Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,061	C→T	E359E (GAG→GAA)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,061	0	C	T	98.6%	222.0 / ‑5.7	73	E359E (GAG→GAA)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (31/41);  total (31/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGT  >  CP006881/2196926‑2197189                                                                                                                                        |                                                                                                                                 gATATGACGAGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTcc                                                                                                                               >  2:35253/1‑139 (MQ=255)         gaGGAAGCAGTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGa                                                                                                                        >  5:224281/1‑138 (MQ=255)                  gTTTGAACATGCAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTc                                                                                                              <  8:14756/139‑1 (MQ=255)                   tttGATCATGCTGTGTCTTAATTAAAATTCCCTGGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGGCAATTTGATATAATGCTATTTTTTCACCGACTGCGTTCGCAATTTCCCATGCGACAGCAGCTTc                                                                                                              <  8:119463/138‑1 (MQ=255)                             cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTccac                                                                                                   >  2:126382/1‑139 (MQ=255)                             cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                                                   >  6:169225/1‑139 (MQ=255)                             cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                                                   >  7:403107/1‑139 (MQ=255)                               gtgtCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTATCCACATTTTCTCTAATGCGGTCAATTTGATCTAAACCTAATGTTCCAACGACTGCGTTCGCAATTTCCCACGCGACAGCCGCTTCAGCGACTACACtt                                                                                                 <  6:33885/139‑1 (MQ=255)                                 gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAAGTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                                               >  7:151115/4‑139 (MQ=255)                                 gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGACAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                                               >  7:348650/4‑139 (MQ=255)                                  tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGcga                                                                                                         >  8:386365/3‑126 (MQ=255)                                  tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGcga                                                                                                         <  7:386365/126‑3 (MQ=255)                                  tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGcc                                                                                              >  2:59412/3‑139 (MQ=255)                                      aataaaaaTGCCCGCCCCAGTTTACTCATATTCTCCCCATTTTCTCAAATGCGGCAAATTTCATAAAATCCGAATTGTCCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                            <  6:5800/133‑1 (MQ=255)                                       aTTAACATGCCCTCGACAGTTTTCTCAAATTCTCCACATTTGCTCAAATGCGGGCAATTTGATCAAATCCGAATTGTTCAACGACTGCGTGCGCAAATTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                         <  6:437481/139‑1 (MQ=255)                                       aTTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAg                                                                                          >  4:258062/1‑138 (MQ=255)                                        ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACCCTTGCCGCAgg                                                                                         >  3:108441/1‑138 (MQ=255)                                        ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGAATGCGTTAGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                         >  8:146871/1‑138 (MQ=255)                                          aaatTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  2:39212/138‑3 (MQ=255)                                          aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGGGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  8:151115/138‑3 (MQ=255)                                          aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  8:403107/138‑3 (MQ=255)                                          aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  3:224727/138‑3 (MQ=255)                                          aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  1:373438/138‑3 (MQ=255)                                          aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTGCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                       <  7:273677/138‑3 (MQ=255)                                                 ccTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                               <  2:247110/139‑1 (MQ=255)                                                 ccTCGACAGTTTTCTCATATTCTCAACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                               <  8:337331/139‑1 (MQ=255)                                                 ccTCGACAGCTTTCTCATAATCGCCACATATACGCTAATGCCGTCAATATGACCTAAGCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                               <  3:164238/139‑1 (MQ=255)                                                   tCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCt                                                                             >  5:261707/1‑139 (MQ=255)                                                         gTTTTCTCATATTCTCCATTTTTTCTCTTATGCGGTCAACTTCAACTAATCCCAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  1:340057/139‑1 (MQ=255)                                                         gTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  5:169225/139‑1 (MQ=255)                                                         gTTTCCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCAAATCCGACTTGTTCAACGACTGCGGTCTCAATCTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  7:58997/139‑1 (MQ=255)                                                                    ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGc                                                            >  6:405785/1‑139 (MQ=255)                                                                    ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                            >  6:358060/1‑107 (MQ=255)                                                                    ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                            <  5:358060/107‑1 (MQ=255)                                                                    ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                         >  7:214238/1‑110 (MQ=255)                                                                    ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                         <  8:214238/110‑1 (MQ=255)                                                                         cacaTTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTgg                                                         <  8:424264/137‑1 (MQ=255)                                                                                      aTGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCt                                           <  1:35253/138‑1 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       >  2:359962/1‑105 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       >  2:365963/1‑105 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  1:415186/105‑1 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       >  2:415186/1‑105 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  1:365963/105‑1 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  1:359962/105‑1 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGtt                                     >  7:301636/1‑139 (MQ=255)                                                                                           gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGATCAATGCTGGCAGAAAACGGTTCTTTCGtt                                     >  6:448148/1‑139 (MQ=255)                                                                                              aaTTTGATCTAATCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTc                                   <  8:404363/137‑1 (MQ=255)                                                                                                 tttaTCTATTCCCAATTGTTCAACCCCTGGGTGCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAat                                <  8:395324/135‑1 (MQ=255)                                                                                                         atccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAACCGGTTCTTTCGTTTCAATATCGAca                         <  3:375975/135‑2 (MQ=255)                                                                                                          tccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGTACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACtt                      >  1:49339/2‑139 (MQ=255)                                                                                                          tccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACtt                      >  3:163237/2‑139 (MQ=255)                                                                                                              aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  3:150614/139‑1 (MQ=255)                                                                                                              aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  3:155135/139‑1 (MQ=255)                                                                                                              aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  4:315015/139‑1 (MQ=255)                                                                                                               aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  2:88981/138‑1 (MQ=255)                                                                                                               aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAg                 <  6:150290/139‑1 (MQ=255)                                                                                                               aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAg                 <  6:132045/139‑1 (MQ=255)                                                                                                                ttGTTCAACGACTGCGTTCGCAAATTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGc                <  1:233827/139‑1 (MQ=255)                                                                                                                   ttCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGaa                                                    >  1:383562/1‑100 (MQ=255)                                                                                                                   ttCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGaa                                                    <  2:383562/100‑1 (MQ=255)                                                                                                                        cGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTtata        >  5:214659/1‑139 (MQ=255)                                                                                                                          aCTGCGTTCGCAATTTCCCACGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACa      <  1:160536/139‑1 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       >  5:13872/1‑71 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                       <  6:13872/71‑1 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCtt                                          >  6:78920/1‑100 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCtt                                          <  5:78920/100‑1 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  >  2:237043/1‑124 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  7:177891/124‑1 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  >  8:177891/1‑124 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                  <  1:237043/124‑1 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAg   >  7:321943/1‑139 (MQ=255)                                                                                                                             gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGGTTATACAAAg   >  5:288502/1‑139 (MQ=255)                                                                                                                              cGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGt  <  3:130671/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GATACGACGAGGAAGCGGTTTGAACATGCAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGT  >  CP006881/2196926‑2197189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑