Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,085	T→G	V351V (GTA→GTC)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,085	0	T	G	100.0%	248.9 / NA	76	V351V (GTA→GTC)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (31/45);  total (31/45)

CAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCG  >  CP006881/2196954‑2197213                                                                                                                                    |                                                                                                                                 cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTccac                                                                                                                           >  2:126382/1‑139 (MQ=255) cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                                                                           >  6:169225/1‑139 (MQ=255) cAGTGTCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTacac                                                                                                                           >  7:403107/1‑139 (MQ=255)   gtgtCTTCATTAAAATTCCCTCGACAGTTTTCTCATATTATCCACATTTTCTCTAATGCGGTCAATTTGATCTAAACCTAATGTTCCAACGACTGCGTTCGCAATTTCCCACGCGACAGCCGCTTCAGCGACTACACtt                                                                                                                         <  6:33885/139‑1 (MQ=255)     gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAAGTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                                                                       >  7:151115/4‑139 (MQ=255)     gtcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGACAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGc                                                                                                                       >  7:348650/4‑139 (MQ=255)      tcttCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGcc                                                                                                                      >  2:59412/3‑139 (MQ=255)          aataaaaaTGCCCGCCCCAGTTTACTCATATTCTCCCCATTTTCTCAAATGCGGCAAATTTCATAAAATCCGAATTGTCCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                                                    <  6:5800/133‑1 (MQ=255)           aTTAACATGCCCTCGACAGTTTTCTCAAATTCTCCACATTTGCTCAAATGCGGGCAATTTGATCAAATCCGAATTGTTCAACGACTGCGTGCGCAAATTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                                                 <  6:437481/139‑1 (MQ=255)           aTTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAg                                                                                                                  >  4:258062/1‑138 (MQ=255)            ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACCCTTGCCGCAgg                                                                                                                 >  3:108441/1‑138 (MQ=255)            ttAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGAATGCGTTAGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                                                 >  8:146871/1‑138 (MQ=255)              aaatTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  2:39212/138‑3 (MQ=255)              aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGGGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  8:151115/138‑3 (MQ=255)              aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  3:224727/138‑3 (MQ=255)              aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  8:403107/138‑3 (MQ=255)              aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  1:373438/138‑3 (MQ=255)              aaaaTTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTGCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAggaa                                                                                                               <  7:273677/138‑3 (MQ=255)                     ccTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                                                       <  2:247110/139‑1 (MQ=255)                     ccTCGACAGTTTTCTCATATTCTCAACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                                                       <  8:337331/139‑1 (MQ=255)                     ccTCGACAGCTTTCTCATAATCGCCACATATACGCTAATGCCGTCAATATGACCTAAGCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAg                                                                                                       <  3:164238/139‑1 (MQ=255)                       tCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCt                                                                                                     >  5:261707/1‑139 (MQ=255)                             gTTTTCTCATATTCTCCATTTTTTCTCTTATGCGGTCAACTTCAACTAATCCCAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  1:340057/139‑1 (MQ=255)                             gTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  5:169225/139‑1 (MQ=255)                             gTTTCCTCATATTCTCCACATTTTCTCTAATGCGGTCAATTTGATCAAATCCGACTTGTTCAACGACTGCGGTCTCAATCTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  7:58997/139‑1 (MQ=255)                                        ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGCCTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGc                                                                                    >  6:405785/1‑139 (MQ=255)                                        ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                                                    <  5:358060/107‑1 (MQ=255)                                        ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGc                                                                                                                    >  6:358060/1‑107 (MQ=255)                                        ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                                                 >  7:214238/1‑110 (MQ=255)                                        ttCTCCACATTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAgg                                                                                                                 <  8:214238/110‑1 (MQ=255)                                             cacaTTTTCTCTAATGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTgg                                                                                 <  8:424264/137‑1 (MQ=255)                                                          aTGCGGTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCt                                                                   <  1:35253/138‑1 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  1:359962/105‑1 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  1:365963/105‑1 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               >  2:415186/1‑105 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               >  2:365963/1‑105 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               >  2:359962/1‑105 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  1:415186/105‑1 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGtt                                                             >  7:301636/1‑139 (MQ=255)                                                               gTCAATTTGATCTAATCCGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGATCAATGCTGGCAGAAAACGGTTCTTTCGtt                                                             >  6:448148/1‑139 (MQ=255)                                                                  aaTTTGATCTAATCCGACTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTc                                                           <  8:404363/137‑1 (MQ=255)                                                                     tttaTCTATTCCCAATTGTTCAACCCCTGGGTGCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAat                                                        <  8:395324/135‑1 (MQ=255)                                                                             atccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAACCGGTTCTTTCGTTTCAATATCGAca                                                 <  3:375975/135‑2 (MQ=255)                                                                              tccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGTACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACtt                                              >  1:49339/2‑139 (MQ=255)                                                                              tccGAATTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACtt                                              >  3:163237/2‑139 (MQ=255)                                                                                  aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  4:315015/139‑1 (MQ=255)                                                                                  aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  3:150614/139‑1 (MQ=255)                                                                                  aaTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  3:155135/139‑1 (MQ=255)                                                                                   aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  2:88981/138‑1 (MQ=255)                                                                                   aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAg                                         <  6:132045/139‑1 (MQ=255)                                                                                   aTTGTTCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAg                                         <  6:150290/139‑1 (MQ=255)                                                                                    ttGTTCAACGACTGCGTTCGCAAATTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGc                                        <  1:233827/139‑1 (MQ=255)                                                                                       ttCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGaa                                                                            >  1:383562/1‑100 (MQ=255)                                                                                       ttCAACGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGaa                                                                            <  2:383562/100‑1 (MQ=255)                                                                                            cGACTGCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTtata                                >  5:214659/1‑139 (MQ=255)                                                                                              aCTGCGTTCGCAATTTCCCACGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACa                              <  1:160536/139‑1 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               <  6:13872/71‑1 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGa                                                                                               >  5:13872/1‑71 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCtt                                                                  >  6:78920/1‑100 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCtt                                                                  <  5:78920/100‑1 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  1:237043/124‑1 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          >  2:237043/1‑124 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          >  8:177891/1‑124 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCa                                          <  7:177891/124‑1 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAg                           >  7:321943/1‑139 (MQ=255)                                                                                                 gCGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGGTTATACAAAg                           >  5:288502/1‑139 (MQ=255)                                                                                                  cGTTCGCAATTTCCCAAGCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGt                          <  3:130671/139‑1 (MQ=255)                                                                                                                caagCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGATCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCa            <  6:184646/136‑1 (MQ=255)                                                                                                                   gCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATcga                                                   >  3:408099/1‑95 (MQ=255)                                                                                                                   gCGACAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATcga                                                   <  4:408099/97‑3 (MQ=255)                                                                                                                       cAGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTcc     >  4:320722/1‑139 (MQ=255)                                                                                                                        aGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCcg    <  3:258062/139‑1 (MQ=255)                                                                                                                        aGCCGCTTCAGCGACTACACTTGCCGCAGGAACAGAACAGCTGTCTGAACGTTCAATGCTGGCACAAAACGGTTCTTTCGTTTCAATATCGACACTATTCAGCGGATTATACAAAGTCGTAATCGGCTTCATGACACcg    >  2:229060/1‑139 (MQ=255)                                                                                                                          ccGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCcgcg  <  3:180980/139‑1 (MQ=255)                                                                                                                          ccGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCcgcg  <  8:301636/139‑1 (MQ=255)                                                                                                                          ccGCTTCAGCGACTACACTTGCCGCAGGAACAGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCcgcg  <  4:110896/139‑1 (MQ=255)                                                                                                                                    |                                                                                                                                 CAGTGTTTTCATTAAAATTCCCTCGACAGTTTTCTCATATTCTCCACATTTTCTCTAATGCGGTCGATTTGATCTAAGCCGAATTGTTCAACGACTGCGTTCGCAATCTCCCATGCGACAGCCGCTTCAGCTACTACACTTGCCGCAGGCACTGCACAGCTGTCTGAACGTTCAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCG  >  CP006881/2196954‑2197213

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑