Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,198	T→A	Y243F (TAC→TTC)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,198	0	T	A	100.0%	80.2 / NA	27	Y243F (TAC→TTC)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/14);  total (13/14)

TCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAA  >  CP006881/2174081‑2174331                                                                                                                      |                                                                                                                                      tccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTTAAAGATCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgtgg                                                                                                                  >  3:241331/1‑136 (MQ=255)  ccttgATCACTTTTCCCGGGAGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTcgttg                                                                                                                  >  4:298788/1‑135 (MQ=255)                      aGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTAt                                                                                             >  1:66109/1‑139 (MQ=255)                                   tCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAg                                                                                 >  8:34147/1‑138 (MQ=255)                                      ggAAAAGGTGCGCGTCTTTCAAAACTATGCAATGGTTAATTTAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGCTTTCTCGTTTTTGATAAAAACCAggaat                                                                             <  6:393025/139‑4 (MQ=255)                                          aaGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCTAAGCTCTTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                         <  4:34442/139‑2 (MQ=255)                                                        tCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatctc                                                             <  6:407125/137‑6 (MQ=255)                                                                 tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                               <  2:248901/123‑4 (MQ=255)                                                                 tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                               >  1:248901/4‑123 (MQ=255)                                                                 tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATg                                                    <  6:409819/134‑1 (MQ=255)                                                                 tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                  >  5:153100/4‑139 (MQ=255)                                                                 tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                  >  8:46152/4‑139 (MQ=255)                                                                           aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                         <  8:356785/106‑2 (MQ=255)                                                                           aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                         >  7:356785/1‑105 (MQ=255)                                                                           aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAg                                         <  4:94325/138‑1 (MQ=255)                                                                                 aaGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTgg                                  >  7:102138/1‑139 (MQ=255)                                                                                   gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                <  7:11422/137‑1 (MQ=255)                                                                                   gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                >  5:38443/3‑139 (MQ=255)                                                                                   gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                <  6:100129/137‑1 (MQ=255)                                                                                     gcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCgag                              <  7:19512/139‑3 (MQ=255)                                                                                                   ctCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGTTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTATGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTg                 >  6:275786/2‑138 (MQ=255)                                                                                                         ggATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTgcagcaat          <  4:241331/139‑3 (MQ=255)                                                                                                             gTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTtc       >  5:401310/1‑138 (MQ=255)                                                                                                                aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa    >  7:232617/1‑138 (MQ=255)                                                                                                                aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa    <  6:318709/138‑1 (MQ=255)                                                                                                                aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa    <  8:88562/138‑1 (MQ=255)                                                                                                                 aaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCaaa  <  5:114376/139‑1 (MQ=255)                                                                                                                      |                                                                                                                                      TCCTTCATCACTTTTCCCGGAAGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAA  >  CP006881/2174081‑2174331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑