Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,800,291	C→T	intergenic (‑168/‑62)	U712_14425 ← / → U712_14430	Citrate synthase 2/Hypothetical Protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,800,291	0	C	T	98.8%	253.7 / NA	81	intergenic (‑168/‑62)	U712_14425/U712_14430	Citrate synthase 2/Hypothetical Protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (32/48);  minor base A (1/0);  total (33/48)
Fisher's exact test for biased strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGAGTGTTGTAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTATGAAAGAACATAACAAATTCTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGTTTTTGCA  >  CP006881/2800158‑2800425                                                                                                                                      |                                                                                                                                        aaGAGTGTTGTAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     <  5:306806/138‑1 (MQ=255) aaGAGTGTTGTAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     <  5:185313/138‑1 (MQ=255)   gagTGTTGTAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAg                                                                                                                                  <  5:53505/139‑1 (MQ=255)      tgttgtAAAAACATGAGGCTAACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCt                                                                                                                                >  2:231890/1‑138 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAACCCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAt                                                                                                                             >  3:239614/1‑139 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAg                                                                                                                                  <  6:35829/134‑1 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAg                                                                                                                                  >  5:35829/1‑134 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAt                                                                                                                             >  7:210519/1‑139 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAt                                                                                                                             >  8:171463/1‑139 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAt                                                                                                                             >  3:253375/1‑139 (MQ=255)        ttgtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAt                                                                                                                             >  3:296377/1‑139 (MQ=255)          gtAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtt                                                                                                                            <  2:279329/138‑1 (MQ=255)           tAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAACAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAttt                                                                                                                           <  5:372293/138‑1 (MQ=255)           tAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAttt                                                                                                                           <  8:391646/138‑1 (MQ=255)           tAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAttt                                                                                                                           <  5:299077/138‑1 (MQ=255)           tAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAttt                                                                                                                           <  6:66785/138‑1 (MQ=255)           tAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGAAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAttt                                                                                                                           <  3:278756/138‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  4:363028/139‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  4:18153/139‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  6:307326/139‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  8:210519/139‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  2:276167/139‑1 (MQ=255)               aaCATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtt                                                                                                                      <  5:96416/139‑1 (MQ=255)                  aTGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagtttaa                                                                                                                   <  4:379408/139‑1 (MQ=255)                  aTGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagttt                                                                                                                     >  8:214758/1‑137 (MQ=255)                     aGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGcc                                                                                                                >  7:53787/1‑139 (MQ=255)                          tacttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATtc                                                                                                           >  3:441980/1‑139 (MQ=255)                           acttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTCCTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATtct                                                                                                          >  8:167521/1‑139 (MQ=255)                           acttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGTCTGCTGGAACTAAAACCCTTTGATTTTGAAATAACATAACAAATTTTTATTAGATTATTTAAGTTTAAGCCTATtct                                                                                                          >  3:286287/1‑139 (MQ=255)                           acttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTAtt                                                                                                            <  4:441980/137‑1 (MQ=255)                           acttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATtc                                                                                                           <  2:421841/138‑1 (MQ=255)                            cttaAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATtct                                                                                                          >  6:204358/1‑138 (MQ=255)                                aaaaGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCAtt                                                                                                      <  8:53787/138‑1 (MQ=255)                                 aaaGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATtat                                                                                                    <  7:252747/139‑1 (MQ=255)                                 aaaGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATta                                                                                                     >  5:170478/1‑138 (MQ=255)                                    gCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATaaa                                                                                                 <  8:330629/139‑1 (MQ=255)                                     cACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagttt                                                                                                                     >  4:318327/1‑118 (MQ=255)                                     cACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTAtttaagttt                                                                                                                     <  3:318327/118‑1 (MQ=255)                                        gATGCAAGTAATTCTCTCCCCCACAAAGAAAATTCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATCTAAGTTTAAGCCTATTCTCATTATAAACaat                                                                                             <  6:125215/139‑1 (MQ=255)                                             aaGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCa                                                                                        <  5:119805/139‑1 (MQ=255)                                               gTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGa                                                                                      <  5:28243/139‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACTATAATCAGACAt                                                                                   <  5:134164/139‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACa                                                                                    <  5:140895/138‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACa                                                                                    <  2:30999/138‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACa                                                                                    <  2:255108/138‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACAt                                                                                   <  4:92110/139‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACAt                                                                                   <  8:344032/139‑1 (MQ=255)                                                  tttCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACAt                                                                                   >  8:7934/1‑139 (MQ=255)                                                    tctctcCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     >  6:251538/1‑87 (MQ=255)                                                    tctctcCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     <  5:251538/87‑1 (MQ=255)                                                          ccccAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGtt                                                                              <  3:342764/136‑1 (MQ=255)                                                          ccccAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGtt                                                                              <  6:70450/136‑1 (MQ=255)                                                          ccccAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGtt                                                                              <  8:378151/136‑1 (MQ=255)                                                          ccccAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTaaa                                                                           >  1:45225/1‑139 (MQ=255)                                                          ccccAAAAAGAAAATGCATACATTTTACTGGCTGCTGCAACATAATCCCTATGCTTTTGAAAGAACATAACATATTATTATTAGCTTATTTAAGATTAAGCCTAATCTCATTATAAACAATAATCAGACATTTGTTaaa                                                                           >  7:19369/1‑139 (MQ=255)                                                          acccAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTaaa                                                                           >  5:60520/2‑139 (MQ=255)                                                                      aaTGCATACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     >  3:206425/1‑69 (MQ=255)                                                                      aaTGCATACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTtat                                                                                                                                     <  4:206425/69‑1 (MQ=255)                                                                              cATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGtt                                                                              >  6:48037/1‑116 (MQ=255)                                                                              cATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGtt                                                                              <  5:48037/116‑1 (MQ=255)                                                                                  ttACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAAGATAACAAATTTTTATAAGCTTAATTAAGTTTAAGACTATTCTAATTATTATCAATAATCTGAGATTTGTTAAATGTTTACCTATAA‑tttttttttt                                                   <  8:108591/138‑1 (MQ=255)                                                                                   tACTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTACAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTc                                                  >  3:234123/1‑138 (MQ=255)                                                                                    aCTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTcc                                                 <  3:152389/138‑1 (MQ=255)                                                                                     cTGGCTGCTGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCa                                                <  7:171463/138‑1 (MQ=255)                                                                                        gctgctGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGt                                                                        >  2:234336/1‑112 (MQ=255)                                                                                        gctgctGGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGt                                                                        <  1:234336/112‑1 (MQ=255)                                                                                            ctgGAACTAAAACCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTGCAttgggtgc                                        >  8:391251/1‑133 (MQ=255)                                                                                                      aaCCCTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGa                               >  5:54044/1‑138 (MQ=255)                                                                                                         ccTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGaaaaa                           <  2:342288/139‑1 (MQ=255)                                                                                                          cTTTGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGaaaaaa                          >  5:46527/1‑139 (MQ=255)                                                                                                             tGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAACCGc                       >  5:143903/1‑139 (MQ=255)                                                                                                             tGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGc                       >  8:43402/1‑139 (MQ=255)                                                                                                             tGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGc                       >  8:441248/1‑139 (MQ=255)                                                                                                             tGCTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGc                       >  1:396037/1‑139 (MQ=255)                                                                                                               cTTTTGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCt                     <  7:214758/139‑1 (MQ=255)                                                                                                                   tGAAAGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTAt                 >  4:71397/1‑139 (MQ=255)                                                                                                                       aGAACATAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTTCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGc             <  3:116155/139‑1 (MQ=255)                                                                                                                             tAACAAATTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGttt       <  1:231890/139‑1 (MQ=255)                                                                                                                              aaCAAATTTTTATAAGCTTAATTAAGTTTAAGCCTATTCTCATTATAAAAAATAATAAGACATTTGTTAAATGTTTACCTATAATTTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGttt       <  4:239614/139‑1 (MQ=255)                                                                                                                                  aaTTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGTTTTTGCa  <  7:323251/139‑1 (MQ=255)                                                                                                                                   aTTTTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGTTTTTGCa  >  4:385059/1‑138 (MQ=255)                                                                                                                                      |                                                                                                                                        AAGAGTGTTGTAAAAACATGAGGCTTACTTAAAAAGCACGATGCAAGTATTTCTCTCCCCCAAAAAGAAAATGCTTACATTTTACTGGCTGCTGGAACTAAAACCCTTTGCTTATGAAAGAACATAACAAATTCTTATAAGCTTATTTAAGTTTAAGCCTATTCTCATTATAAACAATAATCAGACATTTGTTAAATGTTTACCTATAA‑TTTTTTTTTTCCATTCTGTGATTTCCCCGAAAAAACGCCTTTATTTGCGGGTTTTTGCA  >  CP006881/2800158‑2800425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑