Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,077	C→G	A283A (GCG→GCC)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,077	0	C	G	100.0%	65.2 / NA	21	A283A (GCG→GCC)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (11/10);  total (11/10)

TTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAA  >  CP006881/2173951‑2174200                                                                                                                               |                                                                                                                            cttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgat                                                                                                                  <  7:2406/134‑1 (MQ=255)  ttttcatcCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTtgatca                                                                                                                <  6:259403/136‑1 (MQ=255)                            aaGCTGCGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTc                                                                                      <  2:331649/139‑1 (MQ=255)                                  cGATATTTTGGCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGaa                                                                                 >  1:359579/1‑138 (MQ=255)                                            gCCGCTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGgcgcgt                                                                      >  1:42345/1‑137 (MQ=255)                                                cTGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCttt                                                                  <  2:42345/139‑1 (MQ=255)                                                 tGAGAGCAGTGCTTTTTTCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTc                                                                 >  3:440333/1‑139 (MQ=255)                                                          tgcttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtg                                                        >  4:305824/4‑138 (MQ=255)                                                            cttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc                                                      <  3:305824/138‑4 (MQ=255)                                                            cgattttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc                                                      <  8:197962/136‑4 (MQ=255)                                                                 ttcccAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATAGTCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAgg                                                  <  4:179756/136‑2 (MQ=255)                                                                      agCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAAt                                             >  3:386818/2‑138 (MQ=255)                                                                           tGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                          >  4:439254/1‑136 (MQ=255)                                                                             tACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAgag                                     <  6:350233/139‑3 (MQ=255)                                                                                       ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAg                           >  7:166910/5‑139 (MQ=255)                                                                                       ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTAAAAAATATTACAAGGTTAATTAAAAGAGCAGCCCaaac                           >  2:359990/5‑138 (MQ=255)                                                                                         gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                          >  1:104026/3‑122 (MQ=255)                                                                                         gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                          <  2:104026/120‑1 (MQ=255)                                                                                              aaGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTGTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCa                    >  5:100129/1‑139 (MQ=255)                                                                                                                        tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag  <  2:116458/131‑4 (MQ=255)                                                                                                                        tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTGCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag  >  1:116458/1‑128 (MQ=255)                                                                                                                               |                                                                                                                            TTCATCATCCAGTTTTTCGTTTTGCCGAAGCTGCGATATTTTGGCCGCTGAAAGAAGTGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAA  >  CP006881/2173951‑2174200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑