Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,196,713	G→A	L85L (CTG→TTG)	U712_11005 ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,196,713	0	G	A	100.0%	299.7 / NA	95	L85L (CTG→TTG)	U712_11005	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (48/47);  total (48/47)

GTAGGCATTTGGATAAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCTCCTACAACACCGCCACCGAACGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAGTTTTGTGTACATGTCCATGGATTTCGCCTGCTCTCCGCTCGGCACCGTCACTTTTTTCACGGGCCATTTTTCTTGCAGCAAATGAAGCATTTCATCACCATACAGGCGGTCAACCTCTTCATCCGTGACAAG  >  CP006881/2196577‑2196845                                                                                                                                         |                                                                                                                                     gtAGGCATTTGGATGAAATCAATGCCGCGCATAAAAGTTGCATCCACAAAACCGGCAAGATACCCGACCACACCGCCACCGAATGCGATGATTCATGAGGAGCGATCCATATTAAAACGAATCGCTTCACTCTTCAAtt                                                                                                                                    >  8:272765/1‑139 (MQ=255) gtAGGCATTTGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAAtt                                                                                                                                    <  4:141528/139‑1 (MQ=255) gtAGGCATTTGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAAtt                                                                                                                                    <  7:177498/139‑1 (MQ=255)    ggCATTTGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAAtttt                                                                                                                                  <  2:217526/138‑1 (MQ=255)       aTTTGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCAACAAATGCGATGATGCATCAGGAGCGATACATATAAAAACGAAACGCTTAACTCTGCAATTTtgtg                                                                                                                               >  3:76172/1‑138 (MQ=255)        tttGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTa                                                                                                                             >  7:128876/1‑139 (MQ=255)         ttGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGCGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTa                                                                                                                             >  1:166616/1‑138 (MQ=255)          tGGATGAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGAGGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACa                                                                                                                           >  3:440501/1‑139 (MQ=255)                aaaTCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCa                                                                                                                     <  6:126798/139‑1 (MQ=255)                 aaTCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCa                                                                                                                     <  8:21420/138‑1 (MQ=255)                  aTCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATg                                                                                                                   >  6:309372/1‑139 (MQ=255)                     aaTGCCGCGCATAAAAGTCGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGAt                                                                                                                <  7:440742/139‑1 (MQ=255)                           gcgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTAc                                                                                                                            >  1:114290/1‑121 (MQ=255)                           gcgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTAc                                                                                                                            <  2:114290/121‑1 (MQ=255)                           gcgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGcc                                                                                                          <  8:209444/139‑1 (MQ=255)                            cgcATAAAAGTGGCAGCCACAAACCCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCt                                                                                                         <  8:175879/139‑1 (MQ=255)                            cgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCt                                                                                                         >  7:222674/1‑139 (MQ=255)                            cgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCt                                                                                                         >  8:1719/1‑139 (MQ=255)                            cgcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCt                                                                                                         >  8:197165/1‑139 (MQ=255)                             gcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCa                                                                                                                     >  7:19544/1‑126 (MQ=255)                             gcATAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCa                                                                                                                     <  8:19544/126‑1 (MQ=255)                               aTAAAAGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGct                                                                                                      <  3:89521/139‑1 (MQ=255)                                  taaGTGGCAGCCACAAAACCGGCAAGATCCCCTACCACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCCTATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGctct                                                                                                    <  7:190281/137‑1 (MQ=255)                                  aaaGTGGCAGCCCCAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGctct                                                                                                    <  8:168896/138‑1 (MQ=255)                                  aaaGTGGCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGctct                                                                                                    <  7:171432/138‑1 (MQ=255)                                        gCAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCg                                                                                             >  5:388453/1‑139 (MQ=255)                                         cAGCCACAAAACCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCgg                                                                                            <  6:388453/139‑1 (MQ=255)                                                aaacCCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGAGGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTc                                                                                     <  8:273519/139‑1 (MQ=255)                                                aaaaCCGGCAAGATCCCCTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTc                                                                                     <  5:309372/139‑1 (MQ=255)                                                       gCAAGATCCCCTACAACCCCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCCCttttt                                                                              >  2:430434/1‑139 (MQ=255)                                                                ccTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGc                                                                      >  8:96948/1‑138 (MQ=255)                                                                ccTACAACACCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGc                                                                      >  3:425955/1‑138 (MQ=255)                                                                       cacCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGc                                                                                                           <  1:194490/94‑1 (MQ=255)                                                                       cacCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGc                                                                                                           >  2:194490/1‑94 (MQ=255)                                                                       cacCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTc                                                              >  4:330023/1‑139 (MQ=255)                                                                       cacCGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATATTGTGTACATGACCATGGATTTTGCCTGCTCTCCACTCGGTACCGGCCCTTTTTTCACAGGCCATTTTTc                                                              >  5:56828/1‑139 (MQ=255)                                                                          cGCCACCGCATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACCTGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  2:232100/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGct                                                                                                      <  1:394165/96‑1 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGct                                                                                                      >  2:394165/1‑96 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  5:370617/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  6:176561/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  4:337066/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  6:49484/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  8:365142/1‑139 (MQ=255)                                                                          cGCCACCGAATGCGATGATGAATGAGGAGCTCTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTACAGGTATTTTGCCTGCTCTCCAGACGGTACCGTCACGTTTTTGACAGTCCATTTTTCGTg                                                           >  4:210943/1‑139 (MQ=255)                                                                           gCCACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTgc                                                          <  5:77674/139‑1 (MQ=255)                                                                            ccACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTAc                                                                                                                            >  7:178493/1‑72 (MQ=255)                                                                            ccACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTAc                                                                                                                            <  8:178493/72‑1 (MQ=255)                                                                            ccACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTgca                                                         >  3:386991/1‑139 (MQ=255)                                                                            ccACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTgca                                                         >  4:2779/1‑139 (MQ=255)                                                                            ccACCGAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTg                                                           >  1:401595/1‑137 (MQ=255)                                                                                 gAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCaaa                                                    <  8:316763/139‑1 (MQ=255)                                                                                 gAATGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCaaa                                                    <  7:197165/139‑1 (MQ=255)                                                                                      cgATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAg                                               <  8:128876/139‑1 (MQ=255)                                                                                        atgatgCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCa                                             <  7:25835/139‑1 (MQ=255)                                                                                         tgatgCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCAt                                            <  5:375359/139‑1 (MQ=255)                                                                                           atgCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCAttt                                          <  3:330023/139‑1 (MQ=255)                                                                                           atgCAAGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCAttt                                          <  6:37708/139‑1 (MQ=255)                                                                                            tgCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTAACAGGCCATTTTTGTTTCAGGAAATGGAGCAttt                                          >  2:183336/1‑138 (MQ=255)                                                                                              cATGAGGAGCGATCCATCTGAAACCGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  2:134123/139‑1 (MQ=255)                                                                                              cATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  4:440501/139‑1 (MQ=255)                                                                                              cATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  4:386991/139‑1 (MQ=255)                                                                                                tGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAAtttt                                                                                                                                  <  8:84479/46‑1 (MQ=255)                                                                                                tGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAAtttt                                                                                                                                  >  7:84479/1‑46 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCtt                                                            >  1:245330/1‑109 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCtt                                                            <  2:245330/109‑1 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCAttt                                          >  7:418286/1‑127 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCAttt                                          <  8:418286/127‑1 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATa                                >  7:390072/1‑137 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATa                                >  2:384989/1‑137 (MQ=255)                                                                                                       cGATCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACa                              >  6:144746/1‑139 (MQ=255)                                                                                                         aTCCATATGAAAACGACTCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAgg                            <  2:166616/139‑1 (MQ=255)                                                                                                         aTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  3:150385/128‑1 (MQ=255)                                                                                                         aTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       >  4:150385/1‑128 (MQ=255)                                                                                                         aTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       >  4:184545/1‑128 (MQ=255)                                                                                                         aTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  3:184545/128‑1 (MQ=255)                                                                                                         aTCCATATGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACGTTTTTCACAGGCCATTTTTATTTCAGCAAATGGAGCATTTCATCACCAAACAgg                            >  4:309934/1‑139 (MQ=255)                                                                                                              tatGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       >  4:436675/1‑123 (MQ=255)                                                                                                              tatGAAAACGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  3:436675/123‑1 (MQ=255)                                                                                                                  aaaaCGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTATGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCAc                    <  6:107963/138‑1 (MQ=255)                                                                                                                    aaCGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGc                                                                                                           >  2:376915/1‑49 (MQ=255)                                                                                                                    aaCGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGc                                                                                                           <  1:376915/49‑1 (MQ=255)                                                                                                                    aaCGAATCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTACACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAATTGGAGCATTTCATCACCATACAGGCGGTCCActac                 >  2:105880/1‑136 (MQ=255)                                                                                                                    aaCGAATCGCTTCACTCTGCAATTTAGTGTACATGTCCATGGAATTTGAATCCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCAATTTTTCTTGCAGCAAATCGAGCATTTAATCACCATAAAGGAGGTccaatac                 >  8:141993/1‑133 (MQ=255)                                                                                                                        aaTCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGAt                                                                                                                >  8:283726/1‑40 (MQ=255)                                                                                                                        aaTCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGAt                                                                                                                <  7:283726/40‑1 (MQ=255)                                                                                                                        aaTCGCTTCACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATTGAGCATTTCATAACCATAAAGGCGGTCCActtctta              >  2:399354/1‑137 (MQ=255)                                                                                                                                cACTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGa      >  7:407860/1‑138 (MQ=255)                                                                                                                                 aCTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGAc     <  3:337066/138‑1 (MQ=255)                                                                                                                                 aCTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGAc     <  3:2779/138‑1 (MQ=255)                                                                                                                                 aCTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGAc     <  1:399354/138‑1 (MQ=255)                                                                                                                                 aCTCTGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGAc     <  7:365142/138‑1 (MQ=255)                                                                                                                                     tGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAATTGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACaaa  <  1:105880/137‑2 (MQ=255)                                                                                                                                     tGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       <  1:326695/100‑1 (MQ=255)                                                                                                                                     tGCAATTTTGTGTACATGTCCATGGATTTTGCCTGCTCTCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTtcat                                       >  2:326695/1‑100 (MQ=255)                                                                                                                                         |                                                                                                                                     GTAGGCATTTGGATAAAATCAATGCCGCGCATAAAAGTGGCAGCCACAAAACCGGCAAGATCTCCTACAACACCGCCACCGAACGCGATGATGCATGAGGAGCGATCCATATGAAAACGAATCGCTTCACTCTGCAGTTTTGTGTACATGTCCATGGATTTCGCCTGCTCTCCGCTCGGCACCGTCACTTTTTTCACGGGCCATTTTTCTTGCAGCAAATGAAGCATTTCATCACCATACAGGCGGTCAACCTCTTCATCCGTGACAAG  >  CP006881/2196577‑2196845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑