Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	821,854	A→G	F181S (TTC→TCC)	U712_03970 ←	Trifunctional nucleotide phosphoesterase protein YfkN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	821,854	0	A	G	100.0%	167.9 / NA	52	F181S (TTC→TCC)	U712_03970	Trifunctional nucleotide phosphoesterase protein YfkN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/26);  total (26/26)

TTCGTTTTGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGAATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACTTTCTGCTCATTACCG  >  CP006881/821719‑821967                                                                                                                                        |                                                                                                                  ttCGTTTTGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                >  5:320968/1‑139 (MQ=255)  tCGTTTTGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                <  6:320968/138‑1 (MQ=255)  tCGTTTTGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                <  3:177138/138‑1 (MQ=255)  tCGGTTTGGGGGCAAGGTCAAACACGGCGTTGTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                <  6:334507/138‑1 (MQ=255)       ttGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACg                                                                                                           <  1:62182/138‑1 (MQ=255)        tggtggCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGa                                                                                                          >  8:127670/1‑138 (MQ=255)                  gTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCAcc                                                                                               <  8:21697/139‑1 (MQ=255)                            gCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGcc                                                                                     >  6:94881/1‑139 (MQ=255)                             cGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGccc                                                                                    >  1:379873/1‑139 (MQ=255)                             cGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGccc                                                                                    >  6:42150/1‑139 (MQ=255)                             cGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGccc                                                                                    >  7:286951/1‑139 (MQ=255)                             cGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGccc                                                                                    >  2:65051/1‑139 (MQ=255)                             cGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGccc                                                                                    >  3:270219/1‑139 (MQ=255)                                 ttCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTc                                                                                 >  6:160686/1‑138 (MQ=255)                                  tCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGa                                                                                                          <  3:171015/112‑1 (MQ=255)                                  tCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGa                                                                                                          >  4:171015/1‑112 (MQ=255)                                      cgcCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGAt                                                                            <  5:160686/138‑1 (MQ=255)                                      cgcCTGATGATTGCGCCTGCTTTTCAATGCAGGTGTGGGCGAGGTCAATAATGACATCTGCGCCTTCCGACTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGAt                                                                            <  8:426399/138‑1 (MQ=255)                                            atgatTGCGCCTGCTTTTCAATGCCGGTGTGGGCGCGGGCAATAATGACATCTGCGCCTTCCGCCTTCTGTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGACCTTGCCCCTCGAGATTCttttt                                                                     >  6:132008/1‑139 (MQ=255)                                            atgatTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCttttt                                                                     >  4:51898/1‑139 (MQ=255)                                            atgatTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCttttt                                                                     >  2:54689/1‑139 (MQ=255)                                            atgatTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCttt                                                                       <  7:127670/137‑1 (MQ=255)                                               atTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTc                                                                  <  4:270219/139‑1 (MQ=255)                                               atTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTc                                                                  <  1:54689/139‑1 (MQ=255)                                                  gcgcCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTccc                                                                <  5:42150/138‑1 (MQ=255)                                                   cgcCTGCTTTTCCATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCAt                                                              <  2:114353/139‑1 (MQ=255)                                                                 tGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTgcgg                                                <  5:200251/139‑1 (MQ=255)                                                                 tGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTgcgg                                                >  6:211522/1‑139 (MQ=255)                                                                  gCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTgcggc                                               <  2:220702/139‑1 (MQ=255)                                                                   ccGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGAt                                                                            >  7:310660/1‑109 (MQ=255)                                                                   ccGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGAt                                                                            <  8:310660/109‑1 (MQ=255)                                                                   ccGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTgcggc                                               <  5:226233/138‑1 (MQ=255)                                                                       tttgGGCGATGGCAATAATGACATCTGCGCCTTCCTCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGAGGTAc                                          <  8:286951/137‑1 (MQ=255)                                                                       tgtgGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGAc                                          <  1:413070/139‑1 (MQ=255)                                                                       tgtgGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGAc                                          <  5:168442/139‑1 (MQ=255)                                                                       gtgtggCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGAc                                          <  1:153258/135‑1 (MQ=255)                                                                                gggCAATAATGACATCCGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTAt                                 >  4:107689/1‑139 (MQ=255)                                                                                 ggCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGAtt                                                     <  4:147293/118‑1 (MQ=255)                                                                                 ggCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGAtt                                                     >  3:147293/1‑118 (MQ=255)                                                                                    aataatGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAg                             <  5:211522/139‑1 (MQ=255)                                                                                      taatGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGcc                           >  1:101639/1‑139 (MQ=255)                                                                                           aCATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                <  6:407814/49‑1 (MQ=255)                                                                                           aCATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGAtt                                                                                                                >  5:407814/1‑49 (MQ=255)                                                                                           aCATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACtt                      >  7:325385/1‑139 (MQ=255)                                                                                             aTCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAACGCCTATGTAGCCGACttt                     >  2:19630/1‑138 (MQ=255)                                                                                                 gcgcCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTTCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGTCAAAGCCTATGTAGCCGACTTTTACtt                >  6:159981/1‑139 (MQ=255)                                                                                                  cgccttccgccttcATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACttt               >  4:1548/1‑139 (MQ=255)                                                                                                  cgccttccgccttcATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACttt               >  7:36293/1‑139 (MQ=255)                                                                                                  cgccttccgccttcATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTCTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACttt               <  3:320832/139‑1 (MQ=255)                                                                                                     cttccgccttcATTTTCGGGATAGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACTTTCTg            <  7:105774/139‑1 (MQ=255)                                                                                                       tccgccttcATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTACCATGTCATGATTTGCGGCGGGACAAAGCATATGTAGCCGACTTTTACTTTCtcgt          >  5:95486/1‑136 (MQ=255)                                                                                                               cATTTTCGGGATCGTTTCATTGGCGGATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACTTTCTGCTCATTACCg  >  7:167232/1‑139 (MQ=255)                                                                                                                                        |                                                                                                                  TTCGTTTTGGTGGCAAGGTCAAACACGGCGTTTTCTGCGCCTGATGATTGCGCCTGCTTTTCAATGCCGGTGTGGGCGAGGGCAATAATGACATCTGCGCCTTCCGCCTTCATTTTCGGGATCGTTTCATTGGCGAATTCTACGATATCCTGCACCTGAACTTGCCCCTCGAGATTCTTTTTGTCCCATGTCATGATTTGCGGCGGGACAAAGCCTATGTAGCCGACTTTTACTTTCTGCTCATTACCG  >  CP006881/821719‑821967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑