Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,527 T→C V152V (GTA→GTG)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,5270TC100.0% 153.0 / NA 48V152V (GTA→GTG) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/28);  total (20/28)

AAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAA  >  CP006881/2198399‑2198651
                                                                                                                                |                                                                                                                            
aaGTCTTGTTCATAACGATCCGCCAGCAATTTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTATTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTga                                                                                                                     >  1:365270/1‑137 (MQ=255)
aaGTCTTGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGCTCTTTCACAGATAGCGGCCCTTCCTGt                                                                                                                     >  5:282011/1‑138 (MQ=255)
      tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc                                                                                                              >  3:65381/1‑139 (MQ=255)
      tGTTCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAcc                                                                                                              >  8:4063/1‑139 (MQ=255)
        ttCATAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCAATTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGAccg                                                                                                             <  7:405160/138‑2 (MQ=255)
            tAACGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt                                                                                                        >  1:321569/1‑139 (MQ=255)
             aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCTTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACAGCTCCt                                                                                                        >  8:372215/1‑138 (MQ=255)
             aaCGATCCGCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCt                                                                                                        >  4:296291/1‑138 (MQ=255)
                  tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCTCTTCCTGTAAAGACCGCTCCTGATaaa                                                                                                  >  2:168428/4‑137 (MQ=255)
                  tccgCCAGCAAATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATaaa                                                                                                  >  5:89194/4‑137 (MQ=255)
                                 tgCTTTTTGAATGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                                                                    <  2:133478/138‑1 (MQ=255)
                                           aTGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                                                                    >  8:217974/1‑128 (MQ=255)
                                           aTGTAATATTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCt                                                                                    <  7:217974/128‑1 (MQ=255)
                                                atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                                                                    <  2:151721/139‑1 (MQ=255)
                                                atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                                                                    <  6:254012/139‑1 (MQ=255)
                                                atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCtttt                                                                     <  4:376529/138‑1 (MQ=255)
                                                   ttCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTtcat                                                                 <  6:89194/139‑1 (MQ=255)
                                                                ccGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGc                                                    >  4:331592/1‑139 (MQ=255)
                                                                 cgttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGc                                                    <  3:57383/135‑1 (MQ=255)
                                                                  gttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGcta                                                  <  5:25971/137‑3 (MQ=255)
                                                                  gttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGcta                                                  <  7:103588/137‑3 (MQ=255)
                                                                            cATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGa                                          <  3:331592/137‑1 (MQ=255)
                                                                             aTAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg                                       <  7:254042/139‑1 (MQ=255)
                                                                             aTAACTTCTATTTGCGTTATGCGTAATATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAATACCGCTCCTGATAATCGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg                                       <  2:365270/139‑1 (MQ=255)
                                                                                    cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata                                        <  4:315547/131‑3 (MQ=255)
                                                                                    cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata                                        >  3:315547/1‑129 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                <  6:19195/136‑1 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                <  5:68061/136‑1 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                <  1:347301/136‑1 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                <  5:380305/136‑1 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                <  3:296291/136‑1 (MQ=255)
                                                                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAc                             >  2:51100/1‑139 (MQ=255)
                                                                                          gcgttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAcct                           <  4:98203/135‑3 (MQ=255)
                                                                                            gttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACctttt                        >  7:140764/2‑139 (MQ=255)
                                                                                                ttgcgaaaaTAACGATCATTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTATCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGc                     <  4:276149/133‑1 (MQ=255)
                                                                                                 gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                    <  8:140764/136‑1 (MQ=255)
                                                                                                 gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                    <  7:4063/136‑1 (MQ=255)
                                                                                                            cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa        >  2:249941/1‑139 (MQ=255)
                                                                                                            cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa        >  7:123313/1‑139 (MQ=255)
                                                                                                              aTCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATg      >  5:150741/1‑139 (MQ=255)
                                                                                                                 tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGATGACCTTTTTGCTGGTCCAAAAGCATGGc    >  6:413373/2‑138 (MQ=255)
                                                                                                                 tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc    >  4:438552/2‑138 (MQ=255)
                                                                                                                  ttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc    >  4:128305/1‑137 (MQ=255)
                                                                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATgg     <  2:321569/135‑1 (MQ=255)
                                                                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa  <  7:169309/138‑1 (MQ=255)
                                                                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa  <  4:60538/138‑1 (MQ=255)
                                                                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa  <  4:65381/138‑1 (MQ=255)
                                                                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCAGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa  <  3:72123/138‑1 (MQ=255)
                                                                                                                                |                                                                                                                            
AAGTCTTGTTCATAACGATCAGCCAGTAGATTGTGCTTTTTGAATGTAATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAA  >  CP006881/2198399‑2198651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑