Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,572 C→T E137E (GAG→GAA)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,5720CT98.0% 150.6 / NA 50E137E (GAG→GAA) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/29);  minor base A (0/1);  total (20/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAA  >  CP006881/2198447‑2198700
                                                                                                                             |                                                                                                                                
atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                                                                                                                     <  2:151721/139‑1 (MQ=255)
atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCttttt                                                                                                                     <  6:254012/139‑1 (MQ=255)
atatTCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCtttt                                                                                                                      <  4:376529/138‑1 (MQ=255)
   ttCTTTTTGATTTCCGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTtcat                                                                                                                  <  6:89194/139‑1 (MQ=255)
                ccGTTTTCACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGc                                                                                                     >  4:331592/1‑139 (MQ=255)
                 cgttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGc                                                                                                     <  3:57383/135‑1 (MQ=255)
                  gttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGcta                                                                                                   <  5:25971/137‑3 (MQ=255)
                  gttttcACTTCATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGcta                                                                                                   <  7:103588/137‑3 (MQ=255)
                            cATAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGa                                                                                           <  3:331592/137‑1 (MQ=255)
                             aTAACTTCTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg                                                                                        <  7:254042/139‑1 (MQ=255)
                             aTAACTTCTATTTGCGTTATGCGTAATATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAATACCGCTCCTGATAATCGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAg                                                                                        <  2:365270/139‑1 (MQ=255)
                                    cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata                                                                                         <  4:315547/131‑3 (MQ=255)
                                    cTATTTGCGTTTTGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGata                                                                                         >  3:315547/1‑129 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                 <  5:380305/136‑1 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                 <  5:68061/136‑1 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                 <  6:19195/136‑1 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                 <  3:296291/136‑1 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                 <  1:347301/136‑1 (MQ=255)
                                       tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAc                                                                              >  2:51100/1‑139 (MQ=255)
                                          gcgttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAcct                                                                            <  4:98203/135‑3 (MQ=255)
                                            gttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACctttt                                                                         >  7:140764/2‑139 (MQ=255)
                                                ttgcgaaaaTAACGATCATTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTATCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGc                                                                      <  4:276149/133‑1 (MQ=255)
                                                 gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                                                                     <  7:4063/136‑1 (MQ=255)
                                                 gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                                                                     <  8:140764/136‑1 (MQ=255)
                                                            cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa                                                         >  7:123313/1‑139 (MQ=255)
                                                            cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa                                                         >  2:249941/1‑139 (MQ=255)
                                                              aTCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATg                                                       >  5:150741/1‑139 (MQ=255)
                                                                 tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGATGACCTTTTTGCTGGTCCAAAAGCATGGc                                                     >  6:413373/2‑138 (MQ=255)
                                                                 tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc                                                     >  4:438552/2‑138 (MQ=255)
                                                                  ttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc                                                     >  4:128305/1‑137 (MQ=255)
                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATgg                                                      <  2:321569/135‑1 (MQ=255)
                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                   <  4:65381/138‑1 (MQ=255)
                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                   <  4:60538/138‑1 (MQ=255)
                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                   <  7:169309/138‑1 (MQ=255)
                                                                   tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCAGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                   <  3:72123/138‑1 (MQ=255)
                                                                                  cTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctc                                   <  7:372215/139‑1 (MQ=255)
                                                                                   ttCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctcc                                  >  5:181863/1‑138 (MQ=255)
                                                                                     ccTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTcccc                                >  5:389967/1‑139 (MQ=255)
                                                                                             gACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGc                        >  3:112376/1‑139 (MQ=255)
                                                                                              aCCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGACTCCTCCCCCGTTgcg                         >  2:184476/1‑135 (MQ=255)
                                                                                                cgctcCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAgg                     >  7:303128/3‑139 (MQ=255)
                                                                                                             cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa                                                    <  8:212700/95‑1 (MQ=255)
                                                                                                             cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa                                                    >  7:212700/1‑95 (MQ=255)
                                                                                                             cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat        >  6:355671/1‑139 (MQ=255)
                                                                                                             cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat        >  2:283264/1‑139 (MQ=255)
                                                                                                               ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAATCAAGGCAAGCGTGTATGGCTCCTCCCCCGTTGCGCAGGCGGCCCTCCaaatat      >  2:441274/1‑137 (MQ=255)
                                                                                                               ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaattt      >  6:223418/1‑137 (MQ=255)
                                                                                                                   ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa   <  6:389967/138‑1 (MQ=255)
                                                                                                                   ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa   <  6:150741/138‑1 (MQ=255)
                                                                                                                    tttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTaa  <  1:441274/138‑1 (MQ=255)
                                                                                                                             |                                                                                                                                
ATATTCTTTTTGATTTCCGCTTTTACTTCATAACTTCTATTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAA  >  CP006881/2198447‑2198700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑