Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,382,042	G→C	I77M (ATC→ATG)	U712_12100 ←	Uncharacterized protein YqgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,382,042	0	G	C	100.0%	155.2 / NA	50	I77M (ATC→ATG)	U712_12100	Uncharacterized protein YqgZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (22/28);  total (22/28)

ACCGTTTTCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTGATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTTCTCTGAATAAGTGCC  >  CP006881/2381908‑2382175                                                                                                                                       |                                                                                                                                      aCCGTTTTCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCAttt                                                                                                                                   <  1:107775/139‑1 (MQ=255) aCCGTTTTCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCAttt                                                                                                                                   <  3:87021/139‑1 (MQ=255)     ttttCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATttcttc                                                                                                                               <  4:221116/139‑1 (MQ=255)     ttttCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATttctt                                                                                                                                >  7:278202/1‑138 (MQ=255)     ttttCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTACTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATttctt                                                                                                                                >  3:157407/1‑138 (MQ=255)            cttcGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGtt                                                                                                                         <  2:113680/138‑1 (MQ=255)            cttcGATAACTTCAACAGGTCTCCAGTGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGtt                                                                                                                         <  1:30549/138‑1 (MQ=255)                           cAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTg                                                                                                         <  4:157407/139‑1 (MQ=255)                            aGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGa                                                                                                        <  7:228854/139‑1 (MQ=255)                              ttCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCAt                                                                                                                                     >  1:432394/1‑108 (MQ=255)                              ttCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCAt                                                                                                                                     <  2:432394/108‑1 (MQ=255)                                ctcCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAg                                                                                                     >  1:418709/1‑138 (MQ=255)                                          ataACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGcttctt                                                                                          >  3:185583/1‑139 (MQ=255)                                           taACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTg                                                                                         <  7:212662/139‑1 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGcc                                                                                    >  4:420365/1‑138 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGcc                                                                                    >  2:371925/1‑138 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGcc                                                                                    >  7:276390/1‑138 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGcc                                                                                    >  3:224400/1‑138 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTAACGTCATTTCTTCAATGTATAAATTTAAATTTTTTAAAGTCTGGCTTCTTGTCGcc                                                                                    >  3:297817/1‑138 (MQ=255)                                                 aTCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATATACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGATTCTTGTCGcc                                                                                    >  5:396126/1‑138 (MQ=255)                                                      cAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAAtt                                                                              >  2:130662/1‑139 (MQ=255)                                                            tttGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTtcatc                                                                         >  2:298676/1‑138 (MQ=255)                                                                   tCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCCCCTCATTTACCGTCATTTCTTCAATGTTTAATTTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTGATCAATTCCtt                                                                 >  1:373100/1‑139 (MQ=255)                                                                   tCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCtt                                                                 >  1:367342/1‑139 (MQ=255)                                                                   tCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCt                                                                  >  8:340639/1‑138 (MQ=255)                                                                           aaTCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTATAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCggt                                                         <  6:71327/139‑3 (MQ=255)                                                                            aTCGGGCGTCGCTAAAGCTTCGGCTTCTAGATTTAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCATTCTGTCGtt                                                         <  4:297817/138‑1 (MQ=255)                                                                            aTCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTa                                                        <  2:224983/139‑1 (MQ=255)                                                                             tCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTaa                                                       <  5:6713/139‑1 (MQ=255)                                                                             tCGCGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAACACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTaa                                                       <  6:377477/139‑1 (MQ=255)                                                                               gggCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACg                                                     <  1:122435/139‑1 (MQ=255)                                                                                ggCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTTCTTCTGTCGTTAACGa                                                    >  5:54796/1‑139 (MQ=255)                                                                                 gCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGa                                                    >  5:330202/1‑138 (MQ=255)                                                                                  cGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGAt                                                   >  5:33452/1‑138 (MQ=255)                                                                                  cGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGAt                                                   >  2:339440/1‑138 (MQ=255)                                                                                       cAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATg                                             <  4:224400/139‑1 (MQ=255)                                                                                       cAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATg                                             <  7:340639/139‑1 (MQ=255)                                                                                       cAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATg                                             <  3:420365/139‑1 (MQ=255)                                                                                       cAAAAGCTTCCGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATg                                             <  2:367342/139‑1 (MQ=255)                                                                                            gCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACtt                                        <  7:353746/139‑1 (MQ=255)                                                                                                      ctcGATTAAAGGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATtcttct                              >  1:122532/1‑139 (MQ=255)                                                                                                      ctcGATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAAttctt                                <  5:160703/137‑1 (MQ=255)                                                                                                         gATTAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTtctc                             <  5:99140/137‑1 (MQ=255)                                                                                                           ttAAAAGTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGt                         <  8:276390/139‑1 (MQ=255)                                                                                                                 gTTCCAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGt                   <  7:65367/139‑1 (MQ=255)                                                                                                                    ccAGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGttt                 <  1:130662/138‑1 (MQ=255)                                                                                                                      aGCACCTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTtctc              <  1:371925/139‑1 (MQ=255)                                                                                                                           cTCATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTTCTCTGAAt         <  2:418709/139‑1 (MQ=255)                                                                                                                             cATTTACCGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTTCTCTGAAt         >  5:88509/1‑137 (MQ=255)                                                                                                                                   acGTCATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTTCTCTGAATAAGTGcc  <  4:12059/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACCGTTTTCTTCTTCGATAACTTCAACAGTTCTCCCGGGTTATAACCAATCACCAGCTTTTTGTTGTCTACTAGAATCGGGCGTCGCAAAAGCTTCGGCTTCTCGATTAAAAGTTCCAGCACCTCATTTACCGTGATTTCTTCAATGTTTAAATTTAAATTTTTGAAAGTCTGGCTTCTTGTCGCCAAAATTTCATCAATTCCTTCTGTCGTTAACGATAAAATGTACTTTAATTCTTCTCTTGTCGGGGTTTCTCTGAATAAGTGCC  >  CP006881/2381908‑2382175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑