Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,141	T→A	D262V (GAT→GTT)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,141	0	T	A	100.0%	61.3 / NA	21	D262V (GAT→GTT)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/9);  total (12/9)

TGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCC  >  CP006881/2174008‑2174272                                                                                                                                      |                                                                                                                                    tgcttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtg                                                                                                                                >  4:305824/4‑138 (MQ=255)   cttttttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc                                                                                                                              <  3:305824/138‑4 (MQ=255)   cgattttCCCAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTAtgcc                                                                                                                              <  8:197962/136‑4 (MQ=255)        ttcccAGCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATAGTCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAgg                                                                                                                          <  4:179756/136‑2 (MQ=255)             agCTCTGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAAt                                                                                                                     >  3:386818/2‑138 (MQ=255)                  tGTACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                                                                                                  >  4:439254/1‑136 (MQ=255)                    tACATTGTCATTGCGTCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAgag                                                                                                             <  6:350233/139‑3 (MQ=255)                              ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAg                                                                                                   >  7:166910/5‑139 (MQ=255)                              ttgcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTAAAAAATATTACAAGGTTAATTAAAAGAGCAGCCCaaac                                                                                                   >  2:359990/5‑138 (MQ=255)                                gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                                                                                                  <  2:104026/120‑1 (MQ=255)                                gcgtCAAGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGttaattaa                                                                                                                  >  1:104026/3‑122 (MQ=255)                                     aaGGCTCTGGCCAAACGTATCGGCAATGTGAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTGTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCa                                                                                            >  5:100129/1‑139 (MQ=255)                                                               tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag                                                                          <  2:116458/131‑4 (MQ=255)                                                               tgtgAATGGCTTCCTTGATCACTTTTCCCGGGAGCTTCCAGGCTTGCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAagagaag                                                                          >  1:116458/1‑128 (MQ=255)                                                                          tccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTTAAAGATCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgtgg                                                       >  3:241331/1‑136 (MQ=255)                                                                           ccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgttg                                                       >  4:298788/1‑135 (MQ=255)                                                                                               aGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTAt                                  >  1:66109/1‑139 (MQ=255)                                                                                                            tCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAg                      >  8:34147/1‑138 (MQ=255)                                                                                                               ggAAAAGGTGCGCGTCTTTCAAAACTATGCAATGGTTAATTTAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGCTTTCTCGTTTTTGATAAAAACCAggaat                  <  6:393025/139‑4 (MQ=255)                                                                                                                   aaGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCTAAGCTCTTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct              <  4:34442/139‑2 (MQ=255)                                                                                                                                 tCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatctc  <  6:407125/137‑6 (MQ=255)                                                                                                                                      |                                                                                                                                    TGATTTTTCCCCGGCTTTGTACATTGACATAGTGTCAAGGCTCTGGCCAAACGTATGGGAAATGTGAATCGCTTCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCC  >  CP006881/2174008‑2174272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑