Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,349	G→T	G263G (GGC→GGA)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,349	0	G	T	98.9%	279.8 / NA	92	G263G (GGC→GGA)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (38/53);  minor base A (1/0);  total (39/53)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAA  >  CP006881/2197222‑2197482                                                                                                                                |                                                                                                                                      tCGGCATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGACCTGCTGCCTCAAATCCGATGcgcaa                                                                                                                            >  5:385499/1‑135 (MQ=255)     cATCCCTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTcc                                                                                                                        <  5:405785/139‑1 (MQ=255)      atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       >  8:287665/4‑139 (MQ=255)      atcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       <  7:238416/136‑1 (MQ=255)      atcccTGTCGTCATGACGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       <  1:375946/136‑1 (MQ=255)       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      <  5:59107/137‑1 (MQ=255)       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      <  5:412915/137‑1 (MQ=255)       tcccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTATCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      <  5:99702/137‑1 (MQ=255)        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                                                                                                     >  5:16561/2‑139 (MQ=255)        cccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcaca                                                                                                                     >  4:399272/2‑139 (MQ=255)         ccTGTCGTCATGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCGCAAATCCGATGCCGAATTCCacac                                                                                                                    >  2:59021/1‑139 (MQ=255)                  aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                                                                                           >  1:364205/1‑139 (MQ=255)                  aTGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATg                                                                                                           >  8:21503/1‑139 (MQ=255)                   tGCCGCCTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGc                                                                                                          >  8:103443/1‑139 (MQ=255)                    gccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCg                                                                                                         >  7:276400/1‑139 (MQ=255)                     ccgccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGt                                                                                                        >  8:72275/1‑139 (MQ=255)                       gccTTCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                                      <  2:397004/139‑1 (MQ=255)                          ttCCAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAAtt                                                                                                   >  4:343039/1‑139 (MQ=255)                             cAGTCCGCCAAGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGa                                                                                                 <  6:16561/138‑1 (MQ=255)                                  cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       >  3:236886/6‑111 (MQ=255)                                  cgccaaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       <  4:236886/106‑1 (MQ=255)                                     caaGTCTGTTAGTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAAcagc                                                                                        >  2:301238/3‑139 (MQ=255)                                             ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc                                                                                  >  6:445186/4‑137 (MQ=255)                                             ttagTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTACCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACagcggcagc                                                                                  >  3:183935/4‑137 (MQ=255)                                                ggaGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt                                                                             <  7:306941/137‑1 (MQ=255)                                                gTAGCACGGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCt                                                                             <  2:401808/139‑1 (MQ=255)                                                  aGCACGGGTGTATCCTTTTTCATCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCAGATTCCTAAATCCACACCTTTAAATGCGTTAATTTACAGAACAGCGCCAGCAAGCttc                                                                           >  8:267165/1‑138 (MQ=255)                                                   gcacgGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGc                                                                          >  5:117504/4‑139 (MQ=255)                                                   gcacgGGTGTATCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTAAATTGACAGAACAGCGGCAGCAAGCTTg                                                                           <  3:126194/135‑1 (MQ=255)                                                   gcacgGGTGTATCCTTTTTCCTCGCCCCAAATGAGCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTg                                                                           <  5:315636/135‑1 (MQ=255)                                                      cgGGTGTATCCTTTTTCCTCGGCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCtat                                                                       <  6:224281/139‑3 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       <  8:153183/83‑1 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCca                                                                                                                       >  7:153183/1‑83 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               <  6:186762/139‑1 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               <  8:302402/139‑1 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               <  7:103443/139‑1 (MQ=255)                                                              tCCTTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               <  4:163237/139‑1 (MQ=255)                                                                  ttttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                           >  7:188210/1‑139 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               >  8:360060/1‑134 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTg                                                               <  7:360060/134‑1 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCgg                                                            <  6:214659/137‑1 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                           >  6:395109/1‑138 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                           >  1:256022/1‑138 (MQ=255)                                                                   tttCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGt                                                           >  3:202173/1‑138 (MQ=255)                                                                   tttCCTCGTCCCAAAAGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCgg                                                            <  1:89131/137‑1 (MQ=255)                                                                   attCCTCGTCCCAAATGATCTCGTCATGTACTTCCCTTCCATTTAGCCCTGCTGCCTCAAATCCGATGCCGAATTACACAACTATAAAAGCGTTAATTGACAGAACAGAGCCAGAAAGCTTGCTATCCAGCTTGCGGt                                                           >  4:378828/2‑138 (MQ=255)                                                                       cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       <  3:343039/138‑1 (MQ=255)                                                                       cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       <  8:188210/138‑1 (MQ=255)                                                                       cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       <  7:72275/138‑1 (MQ=255)                                                                       cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       <  7:21503/138‑1 (MQ=255)                                                                       cTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACAACTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCCGCTTGCGGTCATa                                                       <  5:402008/138‑1 (MQ=255)                                                                         cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                                    >  1:200164/1‑139 (MQ=255)                                                                         cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                                    >  7:147048/1‑139 (MQ=255)                                                                         cGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                                    >  3:234252/1‑139 (MQ=255)                                                                         cGTCCCAAATGATCTCGTCATGGACTTAGCTTCCATTTCGCCCTGCTGCCTCAAAAACGATGCCGAATTCCACACCTTTAAATGAGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCAAGCTTGCGGTCATAATg                                                    >  4:41984/1‑139 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      <  7:431307/72‑1 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      >  8:431307/1‑72 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                                      >  3:314223/1‑88 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTa                                                                                                      <  4:314223/88‑1 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                                    >  3:73724/1‑138 (MQ=255)                                                                          gTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATACCACACCTATAAATGCGTTAAATGACAGAACAGCCCCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATg                                                    >  6:402008/1‑138 (MQ=255)                                                                           tCCCTAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAACTCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGa                                                  <  5:445186/139‑1 (MQ=255)                                                                           tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       >  5:1501/1‑134 (MQ=255)                                                                           tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATa                                                       <  6:1501/134‑1 (MQ=255)                                                                           tCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGa                                                  <  1:301238/139‑1 (MQ=255)                                                                                aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                             <  8:351700/139‑1 (MQ=255)                                                                                aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                             <  2:216402/139‑1 (MQ=255)                                                                                aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                             <  3:399272/139‑1 (MQ=255)                                                                                      ctcGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGccc                                       >  3:131313/1‑138 (MQ=255)                                                                                         gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                             <  4:24079/130‑1 (MQ=255)                                                                                         gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                             >  3:24079/1‑130 (MQ=255)                                                                                         gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                        <  3:429308/135‑1 (MQ=255)                                                                                                   tCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      >  8:87853/1‑47 (MQ=255)                                                                                                   tCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCcac                                                                                                                      <  7:87853/47‑1 (MQ=255)                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                        >  7:92444/1‑124 (MQ=255)                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                        <  8:92444/124‑1 (MQ=255)                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGc                           <  4:73724/137‑1 (MQ=255)                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                         >  4:438631/1‑139 (MQ=255)                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                         >  1:106197/1‑139 (MQ=255)                                                                                                         ccATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTccc                     <  2:256022/138‑1 (MQ=255)                                                                                                          cATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                    <  4:32975/138‑1 (MQ=255)                                                                                                          cATTTCGCCCTGCTGACTCAAATCCGCTGCAGAATTCCACTCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCATCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                    <  7:267165/138‑1 (MQ=255)                                                                                                               cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa               <  4:183935/132‑1 (MQ=255)                                                                                                               cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa               <  6:117504/132‑1 (MQ=255)                                                                                                                gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt             <  2:364205/134‑1 (MQ=255)                                                                                                                gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt             <  8:276400/134‑1 (MQ=255)                                                                                                                gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt             <  8:337300/134‑1 (MQ=255)                                                                                                                   ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc          <  3:151282/137‑1 (MQ=255)                                                                                                                   ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc          <  2:106197/137‑1 (MQ=255)                                                                                                                   ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc          <  5:271768/137‑1 (MQ=255)                                                                                                                   ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc          <  5:401933/137‑1 (MQ=255)                                                                                                                             aaaTCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTActa  <  4:131313/137‑3 (MQ=255)                                                                                                                                |                                                                                                                                      TCGGCATTCCTGTCGTCATGCCGCCTTCCAGTCCTCCGAGTCTGTTTGTAGCGCGGGTATATCCCTTTTCCTCGTCCCAAATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAA  >  CP006881/2197222‑2197482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑