Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,259	G→A	L293L (CTC→CTT)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,259	0	G	A	100.0%	173.1 / NA	59	L293L (CTC→CTT)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/24);  total (35/24)

CAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGA  >  CP006881/2197126‑2197387                                                                                                                                      |                                                                                                                                 cAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCt                                                                                                                             >  7:445879/1‑139 (MQ=255)    tGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGtt                                                                                                                          <  4:213793/139‑1 (MQ=255)      cTGGCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGttag                                                                                                                        <  8:321943/139‑3 (MQ=255)       ttgCAGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTAGGAATCGTCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGt                                                                                                                       <  5:448148/137‑1 (MQ=255)          cAGAAAACGGTTCTTTCGTTTCAATATCCACACTTTTCAGCGGTTTATACAAAGTCGTAAGCGGCTTCATGCCTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCATACCAGTCCGACAATTCTGttattag                                                                                                                     >  6:99702/1‑133 (MQ=255)           aGAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgca                                                                                                                   <  3:256493/139‑2 (MQ=255)           aGAAAACGGTTCTTTAGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgca                                                                                                                   <  8:445879/139‑2 (MQ=255)            gAAAACGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAgcac                                                                                                                  <  2:49339/139‑3 (MQ=255)             aaaaCGGTTCTTTCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGATTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGACTTCCAGTCCGCCAAGTCTGTTAGTAgcaag                                                                                                                 >  8:233716/1‑136 (MQ=255)                 cGGTTCTTTCGTTTCAATCTCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGtg                                                                                                             <  6:261707/139‑2 (MQ=255)                       tttCGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGGCTGTTAGTAGCACGGGTGTATcct                                                                                                       >  2:375946/1‑138 (MQ=255)                          cGTTTCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCAGCGTACAACAATCGGCATCCCTGTCGTAATTCCGCCTTCCAGTCAGCCAAGTCTGTTATTAGCACGGGTGTTTCCtttt                                                                                                    >  6:315636/1‑139 (MQ=255)                            tttCAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTc                                                                                                  >  7:337300/1‑139 (MQ=255)                               cAATATCGACACTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCt                                                                                                <  7:146871/138‑1 (MQ=255)                                      gacacTTTTAAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATAGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCaa                                                                                        <  6:288502/135‑1 (MQ=255)                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                                                 >  3:14687/1‑139 (MQ=255)                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtct                                                                                 >  6:91346/1‑139 (MQ=255)                                             ttCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                  <  1:126382/138‑1 (MQ=255)                                                  ctgTTTATAGAAAGTCGGAATCGTCTTCATGACTCCGCGGACAACAATCGTCATCCCTGTCGTCATGCCGCCTTCGAGTCCGACAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                            <  6:134870/137‑1 (MQ=255)                                                     tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTATTAGCACGGGTGTATCATTTTTCCTCTTCCCAAATGATATCGTCATg                                                                          >  5:134870/1‑138 (MQ=255)                                                     tttATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATgg                                                                         >  6:412915/1‑139 (MQ=255)                                                       tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTTGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGAc                                                                       >  4:423553/1‑139 (MQ=255)                                                       tataCAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGa                                                                        <  8:348650/138‑1 (MQ=255)                                                             aaGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                                                 <  5:91346/139‑1 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                  <  8:104759/121‑1 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                  >  7:104759/1‑121 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                  >  5:72761/1‑121 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                >  1:17903/1‑139 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                >  2:7603/1‑139 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCtt                                                                >  1:401808/1‑139 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCt                                                                 >  4:126194/1‑138 (MQ=255)                                                              aGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGGGTATCCTTTTTCCTCGTCCCAAATGAtc                                                                                  <  6:72761/121‑1 (MQ=255)                                                                        ggCTTCATGACTCCGCGGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATttcgcc                                                      >  8:238416/1‑136 (MQ=255)                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                         >  5:186762/1‑139 (MQ=255)                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                         >  1:216402/1‑139 (MQ=255)                                                                                     cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaa                                          >  7:351700/1‑138 (MQ=255)                                                                                       cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaatc                                       >  7:302402/1‑137 (MQ=255)                                                                                       cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCACTCCGCCAACTCTGTTAGTATCACCGGTGTATACTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTACATTTCGCCCTGCTGCCTCaaatc                                       >  4:151282/1‑137 (MQ=255)                                                                                                 tCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGACCTGCTGCCTCAAATCCGATGcgcaa                             >  5:385499/1‑135 (MQ=255)                                                                                                     cATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTcc                         <  5:405785/139‑1 (MQ=255)                                                                                                      atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                        >  8:287665/4‑139 (MQ=255)                                                                                                      atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                        <  7:238416/136‑1 (MQ=255)                                                                                                      atcccTGTCGTCATGACGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                        <  1:375946/136‑1 (MQ=255)                                                                                                       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                       <  5:412915/137‑1 (MQ=255)                                                                                                       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                       <  5:59107/137‑1 (MQ=255)                                                                                                       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTATCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                       <  5:99702/137‑1 (MQ=255)                                                                                                        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                            >  7:165719/2‑85 (MQ=255)                                                                                                        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCa                                                                            <  8:165719/84‑1 (MQ=255)                                                                                                        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                      >  5:16561/2‑139 (MQ=255)                                                                                                        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                      >  4:399272/2‑139 (MQ=255)                                                                                                         ccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCGCAAATCCGATGCCGAATTCCacac                     >  2:59021/1‑139 (MQ=255)                                                                                                                  aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg            >  8:21503/1‑139 (MQ=255)                                                                                                                  aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg            >  1:364205/1‑139 (MQ=255)                                                                                                                   tGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGc           >  8:103443/1‑139 (MQ=255)                                                                                                                    gccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCg          >  7:276400/1‑139 (MQ=255)                                                                                                                     ccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGt         >  8:72275/1‑139 (MQ=255)                                                                                                                       gccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa       <  2:397004/139‑1 (MQ=255)                                                                                                                          ttCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAAtt    >  4:343039/1‑139 (MQ=255)                                                                                                                             cAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGa  <  6:16561/138‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 CAATGCTGGCAGAAAACGGTTCTTTCGTTTCAATATCTACGCTTTTCAGCGGTTTATACAAAGTCGGAATCGGCTTCATGACTCCGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGA  >  CP006881/2197126‑2197387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑