Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,721,510 T→C R459R (AGA→AGG)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,721,5100TC98.0% 157.8 / NA 50R459R (AGA→AGG) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/32);  minor base A (1/0);  total (18/32)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGTCATTCAGCAGCCCAACCGGCGGCATGATGTGAAAATGCTGGCGGTGCGGATCGCTATTAGCAATAGGTTCATTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACC  >  CP006881/3721374‑3721644
                                                                                                                                        |                                                                                                                                      
gggTCATTCAGCAGCCCACCCGGCGGCATGATATGAAAATGCGGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTcct                                                                                                                                      <  8:376547/139‑1 (MQ=255)
gggTCATTCAGCAGCCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTcct                                                                                                                                      <  4:202399/139‑1 (MQ=255)
     aTTCAGCAGCCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTctcct                                                                                                                                 >  1:64643/1‑139 (MQ=255)
     aTTCAGCAGCCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTctcct                                                                                                                                 >  4:136738/1‑139 (MQ=255)
       tCAGCAGCCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctca                                                                                                                               >  6:75549/1‑139 (MQ=255)
         aggagcCCCACCGGATGCATGATATGAAAATGCTGGCGGTGCGGAGCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatc                                                                                                                             <  5:16432/136‑1 (MQ=255)
         agcatcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAATCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcat                                                                                                                              <  5:420541/138‑1 (MQ=255)
         agcagcCCCAGCGTAGGCTTGTTATGATTATGGTTGCGGTGCTGATCGCTGTTAGTGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACTAAGCTCCTTGTCATGTGCTGTCATTTTTTCCTCtcctcatc                                                                                                                             <  5:16078/139‑1 (MQ=255)
         agcagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatc                                                                                                                             <  1:317904/139‑1 (MQ=255)
         agcagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatc                                                                                                                             <  7:389684/139‑1 (MQ=255)
         agcagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatc                                                                                                                             <  4:428439/139‑1 (MQ=255)
         agcagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcat                                                                                                                              <  5:4071/138‑1 (MQ=255)
           cagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatcct                                                                                                                           <  7:113075/139‑1 (MQ=255)
           cagcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGAGCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatcct                                                                                                                           <  8:95534/139‑1 (MQ=255)
             gcCCAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCtcctcatcctca                                                                                                                         <  2:359921/139‑1 (MQ=255)
              cccAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGACGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTcctcatcctcat                                                                                                                        >  7:211570/1‑139 (MQ=255)
              cccAACCGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTcctcctcctcat                                                                                                                        >  2:277768/1‑139 (MQ=255)
                   ccGGCGGCATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATc                                                                                                                   >  4:182650/1‑139 (MQ=255)
                      gcggcATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAatcatc                                                                                                                <  2:317928/139‑1 (MQ=255)
                        ggcATGATATGAAAATTCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTACTCTCCTCATCCTCATTACTCTTCAt                                                                                                              >  3:344498/1‑139 (MQ=255)
                        ggcATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCAt                                                                                                              <  6:23759/139‑1 (MQ=255)
                        ggcATGATATGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCAt                                                                                                              >  8:210406/1‑139 (MQ=255)
                           atgataTGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGac                                                                                                           <  8:287180/139‑2 (MQ=255)
                           atgataTGAAAATGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGac                                                                                                           <  5:44660/139‑2 (MQ=255)
                                      aTGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaa                                                                                                <  6:237998/139‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTTGTAGCGATGGGGTCTTTTTTCTACACTTCTTCATAAGCCCCGCGTCGTACCTCCTGGGGAAGTGCCGGCAATTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              <  6:375368/138‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTc                                                                                                                <  7:69466/120‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTc                                                                                                                >  8:69466/1‑120 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              <  7:292361/138‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              <  4:31646/138‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              <  8:166826/138‑1 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              >  7:166826/1‑138 (MQ=255)
                                         cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTATCTCCACTTCATCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                              <  6:201576/138‑1 (MQ=255)
                                                              aGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCg                                                                        >  5:302128/1‑139 (MQ=255)
                                                                          ttttttCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAAt                                                              <  2:64643/136‑1 (MQ=255)
                                                                             tttCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGa                                                          <  1:421317/138‑1 (MQ=255)
                                                                                      ttcttcATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGtgaatc                                                 >  2:75640/1‑133 (MQ=255)
                                                                                                gCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                        >  1:312697/1‑135 (MQ=255)
                                                                                                            aGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGgagc                          <  5:75549/139‑1 (MQ=255)
                                                                                                                 atggtCATGTGCTGTCATTTTTTCCTCTCCTAATCCTCATTAATCTTCATGACAAATGCGGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGATTCAAGTTGCTGAAGCCAAACGGAGCCGCaa                     <  8:211570/135‑1 (MQ=255)
                                                                                                                        tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGACCGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGat              >  4:120796/1‑139 (MQ=255)
                                                                                                                        tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGt                                                      >  1:419116/1‑99 (MQ=255)
                                                                                                                        tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGt                                                      <  2:419116/99‑1 (MQ=255)
                                                                                                                        tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                        >  1:380408/1‑111 (MQ=255)
                                                                                                                        tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                        <  2:380408/113‑3 (MQ=255)
                                                                                                                           gCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGatcat           <  6:302128/139‑1 (MQ=255)
                                                                                                                               tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg        >  4:350975/1‑138 (MQ=255)
                                                                                                                               tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg        >  1:178829/1‑138 (MQ=255)
                                                                                                                                 aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt     <  1:67595/139‑1 (MQ=255)
                                                                                                                                 aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt     <  2:178829/139‑1 (MQ=255)
                                                                                                                                 aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt     <  7:347912/139‑1 (MQ=255)
                                                                                                                                 aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt     <  3:350975/139‑1 (MQ=255)
                                                                                                                                    tttttCTCTCCTCATCATCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACAAGGTTGCCAAAGCCAAACGGCCCCGCAATAGCGATCATCGGAATAcc  >  8:85726/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                                      
GGGTCATTCAGCAGCCCAACCGGCGGCATGATGTGAAAATGCTGGCGGTGCGGATCGCTATTAGCAATAGGTTCATTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACC  >  CP006881/3721374‑3721644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑