Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,196,872	G→A	L32L (CTA→TTA)	U712_11005 ←	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,196,872	0	G	A	100.0%	223.8 / NA	71	L32L (CTA→TTA)	U712_11005	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/27);  total (44/27)

TCGCCTGCTCTCCGCTCGGCACCGTCACTTTTTTCACGGGCCATTTTTCTTGCAGCAAATGAAGCATTTCATCACCATACAGGCGGTCAACCTCTTCATCCGTGACAAGCATAATCCGTGTTAAAGGCCTGTTTAGAGAGTTTAATAGTTCGCAAACCTCTTTTCTGATGCCTTGTCCGATAAAAACAGGATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTT  >  CP006881/2196737‑2197004                                                                                                                                        |                                                                                                                                     ttgCCTGCTCTCCACTCGGTACCGTCACTTTTTTAACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTaaaga                                                                                                                                   >  7:221057/3‑139 (MQ=255)        ctctCCACTCGGTACCGTCTCTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAAt                                                                                                                            <  5:96951/139‑1 (MQ=255)        ctctCCACTCGGTACCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTaa                                                                                                                             >  7:14756/1‑138 (MQ=255)                  ggtaCCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCa                                                                                                                    <  8:407860/134‑1 (MQ=255)                  ggtaCCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCAGACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGGTTAAAGAGGTTAATAGTTCGCaag                                                                                                                  >  3:421366/4‑138 (MQ=255)                   gtaCCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCAAACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCATTGTTAAAGGCCAGTTTAAAGAGGTTAATAGTTCGCaagg                                                                                                                 >  1:377173/3‑137 (MQ=255)                     aCCGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCGTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCct                                                                                                               >  2:259210/1‑139 (MQ=255)                      ccGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCctt                                                                                                              >  5:352448/1‑138 (MQ=255)                       cGTCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCcttt                                                                                                             >  5:184646/1‑137 (MQ=255)                        gTCACTTTTTTCACAGGACATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGGTAATAGTTCGCAAGCCtttt                                                                                                            <  3:210943/139‑1 (MQ=255)                         tCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTtttt                                                                                                           >  7:178163/1‑139 (MQ=255)                         tCACTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTtttt                                                                                                           >  3:394352/1‑139 (MQ=255)                          cccTTTTTTCACAGGCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCtttttt                                                                                                          <  4:78654/137‑1 (MQ=255)                                      aggCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGt                                                                                              <  6:352448/138‑1 (MQ=255)                                       ggCCATTTTTCTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTc                                                                                             >  5:132045/1‑139 (MQ=255)                                                 cTTGCAGCAAATGGAGCATTTCATCACCATACAGGCGGTCCAGTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTTATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    <  6:370617/138‑1 (MQ=255)                                                    gcagcaAATGGAGCATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAAc                                                                                   <  7:272765/136‑1 (MQ=255)                                                               aGCATTTCATCACCATACAGGCGGTCCCCTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAgg                                                                     >  5:437481/1‑139 (MQ=255)                                                                 cctttCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGaa                                                                   <  8:133392/137‑1 (MQ=255)                                                                 cATTTCATCACCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGaa                                                                   <  8:178163/139‑1 (MQ=255)                                                                      catcaCCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGt                                                              >  5:5800/1‑139 (MQ=255)                                                                          aCCATACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGa                                                          <  4:394352/139‑1 (MQ=255)                                                                              tACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCtttt                                                                                                            <  4:11015/85‑1 (MQ=255)                                                                              tACAGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACAt                                                      >  4:180980/1‑139 (MQ=255)                                                                                 aGGCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCa                                                   <  4:79396/139‑1 (MQ=255)                                                                                   gCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgt                                                 >  2:435076/1‑139 (MQ=255)                                                                                   gCGGTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCATTTTTTCTGATACCTTGt                                                                                              >  6:332648/1‑94 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGAt                                                                                         >  5:74050/1‑96 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    >  1:187419/1‑101 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    >  6:312817/1‑101 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    <  5:312817/101‑1 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    <  2:187419/101‑1 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              >  3:110896/1‑138 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              >  3:372414/1‑138 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              >  3:173230/1‑138 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAAAAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              >  7:337331/1‑138 (MQ=255)                                                                                      gTCCACTTCTTCATCCGTGACAAACATAATCATTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTAGCAAGCCTGATTTCTGATACCTTGTCCTATAAAAAAATGATATGACGAGGAAGCAGTTTGAACATGCAgtgct                                              >  7:334605/1‑137 (MQ=255)                                                                                        ccacatctTCATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATCCAGTGtctt                                            >  4:16210/6‑139 (MQ=255)                                                                                                 cATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAAt                                   >  7:424264/1‑139 (MQ=255)                                                                                                 cATCCGTGACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGCATATTACGAGGAAGCAGTTTGCACATGCAGTGTCTTCATTAAAAt                                   >  1:1517/1‑139 (MQ=255)                                                                                                        gACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    <  5:200467/83‑1 (MQ=255)                                                                                                        gACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATaaaaa                                                                                    >  6:200467/1‑83 (MQ=255)                                                                                                        gACAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTc                             >  7:18319/1‑138 (MQ=255)                                                                                                         aCAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAg                                                                  <  8:62946/100‑1 (MQ=255)                                                                                                         aCAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAg                                                                  >  7:62946/1‑100 (MQ=255)                                                                                                         aCAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAAc                                                        >  7:370406/1‑110 (MQ=255)                                                                                                         aCAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAAc                                                        <  8:370406/110‑1 (MQ=255)                                                                                                         aCAAACATAATCCTTGTTAAAGGCCTCTTTAAAGAGGTTAATAGTTCGAAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGAAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGa                           >  7:119463/1‑139 (MQ=255)                                                                                                          cAAACATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGGCTTCATTAAAATTCCCTCGa                           <  6:56828/138‑1 (MQ=255)                                                                                                            aacATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAg                        <  2:401595/137‑1 (MQ=255)                                                                                                            aacATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAg                        >  8:32791/3‑139 (MQ=255)                                                                                                              cATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGtt                      <  2:377173/139‑1 (MQ=255)                                                                                                              cATAATCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGtt                      <  1:435076/139‑1 (MQ=255)                                                                                                                   tCCTTGTTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTtctc                 <  3:176787/139‑1 (MQ=255)                                                                                                                       tgtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGa                           <  7:66748/125‑1 (MQ=255)                                                                                                                       tgtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGa                           >  8:66748/1‑125 (MQ=255)                                                                                                                       tgtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCatat             >  4:375975/1‑139 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCatat             >  3:256387/1‑138 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCatat             >  3:85840/1‑138 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCatat             >  4:118271/1‑138 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATAtt            >  1:386925/1‑139 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATAtt            >  4:130671/1‑139 (MQ=255)                                                                                                                        gtTAAAGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGAAACCTTGTCCGAAAACAACAGGATATGACGAGGAAGCAGATTGAACATGCAGTGTCTTCATAAAAATTCCCTCGACAGTTTTCTCATAtt            >  2:340057/1‑139 (MQ=255)                                                                                                                            aacgCCTGTTTAAAGAGGATAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAACACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATtctc         <  5:97688/135‑1 (MQ=255)                                                                                                                            aaGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATtctc         <  8:18319/138‑1 (MQ=255)                                                                                                                             aGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCca       >  2:373438/1‑139 (MQ=255)                                                                                                                             aGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCca       >  1:39212/1‑139 (MQ=255)                                                                                                                             aGGCCTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCca       >  4:224727/1‑139 (MQ=255)                                                                                                                                ccTGTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACAt    <  4:85840/139‑1 (MQ=255)                                                                                                                                   gTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              <  6:437461/94‑2 (MQ=255)                                                                                                                                   gTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAgtgtc                                              >  5:437461/1‑93 (MQ=255)                                                                                                                                   gTTTAAAGAGGTTAATAGTTCGCAAGCCTTTTTTCTGATACCTTGTCCGATAAAAACAGGATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACAttt  >  7:395324/1‑138 (MQ=255)                                                                                                                                        |                                                                                                                                     TCGCCTGCTCTCCGCTCGGCACCGTCACTTTTTTCACGGGCCATTTTTCTTGCAGCAAATGAAGCATTTCATCACCATACAGGCGGTCAACCTCTTCATCCGTGACAAGCATAATCCGTGTTAAAGGCCTGTTTAGAGAGTTTAATAGTTCGCAAACCTCTTTTCTGATGCCTTGTCCGATAAAAACAGGATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTT  >  CP006881/2196737‑2197004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑