Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,721,765 T→G P374P (CCA→CCC)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,721,7650TG98.1% 162.9 / NA 52P374P (CCA→CCC) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (31/20);  total (31/21)
Fisher's exact test for biased strand distribution p-value = 4.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACATGTGTAAAGACGACATATGCACCGCCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGAAGATTGACTCCGAATATGACTGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCAGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTCTTCGCCATAAAGAAGACGGCAAGGCCGGCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGC  >  CP006881/3721629‑3721897
                                                                                                                                        |                                                                                                                                    
cGATCATCGGAATTCCCGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACggg                                                                                                                                    <  3:361073/139‑1 (MQ=255)
cGATCATCGGAATTCCCGTTAACCCGGAAGCGTTTCCAGCTACTTGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACggg                                                                                                                                    <  1:75640/139‑1 (MQ=255)
               ccGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCCCCCAATGCGCCTCCGAGCAGCGCGGCGATAAACGGTTTTCGGGAGCGCAGATTGCCTCCGAATATGACGGGCTCAGTAATGcagc                                                                                                                      >  8:251871/1‑135 (MQ=255)
               ccGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACggg                                                                                                                                    >  8:57282/1‑124 (MQ=255)
               ccGTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACggg                                                                                                                                    <  7:57282/124‑1 (MQ=255)
                 gTTAACCCGTAAGCGTTTGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCgaga                                                                                                                    >  7:398754/1‑138 (MQ=255)
                                tttGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGctg                                                                                                    >  1:224857/1‑137 (MQ=255)
                                 ttGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAAtgctgc                                                                                                   >  1:258969/1‑139 (MQ=255)
                                 ttGCAGCTACATGTGTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGACGAGAAAAGCGGAAAAtgctgc                                                                                                   >  6:4991/1‑139 (MQ=255)
                                          cATGTGAAACGACGACATCTGCCCCACCCACTGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAgcgc                                                                                          <  8:373826/139‑1 (MQ=255)
                                            tgtgTACAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGa                                                                                        <  3:120796/139‑1 (MQ=255)
                                            tgtgTAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGa                                                                                        <  5:312253/139‑1 (MQ=255)
                                             gtgtAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGa                                                                                        <  3:59591/138‑1 (MQ=255)
                                              tgtAAAGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGAt                                                                                       <  5:138226/138‑1 (MQ=255)
                                                  aaGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCtt                                                                                  >  8:288622/1‑139 (MQ=255)
                                                  aaGACGACATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCtt                                                                                  <  8:379805/139‑1 (MQ=255)
                                                        acATATGCCCCACCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCt                                                                            >  4:259744/1‑139 (MQ=255)
                                                              gccccacccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGGct                                                                      >  7:324082/7‑139 (MQ=255)
                                                                   acccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTTTCTTTGCctttttg                                                                 >  1:298536/2‑138 (MQ=255)
                                                                   acccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCtttttc                                                                 >  1:144263/2‑139 (MQ=255)
                                                                    cccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGAt                                                                                       <  3:425983/116‑1 (MQ=255)
                                                                    cccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGAt                                                                                       >  4:425983/1‑116 (MQ=255)
                                                                    cccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCg                                                                >  4:440938/1‑139 (MQ=255)
                                                                    cccAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCGTTTTCg                                                                >  3:33641/1‑139 (MQ=255)
                                                                    accAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTAGGTAGCGCAGAAAGACTCCGAAAATGACGGGCTCAGGAATGCAGAGAAAAGCGGAAAATGCTGCCGGAACCGCGCTTTCATTTGTCTTGGCCTTTTTCg                                                                >  7:332437/2‑139 (MQ=255)
                                                                     ccAATGCGCCGCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGc                                                               <  8:416758/139‑1 (MQ=255)
                                                                         tGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCGAt                                                            >  5:31421/1‑138 (MQ=255)
                                                                         tGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCAt                                                            >  6:3741/1‑138 (MQ=255)
                                                                         tGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCAt                                                            >  5:395033/1‑138 (MQ=255)
                                                                              cTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGTTTTCGTTTGTCGTTGCCTTTTTAGCCATAaacaa                                                      >  1:222448/1‑139 (MQ=255)
                                                                              cTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGACATAaag                                                        >  3:96523/1‑137 (MQ=255)
                                                                               gccGTTCATCGCGGCGATAAACGGTTTTCGGTAGCGCCGAGTCACTCCGAATCTGACGGGCTCAGTAATGCCGTGAAAAGCGGAAAAGGCTGCCGGACGCGCTTTTTCTTTTGGGTTTGCCTTTTTCGCCATaaccaag                                                     <  2:258969/138‑6 (MQ=255)
                                                                                      atcGCGGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGc                                                >  5:87310/1‑137 (MQ=255)
                                                                                        cgcgGCGATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAga                                            >  1:56406/1‑138 (MQ=255)
                                                                                         ggggctATAATCTGTTTTCGGTAGCGTATATTGACTCCTAATTTGTCTTGCTCATTAATCCCGAGACAAGCGGAAAATGCTGCGGGAAGCGGGATTTCTTTTGTCTTTGCATTTTTCGCCATAAATAACACGGCAAgac                                           <  8:332437/137‑3 (MQ=255)
                                                                                         gcggcgATAACCGGTTTGCGGTCGCGCATCTTGACTCCGAATATGACGGGCTCAGTCATGCCGAGAACAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           <  5:403909/139‑3 (MQ=255)
                                                                                         gcggcgATAAACGGTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           <  7:288622/139‑3 (MQ=255)
                                                                                              gATAAACGTTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTAAGTACTTCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGcc                                      <  6:71135/139‑2 (MQ=255)
                                                                                                   aCGGTTTTCGGTAGCGCAGATTGACTCCGAATATGCCGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGcc                                 <  8:3842/139‑1 (MQ=255)
                                                                                                       ttttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGTTTTGTTTTTTCTTTGCCTTTTTGGCCATAAATAATACCGCAAGACCGGgcccgccccgg                             >  1:273468/1‑136 (MQ=255)
                                                                                                       ttttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           <  8:356796/125‑3 (MQ=255)
                                                                                                       ttttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           >  7:356796/1‑123 (MQ=255)
                                                                                                         ttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGCTTTCTTTTGTCTTTGGCTTTTTGGCCATAAAGAAGACCGCAAGACCTGCCCCGCCCGGTGc                           >  5:296932/1‑139 (MQ=255)
                                                                                                         ttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGc                           >  3:159820/1‑139 (MQ=255)
                                                                                                                gcgctgATTGCCTCCGAATATGAGGGGCTCAGTAAGGCCGAGAAAAGCGGAAAATGCTGCGGGCAGCGCGATTTCTTTTGGCTTTGCCTTTTTCGCAATAAAGATGACGGCAAGACCTGCCCCGCCCTGTGCGACAtt                     <  3:259744/133‑1 (MQ=255)
                                                                                                                 cgcaGATTGACTCCGAATATGCCGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACCGCAAGACCTGCCCCGCCCTGTGCGCCATTCg                   >  3:90831/4‑139 (MQ=255)
                                                                                                                 cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAACGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCTTAAAGACGACGGCCAGACCTGCCCCGCCCTGTGCGACATTCg                   >  5:12228/4‑139 (MQ=255)
                                                                                                                 cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGGCATAAATAAGACGGCAAGACCTGCCCCGCCCTGTGGGACATTCg                   >  3:84478/4‑139 (MQ=255)
                                                                                                                 cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCg                   >  3:419623/4‑139 (MQ=255)
                                                                                                                    aGATTGACTCCGAATCTGACGGGCTCAGTAATGCCGAGAAACGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCa                <  4:376000/139‑1 (MQ=255)
                                                                                                                          aCTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGAc           <  6:395033/138‑1 (MQ=255)
                                                                                                                              ccaaTAGGACGGCCTCAGTACTGCCGAGAAACGCGGAAACTGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGACGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGAt      <  8:155762/137‑1 (MQ=255)
                                                                                                                              cGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           >  8:122489/1‑100 (MQ=255)
                                                                                                                              cGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAgac                                           <  7:122489/102‑3 (MQ=255)
                                                                                                                              cGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGAc           >  4:204655/1‑134 (MQ=255)
                                                                                                                                  tatGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAAGGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGgc  <  3:147930/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                    
CGATCATCGGAATACCCGTTAACCCGTAAGCGTTTGCAGCAACATGTGTAAAGACGACATATGCACCGCCCAATGCGCCTCCGATCATCGCGGCGATAAACGGTTTTCGGTAGCGAAGATTGACTCCGAATATGACTGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCAGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTCTTCGCCATAAAGAAGACGGCAAGGCCGGCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGC  >  CP006881/3721629‑3721897

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑