| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,715,114 | A→G | M324V (ATG→GTG) | U712_19145 → | putative aldehyde dehydrogenase ywdH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,715,114 | 0 | A | G | 96.9% | 192.8 / ‑3.3 | 64 | M324V (ATG→GTG) | U712_19145 | putative aldehyde dehydrogenase ywdH |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (20/42); total (21/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACAGTACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAATGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCGCCCTGTATCTCTTCACCA > CP006881/3714984‑3715249 | acaGTACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCAGCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGAt < 1:139404/139‑1 (MQ=255) aGTACTGCAAAATCGTCAGGTAGCGCCACTATCAGCGGCTGCTCAGCTTTCTTAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGa < 8:349704/139‑1 (MQ=255) aGTACGTCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGa < 6:394980/139‑1 (MQ=255) aGTACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGa < 8:174050/139‑1 (MQ=255) aGTACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGa < 4:57562/139‑1 (MQ=255) tACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGAct < 4:211962/139‑1 (MQ=255) ggCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTgagaga > 4:167965/1‑131 (MQ=255) ggCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTgagaga < 3:167965/131‑1 (MQ=255) ggCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGActc > 3:339270/1‑137 (MQ=255) ggCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGActc < 4:339270/137‑1 (MQ=255) ggCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACtctc > 1:124895/1‑139 (MQ=255) cAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACtctc > 3:14244/1‑137 (MQ=255) cAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACtctc > 3:221383/1‑137 (MQ=255) aaaaTGGTCAGGGAGCGCAACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAg < 2:124895/139‑1 (MQ=255) aaTCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAATGAGAGATGACTCTCCAGt > 6:340616/1‑138 (MQ=255) tCGTCAGTGAGCGCCAGTATCAGCGGCTGCTCAGCATTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCGCGAACAAGTGATAGATGACTCTCCAGTGAt > 8:156649/1‑139 (MQ=255) tCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGAt > 2:239756/1‑139 (MQ=255) cgcCACTTTCCGCGGGTGCTCAGTTTTCTGAATGATGGAATCCCGGTCTCAGGCGGACAATGTGTTCCCAATCTTCACAAAATTGCGCCGACCTTTCTCGAACAAGTGTGAGATGACTCTCCAGTGATGCAGGAGgaga < 6:370499/139‑1 (MQ=255) tCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCggg > 8:6108/1‑139 (MQ=255) tgctCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttct > 7:134591/1‑139 (MQ=255) gctCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAATGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttctt < 5:340616/139‑1 (MQ=255) ctCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttcttc > 5:28509/1‑139 (MQ=255) ctCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTCATGCAGGAGGAGATTTTCGGGCCGAttcttc > 5:353450/1‑139 (MQ=255) ctCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATACTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttcttc < 6:401989/139‑1 (MQ=255) cAGCTTTCTGAATGATGGCATCCCGCGCACCGGCGGACAATCTGACCCCAATCATCACAAAATTGCGCCGACCATTCTCGACCACGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGA‑TTTCGGGCCGATTCTTCCGc < 3:50846/139‑1 (MQ=255) aGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTcc < 4:14244/137‑1 (MQ=255) aGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTcc < 7:112108/137‑1 (MQ=255) aGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTcc < 6:168557/137‑1 (MQ=255) aGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTcc < 7:6108/137‑1 (MQ=255) aGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCAAAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTcc < 8:187483/137‑1 (MQ=255) aGCTTGCTGAATGATGGCATCCCGCTCCCAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGCGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGc < 8:372448/139‑1 (MQ=255) aGCTTGCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAAACATCACAAAATTGCGCCGACCATTCTCGAACAAGTCAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGc < 6:445584/139‑1 (MQ=255) cTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGctc > 5:131247/1‑139 (MQ=255) tCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCtt > 4:118896/1‑138 (MQ=255) attgttgGCATCCCGCTAACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGATATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACa < 5:400056/133‑1 (MQ=255) aaTGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTGGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACa < 7:128465/138‑1 (MQ=255) aaTGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACa < 5:149693/138‑1 (MQ=255) aaTGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACa < 1:19080/138‑1 (MQ=255) aaTGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACa < 4:379368/138‑1 (MQ=255) tCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGCCCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAatat > 3:307815/1‑139 (MQ=255) tCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTgagaga < 6:78421/73‑1 (MQ=255) tCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTgagaga > 5:78421/1‑73 (MQ=255) cTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCgggg < 4:365285/139‑1 (MQ=255) cTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCgggg > 7:311824/1‑139 (MQ=255) cTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGACAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCgggg < 3:392314/139‑1 (MQ=255) gCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCGCTTTACATACCGCAATATCGGGGAAGTCAt > 6:310439/1‑139 (MQ=255) cAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTgaga < 2:51046/139‑1 (MQ=255) cAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTgaga < 6:131247/139‑1 (MQ=255) cAATCAGATCCCAAGCATCACAAAATTGCGCCGACCATTCTCGAACAAGGGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGGCATTgaga < 4:161831/139‑1 (MQ=255) ccAATCATCCCAAAATTGCGCCGACCATTCTCGAACAAGGGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATc < 8:205455/139‑1 (MQ=255) ccAATCATCACAAAATTGCGCCGACCATTCTCGACCAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATc < 2:247387/139‑1 (MQ=255) ccAATCATCACAAAATTGCGCCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATc < 3:118896/139‑1 (MQ=255) atcatcACAAAATTGCGCCGCCCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATcgcg > 8:73687/1‑139 (MQ=255) catcaCAAAATTGCGCCGACCATTCTCGAACAAGCGCGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGcc < 5:402843/139‑1 (MQ=255) acaAAATTGCGCCCACCATTCTCGAACAAGGGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTaa < 2:109927/139‑1 (MQ=255) aaTTGCGCCGACGATTCTCGACATAGTGCGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCGCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCg < 1:80902/139‑1 (MQ=255) tGCGCGGTCCATTCTCGACCAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATATTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCt < 7:313587/138‑1 (MQ=255) cgcCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttctt < 7:370848/71‑1 (MQ=255) cgcCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGAttctt > 8:370848/1‑71 (MQ=255) cgcCGACCATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCg < 1:239756/138‑1 (MQ=255) cATTCTCGAACAGGTGTGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCGCCCTGTAt < 4:3969/139‑1 (MQ=255) cATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCGCCCTGTAt < 8:134591/139‑1 (MQ=255) cATTCTCGAACAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCGCCCTGTAt < 8:146668/139‑1 (MQ=255) cAAGTGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTGTACATACCGCAATATCGGGGAAGTCTTTGAGAAAGTCCAATCGCGCCCTATGCCGCTCGCGCTGGTTCTCTTcacct > 3:320973/1‑138 (MQ=255) | ACAGTACGGCAAAATCGTCAGTGAGCGCCACTATCAGCGGCTGCTCAGCTTTCTGAATGATGGCATCCCGCTCACAGGCGGACAATCTGATCCCAATCATCACAAAATTGCGCCGACCATTCTCGAACAAATGAGAGATGACTCTCCAGTGATGCAGGAGGAGATTTTCGGGCCGATTCTTCCGCTCTTTACATACCGCAATATCGGGGAAGTCATTGAGAAAGTCCAATCGCGCCCTAAGCCGCTCGCCCTGTATCTCTTCACCA > CP006881/3714984‑3715249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |