| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,721,618 | C→A | A423A (GCG→GCT) | U712_19190 ← | PTS system sucrose‑specific EIIBC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,721,618 | 0 | C | A | 96.6% | 179.5 / ‑3.9 | 59 | A423A (GCG→GCT) | U712_19190 | PTS system sucrose‑specific EIIBC component |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (28/29); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
AGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACATGTGTAAAGACGACATATGCACCGCCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGAAGA > CP006881/3721482‑3721747 | aGCTCCTGGTCATGTGCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGgagc < 5:75549/139‑1 (MQ=255) atggtCATGTGCTGTCATTTTTTCCTCTCCTAATCCTCATTAATCTTCATGACAAATGCGGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGATTCAAGTTGCTGAAGCCAAACGGAGCCGCaa < 8:211570/135‑1 (MQ=255) tgtgCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGACCGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGat > 4:120796/1‑139 (MQ=255) gCTGTCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGatcat < 6:302128/139‑1 (MQ=255) tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg > 1:178829/1‑138 (MQ=255) tCATTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCgg > 4:350975/1‑138 (MQ=255) aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt < 1:67595/139‑1 (MQ=255) aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt < 2:178829/139‑1 (MQ=255) aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt < 7:347912/139‑1 (MQ=255) aTTTTTTCCTCTCCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAAt < 3:350975/139‑1 (MQ=255) tttttCTCTCCTCATCATCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACAAGGTTGCCAAAGCCAAACGGCCCCGCAATAGCGATCATCGGAATAcc > 8:85726/1‑139 (MQ=255) cctctcCTCCTCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCAAGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGt < 2:301399/137‑1 (MQ=255) ctctcCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGtt < 2:312697/138‑1 (MQ=255) ctctcCTCATCCTCATTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTa < 3:417028/139‑1 (MQ=255) atcctcatTAATCTTCATGACAAATGCTGCAAGGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTaa < 4:23353/139‑1 (MQ=255) atcctcatTAATCTTCATGACAAAACCTGCAATGAACCCTGACACTGCCGCAATCGCCAAGCCAATCAGATAGTCAATCAAGTTGCTGAAGCCAAACGGAGCCCCAATGGCGATCATCGGAATTCCCGATAACCCGt > 7:56246/1‑137 (MQ=255) cctcatTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCTTCGGAATTCCCGTTAACCCGTCAGc > 2:372314/1‑139 (MQ=255) catTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAACCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt > 8:216368/1‑139 (MQ=255) catTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt > 5:24480/1‑139 (MQ=255) atTAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGtt < 5:194748/138‑1 (MQ=255) ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTg > 3:398776/1‑139 (MQ=255) ttAATCTTCATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTg > 4:147930/1‑139 (MQ=255) cATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAAc < 7:85726/138‑1 (MQ=255) cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc < 3:182650/138‑1 (MQ=255) cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc < 6:8831/138‑1 (MQ=255) cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACa > 3:376000/1‑139 (MQ=255) cATGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACa > 5:71135/1‑139 (MQ=255) aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc > 8:260576/1‑137 (MQ=255) aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTAc < 7:260576/137‑1 (MQ=255) aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACAt > 4:361073/1‑139 (MQ=255) aTGACAAATGCTGCAATGAACGCTGACACTGCCGCAATCGCAATGACAATCTGATAGTGAATCAAGATGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCAGTTAACCCGTAAGCGTTTGCAGCTACAt > 4:362225/1‑139 (MQ=255) atgCTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTa > 4:49590/3‑137 (MQ=255) cTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCTTTTGCAGCTACATGTGTAAAga < 4:398776/138‑1 (MQ=255) cTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAga < 5:160852/138‑1 (MQ=255) cTGCAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAga < 3:136738/138‑1 (MQ=255) cAATGAACGCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAgacga > 8:325387/1‑138 (MQ=255) aatgaacgCTGACACTGCCGCAAGCGCCATGCCAATCAGATCGTGCAGCAAGTTGGTTACCCCAAACGGAGCCGCAATGGCGATCAGCGGAATGCCCGTTAACCCGTATGCGTTTGAAGATACATGTGTAAAGACGACa < 4:344498/139‑1 (MQ=255) atgaacgCTGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACat < 7:325387/139‑1 (MQ=255) tGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTgc < 7:89466/108‑1 (MQ=255) tGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTgc > 8:82858/1‑108 (MQ=255) tGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTgc < 7:82858/108‑1 (MQ=255) tGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTgc > 8:89466/1‑108 (MQ=255) tGACACTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGcccca > 7:3842/1‑138 (MQ=255) acacTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCAcc < 1:277768/139‑1 (MQ=255) acacTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCGGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCAcc < 1:99513/139‑1 (MQ=255) cTGCCGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCaa > 6:425052/1‑139 (MQ=255) ccGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGcc < 3:181794/128‑2 (MQ=255) ccGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGcc > 4:181794/1‑127 (MQ=255) ccGCAATCGCCATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATgc > 7:373826/1‑139 (MQ=255) ccATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGat < 5:425052/139‑1 (MQ=255) cATGCCAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGat > 7:155762/1‑138 (MQ=255) ccAATCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATcg < 3:49590/139‑1 (MQ=255) tctgATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATg < 7:20023/117‑1 (MQ=255) tCAGATAGTGAATCAAGTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATg > 8:20023/1‑120 (MQ=255) aatcaagTTGCTGAAGCCAAACGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGt > 7:416758/7‑139 (MQ=255) tgctgaagccaaaCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCTGCGATAAACGGTTTTCgg > 4:59591/6‑137 (MQ=255) tgctgaagccaaaCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCgg > 6:312253/6‑137 (MQ=255) tgctgaagccaaaCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCgg > 3:141256/6‑137 (MQ=255) aagccaacCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTCAGCGTTTGCAGCTAAATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCg < 7:239132/137‑1 (MQ=255) ccaaaaCGGAGCCGCAATGGCGATCATCGGAATTCCCGTTAACCCGTATGCGTTGGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGa < 4:141256/136‑1 (MQ=255) | AGCTCCTGATCATGTGCTGTCATTTTTTTCTCTCCTCATCCTCATTAATCTTCATGATAAACGCTGCAATGAATGCTGACACTGCCGCAATCGCCATTCCAATCAGATAGTTGACCAGGTTGCCAAAGCCAAACGGCGCCGCAATAGCGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACATGTGTAAAGACGACATATGCACCGCCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGAAGA > CP006881/3721482‑3721747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |