Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,343	T→C	E265E (GAA→GAG)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,343	0	T	C	100.0%	283.1 / NA	87	E265E (GAA→GAG)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/43);  total (44/43)

CGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGAC  >  CP006881/2197210‑2197474                                                                                                                                      |                                                                                                                                    cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                                                                                                                >  1:216402/1‑139 (MQ=255) cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaa                                                                                                                                >  5:186762/1‑139 (MQ=255) cgcgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaa                                                                                                                                 >  7:351700/1‑138 (MQ=255)   cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCaaatc                                                                                                                              >  7:302402/1‑137 (MQ=255)   cgGACAACAATCGGCATCCCTGTCGTCATGCCGCCTTCCACTCCGCCAACTCTGTTAGTATCACCGGTGTATACTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTACATTTCGCCCTGCTGCCTCaaatc                                                                                                                              >  4:151282/1‑137 (MQ=255)             tCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGACCTGCTGCCTCAAATCCGATGcgcaa                                                                                                                    >  5:385499/1‑135 (MQ=255)                 cATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTcc                                                                                                                <  5:405785/139‑1 (MQ=255)                  atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               >  8:287665/4‑139 (MQ=255)                  atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               <  7:238416/136‑1 (MQ=255)                  atcccTGTCGTCATGACGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               <  1:375946/136‑1 (MQ=255)                   tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              <  5:59107/137‑1 (MQ=255)                   tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              <  5:412915/137‑1 (MQ=255)                   tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTATCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              <  5:99702/137‑1 (MQ=255)                    cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                                                                                             >  4:399272/2‑139 (MQ=255)                    cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                                                                                             >  5:16561/2‑139 (MQ=255)                     ccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCGCAAATCCGATGCCGAATTCCacac                                                                                                            >  2:59021/1‑139 (MQ=255)                              aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                                                                                   >  8:21503/1‑139 (MQ=255)                              aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                                                                                   >  1:364205/1‑139 (MQ=255)                               tGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGc                                                                                                  >  8:103443/1‑139 (MQ=255)                                gccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCg                                                                                                 >  7:276400/1‑139 (MQ=255)                                 ccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGt                                                                                                >  8:72275/1‑139 (MQ=255)                                   gccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                              <  2:397004/139‑1 (MQ=255)                                      ttCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAAtt                                                                                           >  4:343039/1‑139 (MQ=255)                                         cAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGa                                                                                         <  6:16561/138‑1 (MQ=255)                                              cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               >  3:236886/6‑111 (MQ=255)                                              cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               <  4:236886/106‑1 (MQ=255)                                                 caaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAAcagc                                                                                >  2:301238/3‑139 (MQ=255)                                                         ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc                                                                          >  6:445186/4‑137 (MQ=255)                                                         ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTACCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc                                                                          >  3:183935/4‑137 (MQ=255)                                                            ggaGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt                                                                     <  7:306941/137‑1 (MQ=255)                                                            gTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt                                                                     <  2:401808/139‑1 (MQ=255)                                                              aGCACGGGTGTATCCTTTTTCATCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCAGATTCCTAAATCCACACCTTTAAATGCGTTAATTTACAGAACAGCGCCAGCAAGCttc                                                                   >  8:267165/1‑138 (MQ=255)                                                               gcacgGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGc                                                                  >  5:117504/4‑139 (MQ=255)                                                               gcacgGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTAAATTGACAGAACAGCGGCAGCAAGCTTg                                                                   <  3:126194/135‑1 (MQ=255)                                                               gcacgGGTGTATCCTTTTTCCTCGCCCCAAATGAGCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTg                                                                   <  5:315636/135‑1 (MQ=255)                                                                  cgGGTGTATCCTTTTTCCTCGGCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCtat                                                               <  6:224281/139‑3 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               >  7:153183/1‑83 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                               <  8:153183/83‑1 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       <  6:186762/139‑1 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       <  7:103443/139‑1 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       <  4:163237/139‑1 (MQ=255)                                                                          tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       <  8:302402/139‑1 (MQ=255)                                                                              ttttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                   >  7:188210/1‑139 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       <  7:360060/134‑1 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                       >  8:360060/1‑134 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCgg                                                    <  6:214659/137‑1 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                   >  3:202173/1‑138 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                   >  1:256022/1‑138 (MQ=255)                                                                               tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                   >  6:395109/1‑138 (MQ=255)                                                                               tttCCTCGTCCCAAAAGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCgg                                                    <  1:89131/137‑1 (MQ=255)                                                                               attCCTCGTCCCAAATGATCTCGTCATGTACTTCCCTTCCATTTAGCCCTGCTGCCTCAAATCCGATGCCGAATTACACAACTATAAAAGCGTTAATTGACAGAACAGAGCCAGAAAGCTTGCTATCCAGCTTGCGGt                                                   >  4:378828/2‑138 (MQ=255)                                                                                   cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               <  8:188210/138‑1 (MQ=255)                                                                                   cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               <  7:72275/138‑1 (MQ=255)                                                                                   cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               <  7:21503/138‑1 (MQ=255)                                                                                   cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               <  3:343039/138‑1 (MQ=255)                                                                                   cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACAACTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCCGCTTGCGGTCATa                                               <  5:402008/138‑1 (MQ=255)                                                                                     cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                            >  7:147048/1‑139 (MQ=255)                                                                                     cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                            >  1:200164/1‑139 (MQ=255)                                                                                     cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                            >  3:234252/1‑139 (MQ=255)                                                                                     cGTCCCAAATGATCTCGTCATGGACTTAGCTTCCATTTCGCCCTGCTGCCTCAAAAACGATGCCGAATTCCACACCTTTAAATGAGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCAAGCTTGCGGTCATAATg                                            >  4:41984/1‑139 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              <  7:431307/72‑1 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              >  8:431307/1‑72 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                              >  3:314223/1‑88 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                              <  4:314223/88‑1 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                            >  3:73724/1‑138 (MQ=255)                                                                                      gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATACCACACCTATAAATGCGTTAAATGACAGAACAGCCCCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                            >  6:402008/1‑138 (MQ=255)                                                                                       tCCCTAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAACTCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGa                                          <  5:445186/139‑1 (MQ=255)                                                                                       tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               <  6:1501/134‑1 (MQ=255)                                                                                       tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                               >  5:1501/1‑134 (MQ=255)                                                                                       tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGa                                          <  1:301238/139‑1 (MQ=255)                                                                                            aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                     <  2:216402/139‑1 (MQ=255)                                                                                            aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                     <  8:351700/139‑1 (MQ=255)                                                                                            aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                     <  3:399272/139‑1 (MQ=255)                                                                                                  ctcGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGccc                               >  3:131313/1‑138 (MQ=255)                                                                                                     gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                     <  4:24079/130‑1 (MQ=255)                                                                                                     gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                     >  3:24079/1‑130 (MQ=255)                                                                                                     gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                <  3:429308/135‑1 (MQ=255)                                                                                                               tCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              >  8:87853/1‑47 (MQ=255)                                                                                                               tCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                              <  7:87853/47‑1 (MQ=255)                                                                                                                cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                <  8:92444/124‑1 (MQ=255)                                                                                                                cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                >  7:92444/1‑124 (MQ=255)                                                                                                                cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGc                   <  4:73724/137‑1 (MQ=255)                                                                                                                cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                 >  4:438631/1‑139 (MQ=255)                                                                                                                cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                 >  1:106197/1‑139 (MQ=255)                                                                                                                     ccATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTccc             <  2:256022/138‑1 (MQ=255)                                                                                                                      cATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt            <  4:32975/138‑1 (MQ=255)                                                                                                                      cATTTCGCCCTGCTGACTCAAATCCGCTGCAGAATTCCACTCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCATCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt            <  7:267165/138‑1 (MQ=255)                                                                                                                           cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa       <  4:183935/132‑1 (MQ=255)                                                                                                                           cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa       <  6:117504/132‑1 (MQ=255)                                                                                                                            gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt     <  2:364205/134‑1 (MQ=255)                                                                                                                            gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt     <  8:276400/134‑1 (MQ=255)                                                                                                                            gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt     <  8:337300/134‑1 (MQ=255)                                                                                                                               ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc  <  3:151282/137‑1 (MQ=255)                                                                                                                               ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc  <  2:106197/137‑1 (MQ=255)                                                                                                                               ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc  <  5:271768/137‑1 (MQ=255)                                                                                                                               ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc  <  5:401933/137‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    CGCGGACAACAATCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGAC  >  CP006881/2197210‑2197474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑