Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,436	G→A	Y234Y (TAC→TAT)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,436	0	G	A	100.0%	175.4 / NA	56	Y234Y (TAC→TAT)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/34);  total (22/34)

AATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGA  >  CP006881/2197301‑2197571                                                                                                                                        |                                                                                                                                        aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      <  8:351700/139‑1 (MQ=255) aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      <  2:216402/139‑1 (MQ=255) aaTGATCTCGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      <  3:399272/139‑1 (MQ=255)       ctcGTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGccc                                                                                                                                >  3:131313/1‑138 (MQ=255)          gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      <  4:24079/130‑1 (MQ=255)          gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      >  3:24079/1‑130 (MQ=255)          gTCATGGACTTCGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                                                                                                                 <  3:429308/135‑1 (MQ=255)                     cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                                                                                                                 <  8:92444/124‑1 (MQ=255)                     cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGcc                                                                                                                                 >  7:92444/1‑124 (MQ=255)                     cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGc                                                                                                                    <  4:73724/137‑1 (MQ=255)                     cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                                                                                                                  >  1:106197/1‑139 (MQ=255)                     cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                                                                                                                  >  4:438631/1‑139 (MQ=255)                          ccATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTccc                                                                                                              <  2:256022/138‑1 (MQ=255)                           cATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                                                                                                             <  4:32975/138‑1 (MQ=255)                           cATTTCGCCCTGCTGACTCAAATCCGCTGCAGAATTCCACTCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCATCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                                                                                                             <  7:267165/138‑1 (MQ=255)                                cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa                                                                                                        <  6:117504/132‑1 (MQ=255)                                cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa                                                                                                        <  4:183935/132‑1 (MQ=255)                                 gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                      <  2:364205/134‑1 (MQ=255)                                 gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                      <  8:276400/134‑1 (MQ=255)                                 gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                      <  8:337300/134‑1 (MQ=255)                                    ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                   <  3:151282/137‑1 (MQ=255)                                    ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                   <  5:401933/137‑1 (MQ=255)                                    ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                   <  5:271768/137‑1 (MQ=255)                                    ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                   <  2:106197/137‑1 (MQ=255)                                              aaaTCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTActa                                                                                           <  4:131313/137‑3 (MQ=255)                                                 tccGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGCCAATGACTTCTACTATTccg                                                                                      >  1:345883/2‑139 (MQ=255)                                                     aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      >  8:89465/1‑87 (MQ=255)                                                     aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAg                                                                                                                                      <  7:89465/87‑1 (MQ=255)                                                     aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGa                                                                                  <  4:14687/139‑1 (MQ=255)                                                     aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGa                                                                                  >  8:149283/1‑139 (MQ=255)                                                           aaTTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAAt                                                                            <  1:59021/139‑1 (MQ=255)                                                                cacaCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCg                                                                       >  6:170836/1‑139 (MQ=255)                                                                  cacCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGtt                                                                     >  1:383465/1‑139 (MQ=255)                                                                       ttAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCt                                                                                                                          >  4:373659/1‑81 (MQ=255)                                                                       ttAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCt                                                                                                                          <  3:373659/81‑1 (MQ=255)                                                                        tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc                                                                 <  3:74556/137‑1 (MQ=255)                                                                        tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc                                                                 <  3:41984/137‑1 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                   <  3:183427/96‑1 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                        >  6:326869/1‑139 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                        >  7:222200/1‑139 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                        >  8:21415/1‑139 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                        >  5:164864/1‑139 (MQ=255)                                                                               gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCCACAATGAc                                                                                                   >  4:183427/1‑96 (MQ=255)                                                                                         aGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAg                                              >  1:423868/1‑139 (MQ=255)                                                                                           aaCAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGc                                             >  5:255520/1‑138 (MQ=255)                                                                                                cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat                                       >  5:164418/3‑139 (MQ=255)                                                                                                cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat                                       >  5:172034/3‑139 (MQ=255)                                                                                                        aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc                               >  3:84073/1‑139 (MQ=255)                                                                                                        aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc                               >  4:169575/1‑139 (MQ=255)                                                                                                         gCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc                              <  4:84073/139‑1 (MQ=255)                                                                                                         gCTTGCTATCCAGCTTGCGGTCATAATGCACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTAATCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc                              <  6:385499/139‑1 (MQ=255)                                                                                                             gCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGc                           <  7:233716/138‑1 (MQ=255)                                                                                                              ctatCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct                         <  6:255520/136‑3 (MQ=255)                                                                                                                      cttGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCg                 <  5:170836/138‑1 (MQ=255)                                                                                                                             tCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATc          <  7:149283/139‑1 (MQ=255)                                                                                                                                     gACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGga  <  5:395109/139‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AATGATCTCGTCATGGACTTCGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGA  >  CP006881/2197301‑2197571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑