Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,445	A→C	V231V (GTT→GTG)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,445	0	A	C	100.0%	173.6 / NA	54	V231V (GTT→GTG)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/34);  total (20/34)

CGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTC  >  CP006881/2197321‑2197576                                                                                                                             |                                                                                                                                    cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGc                                                                                                                         <  4:73724/137‑1 (MQ=255) cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                                                                                                                       >  1:106197/1‑139 (MQ=255) cGCTTCCATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCAt                                                                                                                       >  4:438631/1‑139 (MQ=255)      ccATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTccc                                                                                                                   <  2:256022/138‑1 (MQ=255)       cATTTCGCCCTGCTGCCTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                                                                                                                  <  4:32975/138‑1 (MQ=255)       cATTTCGCCCTGCTGACTCAAATCCGCTGCAGAATTCCACTCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCATCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCt                                                                                                                  <  7:267165/138‑1 (MQ=255)            cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa                                                                                                             <  4:183935/132‑1 (MQ=255)            cgccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACa                                                                                                             <  6:117504/132‑1 (MQ=255)             gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                           <  2:364205/134‑1 (MQ=255)             gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                           <  8:276400/134‑1 (MQ=255)             gccctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAAt                                                                                                           <  8:337300/134‑1 (MQ=255)                ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                        <  3:151282/137‑1 (MQ=255)                ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                        <  5:401933/137‑1 (MQ=255)                ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                        <  5:271768/137‑1 (MQ=255)                ctgctgccTCAAATCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                        <  2:106197/137‑1 (MQ=255)                          aaaTCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTActa                                                                                                <  4:131313/137‑3 (MQ=255)                             tccGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGCCAATGACTTCTACTATTccg                                                                                           >  1:345883/2‑139 (MQ=255)                                 aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGa                                                                                       <  4:14687/139‑1 (MQ=255)                                 aTGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGa                                                                                       >  8:149283/1‑139 (MQ=255)                                       aaTTCCACACCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAAt                                                                                 <  1:59021/139‑1 (MQ=255)                                            cacaCCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCg                                                                            >  6:170836/1‑139 (MQ=255)                                              cacCTTTAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGtt                                                                          >  1:383465/1‑139 (MQ=255)                                                   ttAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCt                                                                                                                               >  4:373659/1‑81 (MQ=255)                                                   ttAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCt                                                                                                                               <  3:373659/81‑1 (MQ=255)                                                    tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc                                                                      <  3:74556/137‑1 (MQ=255)                                                    tAAATGCGTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGcc                                                                      <  3:41984/137‑1 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGAc                                                                                                        <  3:183427/96‑1 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                             >  8:21415/1‑139 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                             >  5:164864/1‑139 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                             >  7:222200/1‑139 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTtca                                                             >  6:326869/1‑139 (MQ=255)                                                           gTTAATTGACAGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCCACAATGAc                                                                                                        >  4:183427/1‑96 (MQ=255)                                                                     aGAACAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAg                                                   >  1:423868/1‑139 (MQ=255)                                                                       aaCAGCGGCAGCAAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGc                                                  >  5:255520/1‑138 (MQ=255)                                                                            cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat                                            >  5:164418/3‑139 (MQ=255)                                                                            cggcagcaAGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCcatcat                                            >  5:172034/3‑139 (MQ=255)                                                                                    aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc                                    >  4:169575/1‑139 (MQ=255)                                                                                    aGCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGc                                    >  3:84073/1‑139 (MQ=255)                                                                                     gCTTGCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc                                   <  4:84073/139‑1 (MQ=255)                                                                                     gCTTGCTATCCAGCTTGCGGTCATAATGCACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTAATCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGcc                                   <  6:385499/139‑1 (MQ=255)                                                                                         gCTATCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGc                                <  7:233716/138‑1 (MQ=255)                                                                                          ctatCCAGCTTGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct                              <  6:255520/136‑3 (MQ=255)                                                                                                  cttGCGGTCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCg                      <  5:170836/138‑1 (MQ=255)                                                                                                         tCATAATGGACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATc               <  7:149283/139‑1 (MQ=255)                                                                                                                 gACATAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGga       <  5:395109/139‑1 (MQ=255)                                                                                                                   catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct                              <  7:197579/112‑3 (MQ=255)                                                                                                                   catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGcct                              >  8:197579/3‑112 (MQ=255)                                                                                                                   catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGgaga     <  1:7603/137‑1 (MQ=255)                                                                                                                   catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGgaga     <  6:164418/137‑1 (MQ=255)                                                                                                                   catAGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGAAAGgaga     <  8:222200/137‑1 (MQ=255)                                                                                                                      aGCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGAct   >  6:251040/1‑138 (MQ=255)                                                                                                                       gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc  <  5:326869/138‑1 (MQ=255)                                                                                                                       gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc  <  7:287665/138‑1 (MQ=255)                                                                                                                       gCTGCCCACGCCTACCGGCATTCCCTCGACAATGACTTCTACTATTCCGCCGATGGAATCTCCGTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGActc  <  3:423553/138‑1 (MQ=255)                                                                                                                             |                                                                                                                                    CGCTTCCATTTCTTCCGGCTGCTTCAAAGCCGATGCCGAATTCCACACCTTTAAATGCGTTAATTGACAGAACAGCAGCAGCAAGCTTGCTGTCCAGTTTGCGGTCATAATGGACGTAGCTGCCAACGCCTACTGGCATTCCCTCGACAATGACTTCTACAATTCCGCCGATGGAATCTCCGTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTC  >  CP006881/2197321‑2197576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑