQ=255)                                                          aaaTGCCTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGctc                                                                       >  7:54104/1‑139 (MQ=255)                                                            atgccTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGaa                                                                                                                                 <  6:310326/76‑1 (MQ=255)                                                            atgccTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGaa                                                                                                                                 >  5:310326/4‑79 (MQ=255)                                                            atgccTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCtt                                                                     >  3:359839/4‑139 (MQ=255)                                                            atgccTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCtt                                                                     >  6:306433/4‑139 (MQ=255)                                                            atgccTTTAAAAATCATGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTAtt                                                                     >  2:164147/4‑139 (MQ=255)                                                                           aTGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGaa                                                       <  7:159267/138‑1 (MQ=255)                                                                            tGTCATTCAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACg                                                     <  5:224052/139‑1 (MQ=255)                                                                                   cAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaa                                               <  7:405359/138‑1 (MQ=255)                                                                                   cAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaa                                               <  4:385308/138‑1 (MQ=255)                                                                                   cAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaa                                               <  4:45036/138‑1 (MQ=255)                                                                                   cAGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTACTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaa                                               <  5:30939/138‑1 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  3:19987/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  3:277398/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  6:534/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  2:302177/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  3:170242/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  5:381863/139‑3 (MQ=255)                                                                                    aGCTGATCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  5:342059/139‑3 (MQ=255)                                                                                         aTCATTAGTGATATCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTg                                          <  5:43134/137‑1 (MQ=255)                                                                                            attagTGATATCACGCAGGGAGCGGATATGCAGCAGCTCCTCCAGAAACGCTCTGTCATAGCTAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATca                                     <  2:184168/135‑2 (MQ=255)                                                                                                   atatGACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCa                              <  3:240224/139‑1 (MQ=255)                                                                                                   atatCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             >  8:255896/1‑122 (MQ=255)                                                                                                   atatCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGaaat                                             <  7:255896/124‑3 (MQ=255)                                                                                                   atatCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCa                              >  2:42930/1‑139 (MQ=255)                                                                                                   atatCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCa                              <  4:415324/139‑1 (MQ=255)                                                                                                    tatCACGCATTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCa                              <  1:136297/138‑1 (MQ=255)                                                                                                     atCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACg                            >  2:289843/1‑139 (MQ=255)                                                                                                     atCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACg                            >  8:443131/1‑139 (MQ=255)                                                                                                     atCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACg                            >  3:417972/1‑139 (MQ=255)                                                                                                      tCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTa                                        >  1:131177/1‑126 (MQ=255)                                                                                                      tCACGCAGTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTa                                        <  2:131177/126‑1 (MQ=255)                                                                                                             gTGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCgg                    <  7:443131/139‑1 (MQ=255)                                                                                                              tGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGc                   >  1:342685/1‑139 (MQ=255)                                                                                                              tGAGTGGATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGc                   <  4:417972/139‑1 (MQ=255)                                                                                                                agtggATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTg                 >  8:21880/4‑139 (MQ=255)                                                                                                                    gATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCttttt                                                                  <  1:101574/86‑1 (MQ=255)                                                                                                                    gATATTCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCttttt                                                                  >  2:101574/1‑86 (MQ=255)                                                                                                                        ttCAGCAGCTCCTCCAGAAACGCTCTGTCTTAGATAAAGGCGTGTTGAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATaaaacg         >  1:134123/1‑137 (MQ=255)                                                                                                                        ttCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATaaaacg         >  6:154597/1‑137 (MQ=255)                                                                                                                        ttCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATaaaacg         <  5:161377/139‑3 (MQ=255)                                                                                                                        ttCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATaaaacg         >  3:1627/1‑137 (MQ=255)                                                                                                                        ttCAGCAGCTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATaaaacg         >  2:424008/1‑137 (MQ=255)                                                                                                                           agcagcTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATAAAACGCa       <  3:214255/138‑1 (MQ=255)                                                                                                                           agcagcTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATAAAACGCa       <  1:403960/138‑1 (MQ=255)                                                                                                                            gcagcTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATAAAACGCAcc     >  7:408987/1‑139 (MQ=255)                                                                                                                            gcagcTCCTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGAACGGCTTTCGGCTGATAAAACGCAcc     >  8:408753/1‑139 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACg                                                     <  8:23304/87‑1 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCt                                           <  8:361580/97‑1 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCt                                           >  7:361580/1‑97 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATAAAACGCACCGAt  >  8:127623/1‑138 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTACGGACCTAAGCTCTTTTTCAGGCAGAGAACGCAGGAAATCTGTATCATAGAGCACGGCTTTCGGCTGATAAAACGCACCGAt  >  7:432426/1‑138 (MQ=255)                                                                                                                                ctcctcCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTCCCATTCCGGACCTAAGCTCTTTTTCAGGCAGAGAACg                                                     >  7:23304/1‑87 (MQ=255)                                                                                                                                   |                                                                                                                                       AGTAAGCTCTGATCCCCTCTTCTTTTTCATCCTGCTGAACGACAGATGCTTTTATTGAAATCCCTTTAAAAATCATGTCATTCAGCTGATCACTGGTGATATCACGCAGTGAGCGGATATTCAGCAGCTCTTCCAGAAACGCTCTGTCATAGATAAAAGCGTGTTTAATCACTTCTGCCATTCCGGATCGAAGCTCTTTTTCAGGCAAAGAACGCAGGAAGTCTGTATCGTAGAGCACGGCCTTCGGCTGATAAAATGCACCGAT  >  CP006881/2196242‑2196506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑