Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,634	T→A	A168A (GCA→GCT)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,634	0	T	A	100.0%	213.8 / NA	68	A168A (GCA→GCT)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (45/23);  total (45/23)

GTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACG  >  CP006881/2197502‑2197768                                                                                                                                     |                                                                                                                                       gTTTGCCTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCa                                                                                                                                  <  4:54023/139‑1 (MQ=255)       cTTTGCTTCATCAATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGc                                                                                                                            <  3:169575/139‑1 (MQ=255)                atcaATGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTgg                                                                                                                   >  7:3946/1‑138 (MQ=255)                     tttcatccATCTTTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATATGCAACACGTGGCCCGc                                                                                                              <  1:424370/133‑1 (MQ=255)                     tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                              <  5:251040/139‑1 (MQ=255)                     tGGCAGCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                              <  6:357860/139‑1 (MQ=255)                          gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCt                                                                                                         <  1:194088/139‑1 (MQ=255)                          gCCATCATTTTTTTGCCTGCCTCTTCATCGTAGCATCTGACAGGAGACTCTTCCGGTAAACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCt                                                                                                         >  6:212272/1‑139 (MQ=255)                                              ctcttcATCGAAGCTTCTGACAGGTGACTCTTCCGTTACACGCTGCATGGCTTCAATTGCCTTATACTCTGTTTTTTCAGCTTTAACAGCACCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCagaaaga                                                                                     <  4:202173/138‑1 (MQ=255)                                                        tAGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAg                                                                           >  4:364041/1‑139 (MQ=255)                                                         aGCATCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGc                                                                          >  7:429960/1‑139 (MQ=255)                                                         aGCATATGACAGGATACTCTTCATTAACAAGCAGCAAGTCTTCAAATGACGTATACTCTCTATTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAATGCCAAGCTCAGAAAGAATCtttt                                                                              >  6:90409/1‑135 (MQ=255)                                                             tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                                                        >  5:279050/1‑135 (MQ=255)                                                             tCTGACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGcta                                                                        >  3:56044/1‑135 (MQ=255)                                                                gACAGGAGACTCTTCCGTTACCCGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCAAATTTGCAACACGTGGCCAGCCACCTTAATGACAAGCTAAGAAAGAATCTTTTTAGCTACatcc                                                                   >  8:22065/1‑136 (MQ=255)                                                                gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                   >  8:74225/1‑138 (MQ=255)                                                                gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                   >  5:439260/1‑138 (MQ=255)                                                                gACAGGAGACTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcc                                                                   >  8:15602/1‑138 (MQ=255)                                                                  caggAGACTCTTCCGTTACACGCTGCAAGTCTTCAAATGACGTATACTCTGTATATTCAGCAATAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGcccc                                                                 <  3:142479/137‑1 (MQ=255)                                                                     gagaCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCGTAATGCCAAGCTCAGAAAGAATCTTGTTAGCTACAGCCCCTgc                                                              >  8:169235/1‑139 (MQ=255)                                                                        aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                                                           >  7:180844/1‑139 (MQ=255)                                                                        aCTCTTCCGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGAAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTgccgc                                                           >  4:134128/1‑139 (MQ=255)                                                                            ttccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAct                                                       >  1:181724/2‑139 (MQ=255)                                                                              ccGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCAc                                                        <  3:364041/136‑1 (MQ=255)                                                                               cGTTACACGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCActct                                                     <  7:74225/138‑1 (MQ=255)                                                                                      cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                              <  1:439951/74‑1 (MQ=255)                                                                                      cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGc                                                                                                              >  2:439951/1‑74 (MQ=255)                                                                                      cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg                                             >  3:184275/1‑139 (MQ=255)                                                                                      cGCTGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAgttg                                             >  6:218238/1‑139 (MQ=255)                                                                                         tGCAAGTCTTCAATTGACGTTTACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCACGCCACCTTAAGGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCAACGCTGCCAgttgtt                                           >  8:278346/1‑138 (MQ=255)                                                                                         tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCCCTCTGACAGTTGttt                                          >  4:144643/1‑139 (MQ=255)                                                                                         tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt                                          >  2:49567/1‑139 (MQ=255)                                                                                         tGCAAGTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGttt                                          >  2:28854/1‑139 (MQ=255)                                                                                           caagTCTTCAATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTcc                                        >  2:80139/4‑139 (MQ=255)                                                                                                 ttcaATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCt                                  >  7:268256/2‑139 (MQ=255)                                                                                                 ttcaATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCt                                  >  2:321058/2‑139 (MQ=255)                                                                                                   caATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTa                                                                                                       >  6:258670/1‑68 (MQ=255)                                                                                                   caATTGACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTa                                                                                                       <  5:258670/68‑1 (MQ=255)                                                                                                        gACGTATACTCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                            <  1:28854/138‑2 (MQ=255)                                                                                                              tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCt                                                                                             <  5:59307/64‑1 (MQ=255)                                                                                                              tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCt                                                                                             >  6:59307/4‑67 (MQ=255)                                                                                                              tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                            >  5:38429/4‑131 (MQ=255)                                                                                                              tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAagaa                            <  6:38429/129‑2 (MQ=255)                                                                                                              tactCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCaa                     >  1:200984/4‑139 (MQ=255)                                                                                                               actCTGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                    <  2:181724/137‑1 (MQ=255)                                                                                                                   tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCTAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                >  2:23008/1‑139 (MQ=255)                                                                                                                   tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                >  5:444032/1‑139 (MQ=255)                                                                                                                   tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGcaca                >  2:357595/1‑139 (MQ=255)                                                                                                                   tGTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGca                  <  8:3946/137‑1 (MQ=255)                                                                                                                    gTTTTTTCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAt               >  4:41350/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  8:368578/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  7:403465/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  8:156886/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  6:290697/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  4:397749/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  6:140608/1‑139 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAt               >  2:319475/1‑138 (MQ=255)                                                                                                                     ttttttCAGCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCATGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACAtt              >  5:249005/1‑139 (MQ=255)                                                                                                                             gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                    <  6:183961/125‑1 (MQ=255)                                                                                                                             gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAg                    >  5:183961/1‑125 (MQ=255)                                                                                                                             gCTTTAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCatat      <  4:184275/139‑1 (MQ=255)                                                                                                                             gCTTTAACAGCGCCAATTTGCAACACGTGGACCGCCACCTTAAAGCCAAGCTCAGAAAGAATCTTATTAGCTAAAGCCCCTGCCGCCACTCTGACATTAGTTTACCTTGCTGAAGAACACTCAAGCACAGTTAACata       >  6:354610/1‑138 (MQ=255)                                                                                                                                 tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc   <  8:403465/138‑1 (MQ=255)                                                                                                                                 tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc   >  1:147105/1‑138 (MQ=255)                                                                                                                                 tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc   <  8:268256/138‑1 (MQ=255)                                                                                                                                 tAACAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCAc   >  3:222091/1‑138 (MQ=255)                                                                                                                                  aaCAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACg  <  5:140608/138‑1 (MQ=255)                                                                                                                                  aaCAGCGCCAATTTGCAACACGTGGCCCGCCACCTTAATGCCAAGCTCAGAAAGAATCTTTTTAGCTACAGCCCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAACGCTCAAGCACATTTCTCATATCACg  <  3:41350/138‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GTTGGCCTTCGCTTCATCAATGGCAGCCATCATTTTTTTGCCCGCTTCTTCATCGTAGCATCTGACCGGAGACTCCTCCGTTACACGCTGCAGGTCATCAATTGACGTATATTCTGTTTTTTCAGCTTTAACTGCGCCAATTTGCAATACGTGACCTGCCACCTTAATGCCGAGCTCAGAAAGAATCTTTTTAGCGACAGCTCCTGCCGCCACTCTGACAGTTGTTTCCCTTGCTGAAGAGCGCTCAAGCACATTTCTCATATCACG  >  CP006881/2197502‑2197768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑