Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 2,198,863 A→G F40F (TTT→TTC)  U712_11015 ← Chemotaxis protein methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,198,8630AG97.7% 263.9 / NA 85F40F (TTT→TTC) U712_11015Chemotaxis protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (33/50);  minor base T (0/2);  total (33/52)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAATAGCAGTCTCAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGGGCTTGATCCTTTTCTAATGCCGCCGCAAAGTCCTTAAAGCTTTGAAACCCCTTTTTCTCATAAAGCGACGTCAGCCTTCTCTTCATTTGTGCTTCTTTATATAATGTCAAATCGACTCCGGTTAATTGTTTCCACTTCGTTGTAAATACGCTGTATGTATCCATCTCTCTACTCTCCCAGT  >  CP006881/2198727‑2198999
                                                                                                                                        |                                                                                                                                        
gAATTGCAGTCTCAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCATGAACGCTTTGgaa                                                                                                                                        >  4:255860/1‑139 (MQ=255)
       aGTCTCAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCttt                                                                                                                                 <  3:128305/139‑1 (MQ=255)
           tcAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTtct                                                                                                                             >  8:92376/1‑139 (MQ=255)
           tcAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTtc                                                                                                                              >  6:100566/1‑138 (MQ=255)
           tcAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTtc                                                                                                                              >  4:347115/1‑138 (MQ=255)
             aaGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCa                                                                                                                           >  6:208442/1‑139 (MQ=255)
              aGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCa                                                                                                                           >  4:114875/1‑138 (MQ=255)
                 aCTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATaaa                                                                                                                       <  3:267594/139‑1 (MQ=255)
                   ttCCCATCTTATATAATTACGATAAAATTCTGAAACATAGATCGTCATTCTGTCCAATGTAACATTTAAAAGAGATTGATCCTTATCCAATGCCGCAGCAAAGTCCTTAAAGCTTTCGAACCCCATTTTCTCTTAAAgt                                                                                                                     >  4:163253/1‑138 (MQ=255)
                     cccATCTTTTATAATTACTATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAgt                                                                                                                     >  5:18264/1‑136 (MQ=255)
                     cccATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGa                                                                                                                   >  3:198385/1‑139 (MQ=255)
                     cccATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGa                                                                                                                   >  6:361734/1‑139 (MQ=255)
                     cccATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGa                                                                                                                   >  5:253794/1‑139 (MQ=255)
                     cccATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCAAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGa                                                                                                                   >  7:216992/1‑139 (MQ=255)
                             ttATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGc                                                                                                            <  6:181863/138‑1 (MQ=255)
                               ataATTACGATAAAATTCTGAAACATTGATCGTCATTATGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATCCCGCAGCAAACTCCTAAAAGCTTAGGAACCCCTTTTCCTCATAAAGTGACGTTAGCCt                                                                                                          >  3:325497/1‑138 (MQ=255)
                                taATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCtt                                                                                                         <  3:177009/138‑1 (MQ=255)
                                  aTTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTtctc                                                                                                      <  5:86061/139‑1 (MQ=255)
                                    tACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTGctct                                                                                                     >  3:84211/1‑138 (MQ=255)
                                     aCGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCtt                                                                                                    >  5:252404/1‑138 (MQ=255)
                                     aCGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCtt                                                                                                    >  8:268225/1‑138 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  5:413373/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  6:18264/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  6:252404/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  3:124526/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  4:111038/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  1:40132/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  8:303128/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  7:158340/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  1:121077/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  3:361333/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  3:361172/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  1:283264/139‑1 (MQ=255)
                                            aaTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTtgtg                                                                                            <  5:183947/139‑1 (MQ=255)
                                             aTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGc                                                                                           <  3:255860/139‑1 (MQ=255)
                                             aTTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGc                                                                                           <  7:268225/139‑1 (MQ=255)
                                              ttCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGct                                                                                          <  3:268357/139‑1 (MQ=255)
                                                   aaaCATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGcttct                                                                                       <  2:62255/137‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  8:220933/139‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  3:85657/139‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  7:243793/139‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  3:206269/139‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  5:361734/139‑1 (MQ=255)
                                                      cATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  3:347115/139‑1 (MQ=255)
                                                      agacagtCGTCATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  3:291044/133‑1 (MQ=255)
                                                                cATTCTGTCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTACTTtata                                                                                  >  7:230997/1‑129 (MQ=255)
                                                                cATTCTGTCAAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  8:230997/129‑1 (MQ=255)
                                                                       tCCAATGTTTCATTTAAGAGATCTTGATCCTTTTCCAATTACGCAGCAAAGTCCTTAAAGCTTTGGAACCCCGTTTTCTAATAAATTGACGTTATACTTCTCTTCATTTGTGCTTCTTTAAATAGTGTTAAATCGACTc                                                                 >  8:334208/1‑139 (MQ=255)
                                                                       tCCAATGTTTCATTTAAGAGAGCTTGATCTTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAATGTGACGTTTGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACt                                                                  <  5:100566/138‑1 (MQ=255)
                                                                       tCCAATGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTGGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACt                                                                  <  7:137147/138‑1 (MQ=255)
                                                                            tGTTTCATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAATTCGACTCCGGt                                                             >  1:31665/1‑138 (MQ=255)
                                                                                 cATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  >  1:84693/1‑112 (MQ=255)
                                                                                 cATTTAAGAGAGCTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTtata                                                                                  <  2:84693/112‑1 (MQ=255)
                                                                                     aaaGAGAGCTTCATCCTTTTCCAATGCCCCCGCAAAGCATTTAAAGCTTTGGACCCCCTTTTGATCATAATGGCAGGTTCGCGTGCTCTTATTTTGGGCTTCTTTATATGGTGTTAAATCGACTCCGGTTAATTGttt                                                    <  4:230292/137‑1 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGGTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           >  3:40109/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           >  8:356225/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           >  1:99688/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           >  7:54449/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           >  4:13094/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATGTCGt                                           >  5:89655/1‑139 (MQ=255)
                                                                                             cTTGATCCTTTTCCAATGCCGCAGCAAAGTCATTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTCACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGGGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGt                                           <  1:184476/139‑1 (MQ=255)
                                                                                                gATCCTTTTCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTg                                         >  3:386215/1‑138 (MQ=255)
                                                                                                gATCCTTTACCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCATTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTAAAAAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTg                                         <  1:130246/138‑1 (MQ=255)
                                                                                                     ttttCCAATGCCGCAGCAAAGTCCTTAAAGCATTCGAACCACTTTTTCTCATAAAGAGACGTTACCCTTATCTTCATTAGTGCTTCTTTTATTAGTGTTAAATCGACTCCGGTTAAAACTTTCCAATTCGTAGTaaaa                                    >  7:142967/1‑137 (MQ=255)
                                                                                                      tttCCAATGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTATTTCTCATAAAGTGACGTTAGCCTACACTTCATTTGTGCTTCTTTATATAGTGATAAAAAGACTCCGGTTAATAGTTTCCATTTCGATGTAAATAc                                  >  5:136668/1‑139 (MQ=255)
                                                                                                        tccaaTGCCGCAGCAATGTCATTAACGCTTTGTAACCCCTTTTTGTCATAATGTGAGGTTCGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACg                                 <  8:54449/135‑1 (MQ=255)
                                                                                                        tccaaTGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACTTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACg                                 <  3:114875/135‑1 (MQ=255)
                                                                                                        tccaaTGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATCGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACg                                 <  5:188295/135‑1 (MQ=255)
                                                                                                        tccaaTGCCGCAGCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACg                                 <  4:435642/135‑1 (MQ=255)
                                                                                                         gaattGCGGCAGCAATGTCGTTAATGCTTTGGATCCCCTTTTTTTCATAATGTGACGTTTGCGTTCTCTTCATTTGTGCTTCTTTATATTGTGTTAATTCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGc                                <  6:136668/134‑1 (MQ=255)
                                                                                                         cctaTGCCGCAGCAATGTCCTTAAAGCTTTTGAACCGCTTTTCCAGATAATTTTAAGTTAGCGTTGTCTTTATTTGTGGTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATTCGc                                <  4:325497/135‑1 (MQ=255)
                                                                                                             tGCCGCAGCAACGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCtgtat                           <  6:412512/139‑1 (MQ=255)
                                                                                                              gccgaagcAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCtgtat                           <  8:216992/132‑1 (MQ=255)
                                                                                                               ccgcagCAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAATTACGCtgtatg                          >  8:413744/1‑138 (MQ=255)
                                                                                                               ccgcaccAAAGTCCTTAAAGTTTTGGAACCCCTTTTTCTAATAAAGTGAGGTTAGCCTTCTCTTCTTTTGTGATTCTTTATATAGTTTAAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCtgtatg                          <  3:39347/132‑1 (MQ=255)
                                                                                                                 gcagcAAAGTCCTTAAATCTTTGTAAGCCGTTTATCTCATAAAGTGACGTAAGCCGTCTATTCATTGGTGCTTCTTTATATAGTTTTAACTCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTgtatgtat                       <  8:321512/136‑1 (MQ=255)
                                                                                                                 gcagcAAAGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAATTACGCTgtatgtat                       >  4:79918/4‑139 (MQ=255)
                                                                                                                  cagaaaaGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTAATTTGTGCTTATTTATATAGTTTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATc                      <  6:253794/135‑1 (MQ=255)
                                                                                                                     cAATGTCTTAAAAGTTTTGGAACCCTTTTTTCTAATAATGTTCGTTTAGCGTTCTATTCTTTTTTGCTTATTTATTTTGTGTAAACTCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCAt                   <  4:40109/139‑1 (MQ=255)
                                                                                                                     aaatttCCTTAAAGGTTTGGAACCCCTTTTTCTCATAACGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCAt                   <  4:198385/134‑1 (MQ=255)
                                                                                                                      aaaGTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCAtc                  <  5:208442/139‑1 (MQ=255)
                                                                                                                         gTCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCAtctc                >  3:419173/1‑138 (MQ=255)
                                                                                                                          tCCTTAAAGCTTTGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCAtctcta              <  3:13094/139‑2 (MQ=255)
                                                                                                                                    tttGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCatt                                               <  7:51144/96‑3 (MQ=255)
                                                                                                                                    tttGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCatt                                               >  8:51144/1‑94 (MQ=255)
                                                                                                                                    tttGGAACCCCTTTTTCTCATAAAGTGACGTTAGCCTTCTCTTCATTTGTGCTTCTTTATATAGTGTTAAATCGACTCCGGTTAATTGTTTCCATTTCGTTGTAAATACGCTGTATGTATCCA‑‑TCTCTACTCTCCCAGt  >  5:98219/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GAATAGCAGTCTCAAGAACTTCCCATCTTTTATAATTACGATAAAATTCTGAAACATTGATCGTCATTCTGTCCAATGTTTCATTTAAGAGGGCTTGATCCTTTTCTAATGCCGCCGCAAAGTCCTTAAAGCTTTGAAACCCCTTTTTCTCATAAAGCGACGTCAGCCTTCTCTTCATTTGTGCTTCTTTATATAATGTCAAATCGACTCCGGTTAATTGTTTCCACTTCGTTGTAAATACGCTGTATGTATCCATCTCTCTACTCTCCCAGT  >  CP006881/2198727‑2198999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑