Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,721,987	T→C	T300T (ACA→ACG)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,721,987	0	T	C	100.0%	325.0 / NA	98	T300T (ACA→ACG)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (55/43);  total (55/43)

GCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATAAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAAAACGATAAGTGAATATGTGCCCCCGAAAATCAGACCCGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATCCCGGAGCCAAGCGCTCTGCCCAAAGGACCAATCGCGATAAAAGCGACAAATCCAGTGACAATCACT  >  CP006881/3721859‑3722122                                                                                                                                 |                                                                                                                                        gccccGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCTAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                >  5:105213/4‑138 (MQ=255) gccccGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                >  8:85743/4‑138 (MQ=255) gccccGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                >  2:97549/4‑138 (MQ=255) gccccGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                >  3:216558/4‑138 (MQ=255) gccccGCCCTGTGCGACATTCGCCATAGCCCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAAGACGTGCCCCCg                                                                                                                                >  5:374725/4‑138 (MQ=255)  ccccGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGa                                                                                                                               >  7:72779/3‑138 (MQ=255)       cccTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAAt                                                                                                                           >  3:332733/1‑137 (MQ=255)       cccTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAAt                                                                                                                           >  2:121880/1‑137 (MQ=255)            tgCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGAcct                                                                                                                    >  5:342400/1‑138 (MQ=255)            tgCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGAcct                                                                                                                    >  5:145295/1‑138 (MQ=255)            tgCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGAcct                                                                                                                    >  2:160106/1‑138 (MQ=255)                 cATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGa                                                                                                                >  7:445864/1‑138 (MQ=255)                 cATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGa                                                                                                                >  5:433997/1‑138 (MQ=255)                    tCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCACTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACaaa                                                                                                            >  2:118953/1‑139 (MQ=255)                      gCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAAc                                                                                                          >  6:294249/1‑139 (MQ=255)                      gCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAAc                                                                                                          >  5:281752/1‑139 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCTTGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGGGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  7:155688/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  5:258108/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  5:294249/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  8:72779/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  3:204655/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  1:160106/138‑1 (MQ=255)                            gACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  2:298536/138‑1 (MQ=255)                             aCCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCAt                                                                                                    <  1:372314/138‑1 (MQ=255)                                aGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTc                                                                                                <  6:374725/139‑1 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTTCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCa                                                                                               >  3:340727/1‑138 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     >  5:318600/1‑132 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  6:318600/132‑1 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     >  6:309013/1‑132 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  5:309013/132‑1 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCat                                                                                              <  6:433997/139‑1 (MQ=255)                                  aTCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCat                                                                                              <  1:52392/139‑1 (MQ=255)                                       cagcaAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGCCCTGCGACACAACCGGCTTGGTCATATACa                                                                                         >  7:320492/1‑139 (MQ=255)                                       cagcaAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         <  7:2098/139‑1 (MQ=255)                                       cagcaAGTAGTTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         >  4:186904/1‑139 (MQ=255)                                            agtattTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAAGACGTGCCCCCGAAAATCAGCCCTGCGACAAAACCGGCATGGTCATATACATAAGt                                                                                    >  8:12521/1‑139 (MQ=255)                                            agtagtTTTTGCCTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGt                                                                                    >  8:378084/1‑139 (MQ=255)                                                        cTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGt                                                                        <  1:232580/139‑1 (MQ=255)                                                        cTATATCTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGt                                                                        <  4:216558/139‑1 (MQ=255)                                                         tatataTGCAATTAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTa                                                                       <  7:85743/139‑1 (MQ=255)                                                               tGCAATTAGCCTTGAATCAATGGCATGCAAGCTGTGATGAACGCCTGGCTGAAGGATGAGTGAATCCGTGCCCCCGAAAATCATAACTGCGACAAACGGGGCATGGTCATATACATAAGTTAAAGCAACCTTAAtgcgg                                                                 <  6:95850/139‑5 (MQ=255)                                                                      aGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGcc                                                            >  3:341949/1‑137 (MQ=255)                                                                      aGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGcc                                                            >  6:135698/1‑137 (MQ=255)                                                                       gCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         <  1:373153/107‑1 (MQ=255)                                                                       gCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         >  2:373153/1‑107 (MQ=255)                                                                       gCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCaa                                                          <  8:445864/138‑1 (MQ=255)                                                                        cccTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                <  5:333272/67‑1 (MQ=255)                                                                        cccTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCg                                                                                                                                >  6:333272/1‑67 (MQ=255)                                                                           tGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     >  1:393048/1‑139 (MQ=255)                                                                           tGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATCCCGGAGCCACGCGct                                                     >  1:218929/1‑139 (MQ=255)                                                                            gCCTCAATCGCATGGATGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACATGCGACAAAACCGGCATGGTCATATACATTAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGctc                                                    >  6:207522/1‑139 (MQ=255)                                                                            gCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         >  1:279684/1‑102 (MQ=255)                                                                            gCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         <  2:279684/102‑1 (MQ=255)                                                                            gCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAgcgc                                                      >  4:269328/1‑137 (MQ=255)                                                                            gCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGctc                                                    >  3:278938/1‑139 (MQ=255)                                                                            gCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACAGAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGctc                                                    >  5:447323/1‑139 (MQ=255)                                                                             cgtCAATCGCATGGATGATGTGATTATCGCCTGTCAGCACGATGTGTGAATACGTGCCCCGGAAAATCAGACCTGCGACAAAACCGGCTTGGTAATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAgcgcg                                                     <  4:340727/135‑2 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACGTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  1:350926/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  2:393048/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  3:269328/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  7:36407/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  6:145295/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  2:151260/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  5:135698/137‑1 (MQ=255)                                                                             ccTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGct                                                     <  4:341949/137‑1 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  3:371559/84‑1 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     >  4:371559/1‑84 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCa                                                                                               <  7:229619/90‑1 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCa                                                                                               >  2:96309/1‑90 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCa                                                                                               >  8:229619/1‑90 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCa                                                                                               <  1:96309/90‑1 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGcc                                                >  2:174926/1‑137 (MQ=255)                                                                                  aTCGCATGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACATGCGACAAAACCTGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGcc                                                >  3:327961/1‑137 (MQ=255)                                                                                       aTGGAAGCTGTGATGAACGCCTGTCAGCACGATGCGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAggg                                         >  5:223836/1‑138 (MQ=255)                                                                                       aTGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCg                                                                 <  3:291454/115‑1 (MQ=255)                                                                                       aTGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCg                                                                 >  4:291454/1‑115 (MQ=255)                                                                                        tGGAAGCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAgggc                                        >  6:405301/1‑137 (MQ=255)                                                                                             gCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         >  3:242930/1‑85 (MQ=255)                                                                                             gCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACa                                                                                         <  4:242930/85‑1 (MQ=255)                                                                                             gCTGTGATGAACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATc                                   >  4:375536/1‑139 (MQ=255)                                                                                                 tgatgaACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     <  6:242082/69‑1 (MQ=255)                                                                                                 tgatgaACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCa                                                                                                     >  5:242082/1‑69 (MQ=255)                                                                                                 tgatgaACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAAt                                    <  3:286781/134‑1 (MQ=255)                                                                                                 tgatgaACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAAt                                    >  4:286781/1‑134 (MQ=255)                                                                                                     gaACGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATaa                            >  3:416250/1‑138 (MQ=255)                                                                                                        cGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGc                                                                              <  6:417213/85‑1 (MQ=255)                                                                                                        cGCCTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGc                                                                              >  5:417213/1‑85 (MQ=255)                                                                                                           cTGTCAGCACGATGAGTGAATCCGTGCCCCCGAAAAGCAGCCCTGCGACAAAACCGGCAGGGTCATATACATAAGTTATAGCACCCGTAATGCCGGAGCCAAGCGCTCTTCCTAGAGGGCCACTCGCGATAAAAGcaa                      >  5:17016/1‑136 (MQ=255)                                                                                                           cTGTCAGCACGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGcaa                      >  2:336149/1‑136 (MQ=255)                                                                                                             gtcagcaCGATGAGTGAATACGTGCCACCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCCCGATAAAAGCAACa                    >  1:19909/7‑138 (MQ=255)                                                                                                                  caCGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGcaacaaccccg              >  4:409994/2‑134 (MQ=255)                                                                                                                  caCGATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAAcccg              >  1:156969/2‑138 (MQ=255)                                                                                                                     gATGAGTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCTGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCTATAAAAGCAACAAACCCGGTg           <  4:327961/139‑1 (MQ=255)                                                                                                                          gTGAATACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAAt      <  1:97549/139‑1 (MQ=255)                                                                                                                             aaTACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCa    <  3:375536/138‑1 (MQ=255)                                                                                                                             aaTACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCa    >  7:417405/1‑138 (MQ=255)                                                                                                                             aaTACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCa    <  4:416250/138‑1 (MQ=255)                                                                                                                             aaTACGTGCCCCCGAAAATCAGACCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCa    >  5:378209/1‑138 (MQ=255)                                                                                                                              atacGTGCCCCCGAAAATCAGCCCTGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGGAATGCCGGAGCCAAGCGCTCTGCCTAGAGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCAca  <  3:106799/139‑2 (MQ=255)                                                                                                                                 |                                                                                                                                        GCACCGCCCTGTGCGACATTCGCCATTGACCAGATCGGCAGCAAGTAGTTTTTGCCTATATCTGCAATAAGCCCTGCCTCAATCGCATGGAAGCTGTGATGAACGCCTGTCAAAACGATAAGTGAATATGTGCCCCCGAAAATCAGACCCGCGACAAAACCGGCATGGTCATATACATAAGTTAAAGCAACCGTAATCCCGGAGCCAAGCGCTCTGCCCAAAGGACCAATCGCGATAAAAGCGACAAATCCAGTGACAATCACT  >  CP006881/3721859‑3722122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑