Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,197,871	A→G	G89G (GGT→GGC)	U712_11010 ←	Chorismate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,197,871	0	A	G	100.0%	170.8 / NA	54	G89G (GGT→GGC)	U712_11010	Chorismate synthase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/29);  total (25/29)

CGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTCCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGAGAGCAATTGGTGAACCGAGTGTTCGTGCATGACGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCG  >  CP006881/2197743‑2198003                                                                                                                                 |                                                                                                                                     cgcTCAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTACTTTTGTTCATATTCTGTTATCGTAGCTGCGCCCATGAttt                                                                                                                            >  7:322929/1‑139 (MQ=255)     cAAGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGt                                                                                                                        <  1:321058/139‑1 (MQ=255)      aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTg                                                                                                                         <  8:180844/137‑1 (MQ=255)      aaGCACATTTCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTc                                                                                                                       <  7:56492/139‑1 (MQ=255)         cacaTTTCTCCTATAAAGATGATTATATTTATTTGCAACGTTTAAGTCATAGTGCCCATGTCTCGGTCTTGAAAGCTGGAGCTTCATTTCCTTTTCTTCATATGCTGTAATCGGATCGGCGCCCATGATTTTTGTCCaa                                                                                                                    <  7:278346/139‑1 (MQ=255)              ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCAATTACTTTTCTTAATATTATGTAATAGGAGCGGCGCCCCATATTTTTGTCCTATgat                                                                                                                >  4:77790/1‑136 (MQ=255)              ttCTCATATCACGATGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCGACTCTTCCTTTTCGTGATCATCTGTAATCGGAGCGGCGCCCCATATTTTTTTCCAAGGCt                                                                                                                >  5:425864/1‑138 (MQ=255)                           atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTGCTTTTGTTCATCTTGTGTAATCGGAGCGGCGCCCATGTTTTTTGTGCAATGGTTGCAATCGTTAttt                                                                                                  >  4:168391/2‑139 (MQ=255)                           atGATTATATTTAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTAATTTCCTTTTCTTCGTCTTCTGTTATCGGAGCGGCGCCCATGATTTTTGTAGATTGCTTACAATCGTTAttt                                                                                                  >  8:75501/2‑139 (MQ=255)                                    tttAATTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTGTTCATCTTCTGTTATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCCATCGTTTTTTTCAactacg                                                                                         >  5:75066/1‑139 (MQ=255)                                       aaTTGCACCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTTTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAActacga                                                                                        >  6:314458/1‑137 (MQ=255)                                            caccGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATATTCTGTAATCGGAGCGGGGCCCATGATTTTTGTGGAATTCGTGCAAACGTTTTTTTAAACTTCGACTGCAAt                                                                                 >  2:76207/3‑139 (MQ=255)                                               cGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTgg                                                                              <  5:263872/139‑1 (MQ=255)                                               cGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTgg                                                                              <  4:183477/139‑1 (MQ=255)                                                 tttAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCATTTCTTCATCATCTGTAATAGGAGCGGCGCCCAAGATTTTTTTGCAATGCTTCCAATCCTTATTTTCAACAACTAGAGCAATTggc                                                                             >  6:186526/1‑137 (MQ=255)                                                   tAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCaa                                                                                                           <  8:288095/106‑1 (MQ=255)                                                   tAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCaa                                                                                                           >  7:288095/1‑106 (MQ=255)                                                    aaGTCAGCGTGCCCAGGTCGCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGTGaa                                                                          <  6:249005/138‑1 (MQ=255)                                                    aaGGCAGCGGGCCCAGGTCTCGGGCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGaa                                                                          <  6:75066/138‑1 (MQ=255)                                                     aggcAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        <  6:444032/136‑1 (MQ=255)                                                     aGTGAGCGTGCCCAGGTCTCGGTCAGGAAATCTGGCGCTTCTTTTCCTTTTCTTCCTCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        <  4:360783/139‑1 (MQ=255)                                                     aGTCTGCGTGCCCAGGGCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTGCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCTATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        <  8:52533/139‑1 (MQ=255)                                                     aGTCAGCGTGCCCAGGTCTCGGGCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        <  7:269881/139‑1 (MQ=255)                                                      gTCAGCGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCGTCATTTCCTTTTCTTCATCTTATGTTATCGGAGCGGCGCCCAAGATTTTTGTCCAATGCTTCGAATAGTTATTTTAAACTACGAGGGCAATTGGAGAACCg                                                                       >  8:239831/1‑139 (MQ=255)                                                       gacaggTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGa                                                                      <  3:274562/134‑1 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTGATTTCCTTTTCTTCGTCTTCTGTAATCGGTGCGGCGGCCATGTTTTTTGTGCAATGGTTCCAAACGTTATTTTCAAATAGGAGAGCAATTGGCGAGCCGGgtgt                                                                  >  3:26423/1‑139 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTGATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCCTGATTTTTTTCCACTGCTTCCAATCGTTTTTTTGAACTACGAGAGCAATTGGCGCACCGAgtg                                                                   >  3:43522/1‑138 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTGTTCATCTTCTGTTATCGGAGCGGCGCCCCTGTTTTTTGTCCAATGCTTCCAATCGTTATTTTGAACTACGAGAGCAATTGGCGGACCGAggg                                                                   >  3:278188/1‑136 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTGATCTTCTGTAATCGGAGCGGCGCCCATGGTTTTTGTGCAATGCTTCCAATCGTTTTTTTGAACAAAGAGAGCAATTGGCGAAACGAgtg                                                                   >  1:82333/1‑138 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCg                                                                                                        >  7:408648/1‑101 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCg                                                                                                        <  8:408648/101‑1 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAgtgt                                                                  <  7:15602/139‑1 (MQ=255)                                                           cGTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATATTCTTTAATCCGAGCGGCGCCCCATATTTTTGTGCAAAGATTCCAATCGTTAATTTAAACAACGACACCAAATGGCGAAACGAgtg                                                                   >  5:71953/1‑138 (MQ=255)                                                           cGTGCCCAGGTCTCGGGCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTACTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAgtgt                                                                  <  3:134128/139‑1 (MQ=255)                                                           cGTGCCCAGGGCTCGGGCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGACCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAgtgt                                                                  <  7:22065/139‑1 (MQ=255)                                                            gTGCCCAGGTCTCGGTCTGGAAATCTGGCGCTTCAATTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAgtgta                                                                 <  2:182076/139‑2 (MQ=255)                                                                  aGGTCTCGGTCTGGAAATCTTGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCa                                                           <  5:186526/139‑1 (MQ=255)                                                                         ggTCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAAATACGATAGCAATTTGCGAACCGAGTGTACGTTCCTGGCGCa                                                    >  7:423394/1‑139 (MQ=255)                                                                         ggTCTGGAAATCTGGCGCTTCAGTTCCTTTTAGTGATCTTCTGTAATCGGAGAGGCGCCCATGATTTTTGTCAAATGCGTACAATCGTTATTTTCAACTACGATCGGAATTGGCGCCCCGAGTGTACGTGCCTGGCGCa                                                    >  6:174228/1‑139 (MQ=255)                                                                           tCTGGAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCAcc                                                  <  3:68683/139‑1 (MQ=255)                                                                              ggAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        <  3:270059/114‑1 (MQ=255)                                                                              ggAAATCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAAcc                                                                        >  4:270059/1‑114 (MQ=255)                                                                                 aaTCTGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTc                                            <  4:341121/139‑1 (MQ=255)                                                                                  atctGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCa                                           <  5:398283/136‑1 (MQ=255)                                                                                  atctGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCa                                           <  3:144643/136‑1 (MQ=255)                                                                                     tGGCGCTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATaa                                        >  5:419096/1‑139 (MQ=255)                                                                                       gcgcTTCATTTGCTTTTGTTGATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTGCCATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTTGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAAtt                                      >  8:301247/1‑139 (MQ=255)                                                                                       gcgcTTCATTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAAtt                                      <  6:259902/139‑1 (MQ=255)                                                                                              aTTTCCTTTTCTTCATCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGcc                                <  6:223694/138‑1 (MQ=255)                                                                                                            aTCTTCTGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGAt                  <  1:177708/138‑1 (MQ=255)                                                                                                              cttctGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTg               >  3:284585/3‑139 (MQ=255)                                                                                                                  tGTAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGGCTGGTCGTTTTCGATCTGCATg           >  2:127216/1‑139 (MQ=255)                                                                                                                    tAATCGGAGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATgcg         <  1:23008/139‑1 (MQ=255)                                                                                                                           aGCGGCGCCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACTACGAGAGCAATTGGCGAACCGAGTGTACGTGCATGGCGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGgcggccg  >  3:400046/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                     CGCTCAAGCACATTTCTCATATCACGGTGATTATATTTAATCGCTCCGTTTAAGTCAGCGTGCCCAGGTCTCGGTCTGGAAATTTGGCGCTTCATTTCCTTTTCTTCATCCTCTGTAATCGGAGCCGCACCCATGATTTTTGTCCAATGCTTCCAATCGTTATTTTCAACGACGAGAGCAATTGGTGAACCGAGTGTTCGTGCATGACGCACCCCGCTCATAATTTTGGCCTGGTCTTTTTCGATCTGCATGCGGCGGCCG  >  CP006881/2197743‑2198003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑