Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,200,126	T→C	Q208Q (CAA→CAG)	U712_11025 ←	Heptaprenyl diphosphate synthase component 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,200,126	0	T	C	100.0%	184.5 / NA	57	Q208Q (CAA→CAG)	U712_11025	Heptaprenyl diphosphate synthase component 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/36);  total (21/36)

AGCTGATTCTTTAATGCTGGATTTTCCAGAGCATACAGCACTGGCAATGTGACATTCCCTTGAAGCAAATCTCCTCCAACGGGTTTGCCCAGCTCTTCTTCAGTTGAAGTAAAATCAAGGATATCATCAATGATTTGATAAGACATGCCGACGTAATACCCAAACCAGTACAGCGCCTTATGAATCTTTTCATCTGCTCCGGACGCAATGGCACCGAGCTGACAGCTGACCGCGATCAGGAGCGCCGTTTTTCTTTTGATGCG  >  CP006881/2199992‑2200254                                                                                                                                       |                                                                                                                                 aGCTGGTTTTTTAATGCAGGATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGa                                                                                                                               <  3:320700/138‑1 (MQ=255)     ggttttttAATGCAGGATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGa                                                                                                                          >  1:366461/6‑139 (MQ=255)      gttttttAATGCAGGATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGa                                                                                                                          >  4:28955/5‑138 (MQ=255)       ttttttAATGCAGGATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACa                                                                                                                        >  7:111208/4‑139 (MQ=255)             aaTGCAGGATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCg                                                                                                                   <  1:11344/138‑1 (MQ=255)                 caggATTTTTCAGGGCCTACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTa                                                                                                              <  3:90868/137‑1 (MQ=255)                 caggATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTa                                                                                                              <  5:89392/137‑1 (MQ=255)                 caggATTTTTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTa                                                                                                              <  5:423592/137‑1 (MQ=255)                      ttttTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  5:38595/139‑1 (MQ=255)                      ttttTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  6:433446/139‑1 (MQ=255)                      ttttTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  7:98708/139‑1 (MQ=255)                      ttttTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  1:187836/139‑1 (MQ=255)                      ttttTCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  8:137590/139‑1 (MQ=255)                       ttttCAGGGCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  2:326246/138‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  6:439299/135‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  2:80748/135‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  2:99743/135‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  3:219085/135‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  5:209922/135‑1 (MQ=255)                            agggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTGGAAGTAAAATCACGACTATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAAc                                                                                                    <  3:214727/135‑1 (MQ=255)                             gggCATACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCa                                                                                                  <  8:347879/137‑1 (MQ=255)                                  tACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACa                                                                                             <  8:26388/139‑1 (MQ=255)                                  tACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACa                                                                                             <  4:170168/139‑1 (MQ=255)                                  tACAGCACTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACa                                                                                             <  2:349751/139‑1 (MQ=255)                                        aCTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGAt                                                                                                                              <  6:196198/100‑1 (MQ=255)                                        aCTGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGAt                                                                                                                              >  5:196198/1‑100 (MQ=255)                                          tGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCg                                                                                                                   <  6:260605/109‑1 (MQ=255)                                          tGGCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCg                                                                                                                   >  5:260605/1‑109 (MQ=255)                                            gCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         >  2:390955/1‑117 (MQ=255)                                            gCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATAcc                                                                                                         <  1:390955/117‑1 (MQ=255)                                            gCAATGTGACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTAt                                                                                    >  2:359150/1‑138 (MQ=255)                                                 gtgACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCa                                                                                                  <  2:47660/119‑1 (MQ=255)                                                 gtgACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCa                                                                                                  >  1:47660/1‑119 (MQ=255)                                                 gtgACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCt                                                                              >  4:212631/1‑139 (MQ=255)                                                  tgACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCtt                                                                             <  8:254491/139‑1 (MQ=255)                                                  tgACGTTTCCTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCtt                                                                             <  6:440489/139‑1 (MQ=255)                                                          ccTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATcag                                                                     >  5:396037/1‑137 (MQ=255)                                                           cTTGAAGCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATcag                                                                     <  6:396037/138‑3 (MQ=255)                                                                 gCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTTATACTAAAATCAGTACAATTCCTTATTAATCTTCTCATCAGCTCCAGa                                                              <  8:111208/139‑1 (MQ=255)                                                                 gCAAATCTCCTCCTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGa                                                              <  6:290352/139‑1 (MQ=255)                                                                          ctccTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCCAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGCCGCAATGGc                                                     >  2:210909/1‑139 (MQ=255)                                                                          ctccTACGGGTTTACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCaa                                                         <  1:353502/135‑1 (MQ=255)                                                                                    tttACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAGTAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAagctgg                                           >  5:16414/1‑138 (MQ=255)                                                                                    tttACCCAGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAAACTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAagctgg                                           >  7:265492/1‑138 (MQ=255)                                                                                           aGCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAagctggcagctga                                    <  6:394626/139‑1 (MQ=255)                                                                                            gCTCTTCCTCAGTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCATATCAATCTTCTCCTCAGCTCCAGACGCAATGGCACCAagctggcagctgac                                   <  7:76730/139‑1 (MQ=255)                                                                                             ctctTCCTCAGTTGAAGTAAAATCAAGAATATAATAAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGACTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGAcc                                  >  3:326235/1‑139 (MQ=255)                                                                                                       gTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGa                                                                                  >  1:129592/1‑81 (MQ=255)                                                                                                       gTTGAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGa                                                                                  <  2:129592/81‑1 (MQ=255)                                                                                                          gAAGTAAAATCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGATCCAGACGCAATGCCACCAAGCTGGCAGCTGACCGCGATCAAGatag                     >  2:399490/1‑136 (MQ=255)                                                                                                                 aaTCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCAAGAGAGCTGtttt              <  1:258775/139‑1 (MQ=255)                                                                                                                  aTCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCAAGAGAGCTGttttt             >  4:319284/1‑139 (MQ=255)                                                                                                                  aTCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCAAGAGAGCTGttttt             >  8:224867/1‑139 (MQ=255)                                                                                                                  aTCAAGAATATCATCAATAATCTGATAAGACATGCCGACGTAATACCCAAACAAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCAAGAGAGCTGtttt              >  3:393549/1‑138 (MQ=255)                                                                                                                            tcatcaATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCAAGAGAGCTGTTTTTCTTTTGAta    <  6:18904/138‑2 (MQ=255)                                                                                                                            tcatcaATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGATGGCAGCTGACCGCGATCAAGAGAGCTGTTTTTCTTTTGAtac   >  4:299296/1‑137 (MQ=255)                                                                                                                             catcaATAATCTGATAAGACATGCCGACGTAATACCCAAACCAGTACAATGCCTTATGAATCTTCTCATCAGCTCCAGACGCAATGGCACCAAGCTGGCAGCTGACCGCGATCATGAGAGCTGTTTTTGTTTTGATacg  >  1:346660/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                                 AGCTGATTCTTTAATGCTGGATTTTCCAGAGCATACAGCACTGGCAATGTGACATTCCCTTGAAGCAAATCTCCTCCAACGGGTTTGCCCAGCTCTTCTTCAGTTGAAGTAAAATCAAGGATATCATCAATGATTTGATAAGACATGCCGACGTAATACCCAAACCAGTACAGCGCCTTATGAATCTTTTCATCTGCTCCGGACGCAATGGCACCGAGCTGACAGCTGACCGCGATCAGGAGCGCCGTTTTTCTTTTGATGCG  >  CP006881/2199992‑2200254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑