Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,721,539 T→C I450V (ATC→GTC)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,721,5390TC96.2% 159.9 / ‑3.7 53I450V (ATC→GTC) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (23/28);  total (24/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGCTGGCGGTGCGGATCGCTATTAGCAATAGGTTCATTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACAT  >  CP006881/3721412‑3721673
                                                                                                                               |                                                                                                                                      
aTGCTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaa                                                                                                                             <  6:237998/139‑1 (MQ=255)
   cTGGCGGTGCGGATCGCTTGTAGCGATGGGGTCTTTTTTCTACACTTCTTCATAAGCCCCGCGTCGTACCTCCTGGGGAAGTGCCGGCAATTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           <  6:375368/138‑1 (MQ=255)
   cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           <  4:31646/138‑1 (MQ=255)
   cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           >  7:166826/1‑138 (MQ=255)
   cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           <  7:292361/138‑1 (MQ=255)
   cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           <  8:166826/138‑1 (MQ=255)
   cTGGCGGTGCGGATCGCTGTTAGCGATGGGCTCTTTTATCTCCACTTCATCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCaatg                                                                                                                           <  6:201576/138‑1 (MQ=255)
                        aGCGATGGGCTCTTTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCg                                                                                                     >  5:302128/1‑139 (MQ=255)
                                    ttttttCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAAt                                                                                           <  2:64643/136‑1 (MQ=255)
                                       tttCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGa                                                                                       <  1:421317/138‑1 (MQ=255)
                                                ttcttcATAAGCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGtgaatc                                                                              >  2:75640/1‑133 (MQ=255)
                                                          gCCCGGCGACGAAGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                                                     >  1:312697/1‑135 (MQ=255)
                                                                      aGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGgagc                                                       <  5:75549/139‑1 (MQ=255)
                                                                           atggtCATGTGCTGTCATTTTTTCCTCTCCTAATCCTCATTAATCTTCATGACAAATGCGGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGATTCAAGTTGCTGAAGCCAAACGGAGCCGCaa                                                  <  8:211570/135‑1 (MQ=255)
                                                                                  tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGACCGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGat                                           >  4:120796/1‑139 (MQ=255)
                                                                                  tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGt                                                                                   <  2:419116/99‑1 (MQ=255)
                                                                                  tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGt                                                                                   >  1:419116/1‑99 (MQ=255)
                                                                                  tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                                                     <  2:380408/113‑3 (MQ=255)
                                                                                  tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTgctg                                                                     >  1:380408/1‑111 (MQ=255)
                                                                                     gCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGatcat                                        <  6:302128/139‑1 (MQ=255)
                                                                                         tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg                                     >  1:178829/1‑138 (MQ=255)
                                                                                         tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg                                     >  4:350975/1‑138 (MQ=255)
                                                                                           aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt                                  <  7:347912/139‑1 (MQ=255)
                                                                                           aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt                                  <  3:350975/139‑1 (MQ=255)
                                                                                           aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt                                  <  2:178829/139‑1 (MQ=255)
                                                                                           aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt                                  <  1:67595/139‑1 (MQ=255)
                                                                                              tttttCTCTCCTCATCATCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACAAGGTTGCCAAAGCCAAACGGCCCCGCAATAGCGATCATCGGAATAcc                               >  8:85726/1‑139 (MQ=255)
                                                                                                  cctctcCTCCTCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCAAGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGt                            <  2:301399/137‑1 (MQ=255)
                                                                                                   ctctcCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGtt                           <  2:312697/138‑1 (MQ=255)
                                                                                                   ctctcCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTa                          <  3:417028/139‑1 (MQ=255)
                                                                                                      tcctcatcctcaTTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCa                                                                                                         >  5:148578/1‑57 (MQ=255)
                                                                                                      tcctcatcctcaTTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCa                                                                                                         <  6:148578/57‑1 (MQ=255)
                                                                                                           atcctcatTAATCTTCATGACAAATGCTGCAAGGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTaa                  <  4:23353/139‑1 (MQ=255)
                                                                                                           atcctcatTAATCTTCATGACAAAACCTGCAATGAACCCTGACACTGCCGCAATCGCCAAGCCAATCAGATAGTCAATCAAGTTGCTGAAGCCAAACGGAGCCCCAATGGCGATCATCGGAATTCCCGATAACCCGt                    >  7:56246/1‑137 (MQ=255)
                                                                                                             cctcatTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCTTCGGAATTCCCGTTAACCCGTCAGc                >  2:372314/1‑139 (MQ=255)
                                                                                                                catTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAACCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt             >  8:216368/1‑139 (MQ=255)
                                                                                                                catTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt             >  5:24480/1‑139 (MQ=255)
                                                                                                                 atTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt             <  5:194748/138‑1 (MQ=255)
                                                                                                                  ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCAt                                                                                                 <  6:114668/53‑1 (MQ=255)
                                                                                                                  ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCAt                                                                                                 >  5:114668/1‑53 (MQ=255)
                                                                                                                  ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTg           >  4:147930/1‑139 (MQ=255)
                                                                                                                  ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTg           >  3:398776/1‑139 (MQ=255)
                                                                                                                     aTCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGAt                                                                                      >  7:137447/1‑61 (MQ=255)
                                                                                                                     aTCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGAt                                                                                      <  8:137447/61‑1 (MQ=255)
                                                                                                                          cATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAAc    <  7:85726/138‑1 (MQ=255)
                                                                                                                          cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc    <  6:8831/138‑1 (MQ=255)
                                                                                                                          cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc    <  3:182650/138‑1 (MQ=255)
                                                                                                                          cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACa   >  3:376000/1‑139 (MQ=255)
                                                                                                                          cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACa   >  5:71135/1‑139 (MQ=255)
                                                                                                                           aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc    <  7:260576/137‑1 (MQ=255)
                                                                                                                           aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc    >  8:260576/1‑137 (MQ=255)
                                                                                                                           aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACAt  >  4:361073/1‑139 (MQ=255)
                                                                                                                           aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCAATGACAATCTGATAGTGAATCAAGATGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCAGTTAACCCGTAAGCGTTTGCAGCTACAt  >  4:362225/1‑139 (MQ=255)
                                                                                                                               |                                                                                                                                      
ATGCTGGCGGTGCGGATCGCTATTAGCAATAGGTTCATTTTTCTCCACTTCTTCATAAGCCCGGCGACGAAGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACAT  >  CP006881/3721412‑3721673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑