Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,198,427	G→A	L186M (CTA→TTG)	U712_11015 ←	Chemotaxis protein methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,198,427	0	G	A	100.0%	179.3 / NA	57	L186M (CTA→TTG)	U712_11015	Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (36/21);  total (36/21)

AAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGA  >  CP006881/2198293‑2198555                                                                                                                                       |                                                                                                                                 aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                              >  1:151721/1‑139 (MQ=255) aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                              >  7:137227/1‑139 (MQ=255) aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                              >  3:111693/1‑139 (MQ=255) aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATtg                                                                                                                              >  2:442834/1‑139 (MQ=255) aaaaAGAACTCCATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTAATAACGATCCGCCAGCAAATtg                                                                                                                              >  5:254012/1‑139 (MQ=255) aaaaAGAACTCCAATCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTACTTCGCGCTTTATGTAAAATAAATAAATACATAGAGGCACAAGATTAAGTAAAACTCTTGTTCAAAAAGATCCGCCAGCAAATtg                                                                                                                              >  7:2940/1‑139 (MQ=255)            cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACTCGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                                                                                                    <  8:123737/138‑1 (MQ=255)            cATTCTTCTTCAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGa                                                                                                                    <  8:201869/138‑1 (MQ=255)                    atCAAGCTATGTGCGATTTTTTAATACCGCGCTTCCTTGGCGCTTTCTGTTAAATAATTAATTTCATTGCGGCACAGGATTAAGGCTAAGTCTAGTTCTTAAAGATCCGCCAGAAAATTTTGATTATTGAATGtattat                                                                                                           >  2:345789/2‑139 (MQ=255)                      cAAGCTATGTGCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTc                                                                                                         >  8:254042/1‑139 (MQ=255)                             tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa                                                                                                  >  3:71555/1‑139 (MQ=255)                             tgtgCCATTTTTAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGa                                                                                                  >  5:19195/1‑139 (MQ=255)                                     ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGTCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt                                                                                          >  6:68061/1‑137 (MQ=255)                                     ttttAAATACAGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCgtt                                                                                          >  8:306419/1‑137 (MQ=255)                                        taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                        <  7:306419/138‑1 (MQ=255)                                        taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                        <  1:442834/138‑1 (MQ=255)                                        taaatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                        <  8:2940/138‑1 (MQ=255)                                        taaatacaGCTCTTCCTTCGCGCCTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                        <  5:114764/138‑1 (MQ=255)                                        taaatacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGGTTTTTGAATGTAATATTCTTTTTGATTTCCGtttt                                                                                        <  1:252732/138‑1 (MQ=255)                                          aatacaGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGttttca                                                                                      <  4:71555/138‑3 (MQ=255)                                            tacaGCTCTTACTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACtt                                                                                   <  2:81286/136‑1 (MQ=255)                                               aGCTCTTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCAt                                                                                <  2:406119/139‑1 (MQ=255)                                                 ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATaa                                                                              >  4:71972/1‑139 (MQ=255)                                                 ctctTCCTTCGCGCTTTCTGTAAAATAAATAAATAAATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTATTTAATGTAATATTCTTTTTGATATCCGTTTTCACTTCATa                                                                               >  5:243376/1‑138 (MQ=255)                                                    ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAAc                                                                             <  6:108278/137‑1 (MQ=255)                                                    ttCCTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACtt                                                                           >  4:57383/1‑139 (MQ=255)                                                      ccTTCGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTc                                                                          >  5:223715/1‑138 (MQ=255)                                                                 tCTGTAAAATAAATAAATACATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGtttt                                                              >  3:337826/1‑139 (MQ=255)                                                                            aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                                   <  6:223715/139‑1 (MQ=255)                                                                            aataaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATaa                                                   <  3:71972/139‑1 (MQ=255)                                                                              taaataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACg                                                 <  7:346063/139‑1 (MQ=255)                                                                                aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                               <  4:305137/139‑1 (MQ=255)                                                                                aataCATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGAt                                               <  8:137227/139‑1 (MQ=255)                                                                                    cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGCTCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                           >  8:103588/1‑139 (MQ=255)                                                                                    cATTGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCttt                                           <  2:60363/139‑1 (MQ=255)                                                                                       tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                         >  5:71533/1‑138 (MQ=255)                                                                                       tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                         >  8:169309/1‑138 (MQ=255)                                                                                       tGCGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                         >  3:60538/1‑138 (MQ=255)                                                                                       tGCGGCACACGATTAAGTCAAAGGCTTGTTCATAACGATCCGCCAGCAAATTGTGATTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                         >  4:72123/1‑138 (MQ=255)                                                                                         cGGCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTca                                         >  3:98203/1‑136 (MQ=255)                                                                                          ggCACACGATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGa                                     <  4:337826/139‑1 (MQ=255)                                                                                            cacacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATAGTGCTTTTTCAATGTATTATTCTTTTTGATTTCCGTTTTCACTTCATACCTACTATTTGCGTTTTGCG‑AAAATAACGATCTTTCACAGAtag                                  >  3:276149/1‑137 (MQ=255)                                                                                              cacgATTAAGTCAAAGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagc                                 >  6:25971/1‑136 (MQ=255)                                                                                                           aaGTCTTGTTCATAACGATCCGCCAGCAATTTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTATTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTga                     >  1:365270/1‑137 (MQ=255)                                                                                                           aaGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGCTCTTTCACAGATAGCGGCCCTTCCTGt                     >  5:282011/1‑138 (MQ=255)                                                                                                                 tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc              >  3:65381/1‑139 (MQ=255)                                                                                                                 tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc              >  8:4063/1‑139 (MQ=255)                                                                                                                  gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                               <  3:98381/121‑6 (MQ=255)                                                                                                                  gTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAtagcgg                               >  4:98381/1‑116 (MQ=255)                                                                                                                   ttCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCAATTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAccg             <  7:405160/138‑2 (MQ=255)                                                                                                                       tAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt        >  1:321569/1‑139 (MQ=255)                                                                                                                        aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCTTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACAGCTCCt        >  8:372215/1‑138 (MQ=255)                                                                                                                        aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt        >  4:296291/1‑138 (MQ=255)                                                                                                                             tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAta                                   <  6:134718/103‑2 (MQ=255)                                                                                                                             tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGAta                                   >  5:134718/4‑105 (MQ=255)                                                                                                                             tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCTCTTCCTGTAAAGACCGCTCCTGATaaa  >  2:168428/4‑137 (MQ=255)                                                                                                                             tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATaaa  >  5:89194/4‑137 (MQ=255)                                                                                                                                       |                                                                                                                                 AAATAGGACTCCATTCTTTTTCAAGCTATGTGCCATTTTTAAATAAAGCTCTTCCTTGGCGCTTTCTGTAAAATAAATAAATACATTGCGGCACACAATTAAGTCAAAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGA  >  CP006881/2198293‑2198555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑