Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,209,530	G→A	A299T (GCA→ACA)	U712_16520 →	Spore germination protein A1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,209,530	0	G	A	100.0%	112.4 / NA	38	A299T (GCA→ACA)	U712_16520	Spore germination protein A1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/19);  total (19/19)

GGTGCCGGTTTCTCTCGGCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAGCACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAGCTGCTAAGG  >  CP006881/3209397‑3209661                                                                                                                                      |                                                                                                                                    ggTGCCGGTTTCTCTCGGCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCGTTGCTTCTATCTTTATCACCTTGTTTTTGTGATCGATTTATATAACACtt                                                                                                                                >  5:224869/1‑139 (MQ=255)   tGCCGGGTTCTCTCGGCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGt                                                                                                                              <  5:334423/139‑1 (MQ=255)                 ggCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATc                                                                                                                   <  8:361376/136‑1 (MQ=255)                 ggCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCa                                                                                                                  <  3:273495/137‑1 (MQ=255)                             cAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCGTTGCGTGTATCGTTTTCACCGTGGTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCgc                                                                                                    >  1:231823/1‑138 (MQ=255)                                tCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTGTTCTTGTATCTTTATAACCATTTTTTTGTCAACGATTTATATAACAAAATTTTCCTTCCATCAGGGGCGGGTGGCGGCCg                                                                                                 >  1:433208/1‑139 (MQ=255)                                tCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCGTTGCTTCTATCTTTATAACCTTGTTTTTGTAATCGAGTTATATAAAACAATTTTACATACATAACGGGCTGCAGCCGACCg                                                                                                 >  4:226790/1‑139 (MQ=255)                                      gATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCg                                                                                           <  5:80491/139‑1 (MQ=255)                                          actactATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGGTTCTATCTTTATGCCCTTGTTTTTGTCATCGATTTATATTACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGCCCGCACGCGCCGt                                                                                       >  5:206698/1‑139 (MQ=255)                                                     cGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATGTTTATAACCTTTTTTTTGTCATCGATTTATATAAAAATTGTTTCCTTCCATCAGGGGCTGCTTCCGACCGCACTCGCCGTCCCGATTTCgg                                                                            >  3:321486/1‑139 (MQ=255)                                                      gCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCGATCTTTATCACCTTGTTTTTGTCATCGATTTATATAAAACATGTTTACTTCCATCAGGGGCTGCTGCCGACCCCACACGCCCTCACCATTTAggg                                                                           >  4:384900/1‑138 (MQ=255)                                                          gATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGaa                                                                        <  8:69778/138‑1 (MQ=255)                                                                 gCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGaaa                                                                <  2:119806/139‑1 (MQ=255)                                                                 agacagctTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGaaa                                                                <  3:260153/133‑1 (MQ=255)                                                                    tctcTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACATGTTTACTTCCATCAGGGGCTGCTGCCGACCGCACACGCCGTCACGATTTCGGCGAACAGGGAAAACg                                                             >  3:442864/1‑139 (MQ=255)                                                                          aTCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTttcc                                                                                                                          >  6:198445/1‑72 (MQ=255)                                                                          aTCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTttcc                                                                                                                          <  5:198445/72‑1 (MQ=255)                                                                           tCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCATTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGt                                                       <  3:30248/138‑1 (MQ=255)                                                                            ccGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGtt                                                      <  2:433208/138‑1 (MQ=255)                                                                                tCGCTGCGCTTTGCGTCTATCTTTTTAACCTTGTTTTTGTGATAGATTTATTTAAAACTTTTTTACTTACAGAAGGGGCGGGTGCCGCCCGCCCGCGCCGGCACGCTTTCTGCGCACAGGGAAAACGTGCCGTTCCCTc                                                 >  6:341457/1‑139 (MQ=255)                                                                                                   tCTTTATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCctgc                               <  7:57283/138‑1 (MQ=255)                                                                                                      ttATCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGAt                           >  7:112785/1‑139 (MQ=255)                                                                                                        aTCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATgg                         >  2:378945/1‑139 (MQ=255)                                                                                                        aTCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATg                          >  3:260230/1‑138 (MQ=255)                                                                                                        aTCACCTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATg                          <  5:81625/138‑1 (MQ=255)                                                                                                             cTTGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTAGTCGAACAGGGAAAACGTGACGTTACCTCCGATATTTGAAGCCCTGCTGATGGAAGt                     >  6:83486/1‑138 (MQ=255)                                                                                                               tGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCAc                  >  8:93887/1‑139 (MQ=255)                                                                                                               tGTTTTTGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCAc                  >  2:162345/1‑139 (MQ=255)                                                                                                                 tttttGTCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCa                >  2:48501/1‑139 (MQ=255)                                                                                                                       tCATCTATTTATATAACACTTGTTTCCTTCCATCCGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCTATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  5:83486/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  5:413519/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  2:70435/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  3:163258/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  4:321486/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCAATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  4:158219/138‑1 (MQ=255)                                                                                                                       tCATCGATTTATATAACACTTGTTTCCTTCCATCAGCGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAg           <  7:93887/138‑1 (MQ=255)                                                                                                                           cGATTTATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAgctgc       >  6:420005/1‑138 (MQ=255)                                                                                                                               ttATATAACACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAGCTGCTAAgg  >  8:399218/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                    GGTGCCGGTTTCTCTCGGCATCCTGATGCAATCCCCGGATGACTACTATGAACGCTGGATTTCAGCATCTCTTATCCGGTCGCTGCGCTTTGCTTCTATCTTTATCACCTTGTTTTTGTCATCGATTTATATAGCACTTGTTTCCTTCCATCAGGGGCTGCTGCCGACCGCACTCGCCGTCACGATTTCGGCGAACAGGGAAAACGTGCCGTTCCCTCCGATATTTGAAGCCCTGCTGATGGAAGTCACCATCGAGCTGCTAAGG  >  CP006881/3209397‑3209661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑