Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,617 G→C P122P (CCC→CCG)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,6170GC97.7% 138.0 / NA 45P122P (CCC→CCG) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (20/23);  minor base A (0/1);  total (21/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAGAATAGCAGTCTCAAG  >  CP006881/2198486‑2198742
                                                                                                                                   |                                                                                                                             
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                                                           <  1:347301/136‑1 (MQ=255)
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                                                           <  5:380305/136‑1 (MQ=255)
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                                                           <  5:68061/136‑1 (MQ=255)
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                                                           <  3:296291/136‑1 (MQ=255)
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGaa                                                                                                                           <  6:19195/136‑1 (MQ=255)
tttgcgttttgcgtAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAc                                                                                                                        >  2:51100/1‑139 (MQ=255)
   gcgttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGAcct                                                                                                                      <  4:98203/135‑3 (MQ=255)
     gttttGCGTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACctttt                                                                                                                   >  7:140764/2‑139 (MQ=255)
         ttgcgaaaaTAACGATCATTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTATCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGc                                                                                                                <  4:276149/133‑1 (MQ=255)
          gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                                                                                                               <  7:4063/136‑1 (MQ=255)
          gcgTAAAATAACGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCt                                                                                                               <  8:140764/136‑1 (MQ=255)
                     cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa                                                                                                   >  7:123313/1‑139 (MQ=255)
                     cGATCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCa                                                                                                   >  2:249941/1‑139 (MQ=255)
                       aTCTTTCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATg                                                                                                 >  5:150741/1‑139 (MQ=255)
                          tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGATGACCTTTTTGCTGGTCCAAAAGCATGGc                                                                                               >  6:413373/2‑138 (MQ=255)
                          tttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc                                                                                               >  4:438552/2‑138 (MQ=255)
                           ttCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGc                                                                                               >  4:128305/1‑137 (MQ=255)
                            tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATgg                                                                                                <  2:321569/135‑1 (MQ=255)
                            tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                                                             <  7:169309/138‑1 (MQ=255)
                            tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                                                             <  4:65381/138‑1 (MQ=255)
                            tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                                                             <  4:60538/138‑1 (MQ=255)
                            tCACAGATAGCGGCACTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCAGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCaa                                                                                             <  3:72123/138‑1 (MQ=255)
                                           cTTCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctc                                                                             <  7:372215/139‑1 (MQ=255)
                                            ttCCTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctcctcc                                                                            >  5:181863/1‑138 (MQ=255)
                                              ccTGTAAAGACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTcccc                                                                          >  5:389967/1‑139 (MQ=255)
                                                      gACCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGc                                                                  >  3:112376/1‑139 (MQ=255)
                                                       aCCGCTCCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGACTCCTCCCCCGTTgcg                                                                   >  2:184476/1‑135 (MQ=255)
                                                         cgctcCTGATAAACGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAgg                                                               >  7:303128/3‑139 (MQ=255)
                                                                      cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa                                                                                              <  8:212700/95‑1 (MQ=255)
                                                                      cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCa                                                                                              >  7:212700/1‑95 (MQ=255)
                                                                      cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat                                                  >  6:355671/1‑139 (MQ=255)
                                                                      cGCCTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaat                                                  >  2:283264/1‑139 (MQ=255)
                                                                        ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAATCAAGGCAAGCGTGTATGGCTCCTCCCCCGTTGCGCAGGCGGCCCTCCaaatat                                                >  2:441274/1‑137 (MQ=255)
                                                                        ccTTTTTTCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCaaattt                                                >  6:223418/1‑137 (MQ=255)
                                                                            ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa                                             <  6:389967/138‑1 (MQ=255)
                                                                            ttttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTa                                             <  6:150741/138‑1 (MQ=255)
                                                                             tttCGCTTTTTCCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTaa                                            <  1:441274/138‑1 (MQ=255)
                                                                                    ttttcCAATGCTTTTTCATCAATATCCGTCGCTAATATCAGATAGCCCGGAAGACCTTTTTGCGGGTCCAATAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTag                                    <  8:314438/136‑2 (MQ=255)
                                                                                     tttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTag                                    <  8:428353/136‑2 (MQ=255)
                                                                                      ttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctc                                                                                >  2:428877/2‑93 (MQ=255)
                                                                                      ttcCAATGCTTTTTCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGctc                                                                                <  1:428877/92‑1 (MQ=255)
                                                                                                 tttCATCAATATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCAGGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTAGAGGTCTTGAtt                         <  4:112376/137‑1 (MQ=255)
                                                                                                        aaTATCCGTCGCTAAAATCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACGCCAAATTTTTTAAGGCCTAGAGGTCTTGATTAACGGCAg                 >  4:1523/1‑138 (MQ=255)
                                                                                                        aaTATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAg                 >  6:427556/1‑138 (MQ=255)
                                                                                                                      aaaTCTGATAGCCCGGAAGACCTTTTTGCTGGTCCAAAAGCATGGCAAGCGTGTATGGCTCCTCCCCCGTTGAGCAGGCGGCACTCCAAATTTTTAAAGGCCTAGAGGTCTTGATTAACGGCAGAATTGCAGTCTCAAg  <  6:282011/137‑1 (MQ=255)
                                                                                                                                   |                                                                                                                             
TTTGCATTTTGAGTAAAATAACGATCCTTCACAGATGAAGGTACTTCCTGTAAAGACCTCTCCTGATAGACGCCTTTTTTCGCTTTCTCCAATGCTTTTTCATCAATATCAGTCGCCAGGATCTGAAAGCCGGGAAGACTTTTTTGCTGATCCAAAAGCATGGCAAGCGTGTAAGGCTCCTCACCCGTTGAGCAGGCGGCACTCCATATCTTTAAAGGCTTACCGTTCTTGATTAACGGCAGAATAGCAGTCTCAAG  >  CP006881/2198486‑2198742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑