Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,198,263	C→G	P240P (CCG→CCC)	U712_11015 ←	Chemotaxis protein methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,198,263	0	C	G	100.0%	195.7 / NA	60	P240P (CCG→CCC)	U712_11015	Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (31/29);  total (31/29)

TCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAAACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTC  >  CP006881/2198132‑2198397                                                                                                                                    |                                                                                                                                       tctcATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTg                                                                                                                                  <  4:379362/138‑1 (MQ=255)    cATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTg                                                                                                                                  >  2:134173/1‑135 (MQ=255)            cttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCt                                                                                                                        <  2:178772/137‑1 (MQ=255)            cttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCg                                                                                                                      >  8:239346/1‑139 (MQ=255)            cttctCCATTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCt                                                                                                                        <  5:28367/137‑1 (MQ=255)             ttctCCCTTCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCgg                                                                                                                     >  6:313704/1‑139 (MQ=255)                    ttCATCGCATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGcc                                                                                                              >  7:154119/1‑139 (MQ=255)                           cATTAACGCTTTTAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaa                                                                                                       <  7:282002/139‑2 (MQ=255)                                      ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            >  5:153629/1‑139 (MQ=255)                                      ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            >  1:433449/1‑139 (MQ=255)                                      ttAAGTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            >  2:29975/1‑139 (MQ=255)                                       taagtaagTCTATTTTAACATATTTTTCAGAATAAAGTCAGTATTTCTTTTTTGATAAAAGAATGTAGCGGCTGGGTCTAGGCCATATTTCTGGGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGAACTGCAtt                                                                                            <  5:200524/138‑1 (MQ=255)                                       taagtaagACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            <  1:231756/138‑1 (MQ=255)                                       taagtaagACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            <  8:154119/138‑1 (MQ=255)                                       tAATTAAGACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAtt                                                                                            <  8:357518/138‑1 (MQ=255)                                             agactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTcttctt                                                                                      >  5:412621/3‑138 (MQ=255)                                              gactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCAttcttcttc                                                                                     >  1:105339/2‑138 (MQ=255)                                              gactactATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCa                                                                                    <  5:364503/138‑1 (MQ=255)                                                   ctaTAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAgaa                                                                                                   >  2:358282/1‑117 (MQ=255)                                                   ctaTAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAgaa                                                                                                   <  1:358282/119‑3 (MQ=255)                                                     ataACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTAtg                                                                             >  3:118983/1‑139 (MQ=255)                                                         cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc                                                                          >  5:380173/1‑138 (MQ=255)                                                         cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc                                                                          >  8:43251/1‑138 (MQ=255)                                                         cATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGc                                                                          >  1:81286/1‑138 (MQ=255)                                                          atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa                                                                        <  8:326692/139‑1 (MQ=255)                                                          atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa                                                                        <  7:343875/139‑1 (MQ=255)                                                          atatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCa                                                                        <  7:243000/139‑1 (MQ=255)                                                           tatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAt                                                                       >  1:26750/1‑139 (MQ=255)                                                           tatTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAt                                                                       >  8:112268/1‑139 (MQ=255)                                                              ttttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAtttt                                                                    >  1:347858/1‑139 (MQ=255)                                                               tttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaaa                                                                                                      >  4:268857/1‑102 (MQ=255)                                                               tttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACaaaaa                                                                                                      <  3:268857/104‑3 (MQ=255)                                                                ttCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCAttttt                                                                   <  6:380173/138‑1 (MQ=255)                                                                   aGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTtaaa                                                                <  5:103692/138‑1 (MQ=255)                                                                   aGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGATCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTtaaa                                                                >  6:103692/1‑138 (MQ=255)                                                                          agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                        <  1:134173/139‑1 (MQ=255)                                                                          agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                        <  6:368623/139‑1 (MQ=255)                                                                          agtcagtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGctc                                                        <  5:313704/139‑1 (MQ=255)                                                                               gtcTATCTTTTTTGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCt                                                   >  1:406119/1‑139 (MQ=255)                                                                                        ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt                                            <  4:118983/137‑1 (MQ=255)                                                                                        ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCt                                            <  3:168790/137‑1 (MQ=255)                                                                                        ttttGATAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTATCGGGATTGAAGATTTGCTCGGTGCTGCCCACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCttt                                          >  7:123737/1‑139 (MQ=255)                                                                                             aTAAAAGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTa                                     <  6:412621/139‑1 (MQ=255)                                                                                                aaaaaaTGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                  <  6:177379/139‑1 (MQ=255)                                                                                                aaaGAATGTATCGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                  <  6:153629/139‑1 (MQ=255)                                                                                                aaaGAATGTATAGGCTGGGACTAGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                  <  2:433449/139‑1 (MQ=255)                                                                                                                  gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                  >  5:366412/5‑121 (MQ=255)                                                                                                                  gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTaaaa                                  <  6:366412/117‑1 (MQ=255)                                                                                                                  gactaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCgg                >  3:376529/5‑139 (MQ=255)                                                                                                                    ctaGGCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGca              <  1:29975/137‑1 (MQ=255)                                                                                                                       ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac           >  7:201869/1‑139 (MQ=255)                                                                                                                       ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac           >  4:115067/1‑139 (MQ=255)                                                                                                                       ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcac             <  7:43251/137‑1 (MQ=255)                                                                                                                       ggCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCACTTCCTTCGCGCTTTCTGTAAAATAAATAAAAACATTCCGGcacac           >  2:252732/1‑139 (MQ=255)                                                                                                                        gCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAATTACATTGCGGcacac           >  4:31594/1‑138 (MQ=255)                                                                                                                        gCCAAATTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGcacac           >  2:253269/1‑138 (MQ=255)                                                                                                                            aaTTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCa                                                                                    <  1:276758/61‑1 (MQ=255)                                                                                                                            aaTTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCa                                                                                    >  2:276758/1‑61 (MQ=255)                                                                                                                            aaTTTCTCGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGAtt       >  5:108278/1‑138 (MQ=255)                                                                                                                                 ctcGGGATTGAAGATTTGCTCGGTGCTGCCGACAAAAAGAACTACATTCTTCTTCAAGCTATGTGCCAGTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTc  >  7:8559/1‑138 (MQ=255)                                                                                                                                    |                                                                                                                                       TCTCATGACTTCTTCTCCCTTCATCGCATTAACGCTTTTAAGTAAAACTACTATAACATATTTTTCAGCAAAAAGTCAGTCTATCTTTTTTGATAAAAGAATGTATCGGCTGGAGCCAGGCCAAATTTCTCCGGATTGAAGATTTGCTCGGTGCTGCCGACAAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTC  >  CP006881/2198132‑2198397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑