Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,230	C→T	E232E (GAG→GAA)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,230	0	C	T	100.0%	107.9 / NA	36	E232E (GAG→GAA)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

AGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACA  >  CP006881/2174102‑2174359                                                                                                                                 |                                                                                                                                  aGCTTCCAGGCTTTCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTAt                                                                                                                         >  1:66109/1‑139 (MQ=255)              tCAGGAAAAGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAg                                                                                                             >  8:34147/1‑138 (MQ=255)                 ggAAAAGGTGCGCGTCTTTCAAAACTATGCAATGGTTAATTTAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGCTTTCTCGTTTTTGATAAAAACCAggaat                                                                                                         <  6:393025/139‑4 (MQ=255)                     aaGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCTAAGCTCTTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                     <  4:34442/139‑2 (MQ=255)                                   tCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatctc                                                                                         <  6:407125/137‑6 (MQ=255)                                            tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                                                           >  1:248901/4‑123 (MQ=255)                                            tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                                                           <  2:248901/123‑4 (MQ=255)                                            tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATg                                                                                <  6:409819/134‑1 (MQ=255)                                            tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                                              >  8:46152/4‑139 (MQ=255)                                            tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                                              >  5:153100/4‑139 (MQ=255)                                                      aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                     <  8:356785/106‑2 (MQ=255)                                                      aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                     >  7:356785/1‑105 (MQ=255)                                                      aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAg                                                                     <  4:94325/138‑1 (MQ=255)                                                            aaGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTgg                                                              >  7:102138/1‑139 (MQ=255)                                                              gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                            <  7:11422/137‑1 (MQ=255)                                                              gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                            >  5:38443/3‑139 (MQ=255)                                                              gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                            <  6:100129/137‑1 (MQ=255)                                                                gcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCgag                                                          <  7:19512/139‑3 (MQ=255)                                                                              ctCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGTTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTATGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTg                                             >  6:275786/2‑138 (MQ=255)                                                                                    ggATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTgcagcaat                                      <  4:241331/139‑3 (MQ=255)                                                                                        gTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTtc                                   >  5:401310/1‑138 (MQ=255)                                                                                           aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                >  7:232617/1‑138 (MQ=255)                                                                                           aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                <  8:88562/138‑1 (MQ=255)                                                                                           aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                <  6:318709/138‑1 (MQ=255)                                                                                            aaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCaaa                              <  5:114376/139‑1 (MQ=255)                                                                                                 aagaatggAATCTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                         >  7:126220/7‑139 (MQ=255)                                                                                                 aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAACCTCTa                         >  5:97589/7‑139 (MQ=255)                                                                                                 aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                         >  7:350820/7‑139 (MQ=255)                                                                                                 aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                         >  1:227551/7‑139 (MQ=255)                                                                                                   gaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                         >  7:383888/5‑137 (MQ=255)                                                                                                      tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                <  3:318643/126‑1 (MQ=255)                                                                                                      tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCATGGCGAGAAGCTCTTACCTGCAGCAATTTCTCa                                >  4:318643/2‑127 (MQ=255)                                                                                                              cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc            >  3:293236/1‑139 (MQ=255)                                                                                                              cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc            >  2:432906/1‑139 (MQ=255)                                                                                                                        aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa  <  7:427466/133‑1 (MQ=255)                                                                                                                        aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa  <  2:66109/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                  AGCTTCCATGCTTTCAGGAAAAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACA  >  CP006881/2174102‑2174359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑