Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,721,855 G→A G344G (GGC→GGT)  U712_19190 ← PTS system sucrose‑specific EIIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,721,8550GA98.2% 146.4 / NA 56G344G (GGC→GGT) U712_19190PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (23/31);  minor base C (1/0);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATAAACGGTTTTCGGTAGCGAAGATTGACTCCGAATATGACTGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCAGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTCTTCGCCATAAAGAAGACGGCAAGGCCGGCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATAAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAAAACGATAAGTGAATAT  >  CP006881/3721723‑3721987
                                                                                                                                    |                                                                                                                                    
gATAAACGTTTTTCGGTAGCGCAGATTGACTCCGAATATGACGGGCTAAGTACTTCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGcc                                                                                                                                <  6:71135/139‑2 (MQ=255)
     aCGGTTTTCGGTAGCGCAGATTGACTCCGAATATGCCGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGcc                                                                                                                           <  8:3842/139‑1 (MQ=255)
         ttttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGTTTTGTTTTTTCTTTGCCTTTTTGGCCATAAATAATACCGCAAGACCGGgcccgccccgg                                                                                                                       >  1:273468/1‑136 (MQ=255)
           ttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGCTTTCTTTTGTCTTTGGCTTTTTGGCCATAAAGAAGACCGCAAGACCTGCCCCGCCCGGTGc                                                                                                                     >  5:296932/1‑139 (MQ=255)
           ttCGGTAGCGCAGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGc                                                                                                                     >  3:159820/1‑139 (MQ=255)
                  gcgctgATTGCCTCCGAATATGAGGGGCTCAGTAAGGCCGAGAAAAGCGGAAAATGCTGCGGGCAGCGCGATTTCTTTTGGCTTTGCCTTTTTCGCAATAAAGATGACGGCAAGACCTGCCCCGCCCTGTGCGACAtt                                                                                                               <  3:259744/133‑1 (MQ=255)
                   cgcaGATTGACTCCGAATATGCCGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACCGCAAGACCTGCCCCGCCCTGTGCGCCATTCg                                                                                                             >  3:90831/4‑139 (MQ=255)
                   cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAACGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCTTAAAGACGACGGCCAGACCTGCCCCGCCCTGTGCGACATTCg                                                                                                             >  5:12228/4‑139 (MQ=255)
                   cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGGCATAAATAAGACGGCAAGACCTGCCCCGCCCTGTGGGACATTCg                                                                                                             >  3:84478/4‑139 (MQ=255)
                   cgcaGATTGACTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCg                                                                                                             >  3:419623/4‑139 (MQ=255)
                      aGATTGACTCCGAATCTGACGGGCTCAGTAATGCCGAGAAACGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCa                                                                                                          <  4:376000/139‑1 (MQ=255)
                            aCTCCGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGAc                                                                                                     <  6:395033/138‑1 (MQ=255)
                                ccaaTAGGACGGCCTCAGTACTGCCGAGAAACGCGGAAACTGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGACGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGAt                                                                                                <  8:155762/137‑1 (MQ=255)
                                cGAATATGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGAc                                                                                                     >  4:204655/1‑134 (MQ=255)
                                    tatGACGGGCTCAGTAATGCCGAGAAAAGCGGAAAAGGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGgc                                                                                            <  3:147930/139‑1 (MQ=255)
                                          gggCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAag                                                                                      <  6:87310/138‑1 (MQ=255)
                                          aggCTCAGTAATCCCGTGAAATCGGCTAATTGCTGCGGGATCCGCGATTTCTTTTGTCTTTGCCTTTTTCGCAATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAag                                                                                      <  5:416007/138‑1 (MQ=255)
                                            gCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCg                                                                                                                             <  2:24369/98‑1 (MQ=255)
                                            gCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCg                                                                                                                             >  1:24369/1‑98 (MQ=255)
                                            gCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAag                                                                                      >  3:311556/1‑137 (MQ=255)
                                            gCTCAGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTATTTGGCTTTTTCGGCATAAAGACGCCGGCAAGACCTGCCCCGGCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAagtt                                                                                    >  4:106799/1‑138 (MQ=255)
                                                aGTAATGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGttt                                                                                <  4:311556/139‑1 (MQ=255)
                                                     tGCCGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGcc                                                                           >  1:151260/1‑139 (MQ=255)
                                                       ccGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGcc                                                                                                                           <  6:284268/89‑1 (MQ=255)
                                                       ccGAGAAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGcc                                                                                                                           >  5:284268/1‑89 (MQ=255)
                                                       ccGAGAAAAGCGGAAAATGCTGCCGCAAGCGCGATTTCTTTTGTCTTTGCCTTTTTAGCCATAAAGAAGACGGGAAGACCTGCCCCGCCCTGTGCGAAAAACGACATTGACCAGCTACGCAGCAAGTAGtttttagccg                                                                         >  6:416007/1‑134 (MQ=255)
                                                          agaAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTTTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTatat                                                                      >  2:52392/1‑139 (MQ=255)
                                                          agaAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTTCCTTTTTCGCCATAAAGAAGACGGCACTACCTGCCCAGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCtata                                                                       >  5:95850/1‑138 (MQ=255)
                                                          agaAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACAtt                                                                                                               <  6:219074/98‑1 (MQ=255)
                                                          agaAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACAtt                                                                                                               >  5:219074/1‑98 (MQ=255)
                                                          agaAAAGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTatat                                                                      >  5:145776/1‑139 (MQ=255)
                                                            aaaaGCGGAAAATGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCGTTTTCGCCATAAAGAAGACGGCAAGCCCTGCCCCGCCCGGGGCGCCATTCGCCATTGACCAGATCGGCAGCAAGGAGGTTTTGCCTATATCt                                                                    >  6:258108/1‑139 (MQ=255)
                                                            aaaaGCGGAAAATGAGGCCGGAAGAGCGATTTATTTTGTCTTTGCCTTTTTCGCCATAAAGACGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCt                                                                    <  7:251871/139‑1 (MQ=255)
                                                                 ctgAAAATGCTGCCGGAAGCGAGATTTCTTTTGTCTTTGCCTTTTTCGCCATACTGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAAt                                                               <  8:324082/137‑1 (MQ=255)
                                                                 cGGAAACAGCTGCCGGAAGCGCGAGGTCTTTTGTCTTTGCCTTTTTCGCCAAAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAAt                                                               <  4:84478/139‑1 (MQ=255)
                                                                 cGGAAAATGCTGCCGGAAGCGCGATTTGTTTTGTCTTTGCCTTTTTCCCAAAACACAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAAt                                                               <  4:90831/139‑1 (MQ=255)
                                                                      actgctgcCTGAAGAGCGTTTTATTTTGTGTTTGCCTTTTTCGCCATAACGACCACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGcc                                                          <  6:296932/137‑1 (MQ=255)
                                                                      aaTGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGcc                                                          <  2:144263/139‑1 (MQ=255)
                                                                      aaTGCTGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGcc                                                          <  2:56406/139‑1 (MQ=255)
                                                                      aaTGCGGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGcc                                                          <  4:159820/139‑1 (MQ=255)
                                                                           tgCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGTCCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGcc                                                     >  8:36407/2‑139 (MQ=255)
                                                                                  aGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGAACTGCCCCGCCCTGTGGGACATTCGCCATTGACCAGATCGGCAGCAAGTATTTTTTGCCTATATCTGCAATTATCCCTGCGTCAAACg                                              >  2:232580/1‑139 (MQ=255)
                                                                                  aGCGCGATTTCTTTTGTCTTTGCCTTTTTCGCCATAAAGAAGACAGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCg                                              <  3:440938/139‑1 (MQ=255)
                                                                                  aGCGCGATTTCTTTTGGCTTTGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCg                                              <  8:398754/139‑1 (MQ=255)
                                                                                       gATTTCTTTTGTCTTTGCATTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATgg                                         <  5:3741/139‑1 (MQ=255)
                                                                                           tCTTTTGTCTTTGCCTTTTACGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAg                                      <  4:33641/138‑1 (MQ=255)
                                                                                                    tttGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAAtt                                                              <  2:315391/105‑2 (MQ=255)
                                                                                                    tttGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAAtt                                                              >  1:315391/1‑104 (MQ=255)
                                                                                                      tGCCTTTTTCGCCATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCTACTAGTTTTTGCCTAAATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACg                          >  2:350926/1‑139 (MQ=255)
                                                                                                               cGCAATAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCATCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCag                  <  5:4991/138‑2 (MQ=255)
                                                                                                                  aaTAAAGAAGACGGCAAGACCAGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCagcacg              <  6:31421/138‑6 (MQ=255)
                                                                                                                    tAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGAt            <  6:145776/139‑1 (MQ=255)
                                                                                                                    tAAAGAAGACGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGAt            <  4:419623/139‑1 (MQ=255)
                                                                                                                        gaagaCGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGt        <  4:96523/139‑1 (MQ=255)
                                                                                                                            aCGGCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGaa     >  8:2098/1‑138 (MQ=255)
                                                                                                                              ggCAAGACCTGCCCCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAatac  >  5:269245/1‑138 (MQ=255)
                                                                                                                                    |                                                                                                                                    
GATAAACGGTTTTCGGTAGCGAAGATTGACTCCGAATATGACTGGCTCAGTAATGCCGAGAAAAGCGGAAAATGCAGCCGGAAGCGCGATTTCTTTTGTCTTTGCCTTCTTCGCCATAAAGAAGACGGCAAGGCCGGCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATAAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAAAACGATAAGTGAATAT  >  CP006881/3721723‑3721987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑