Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	2,174,257	T→C	E223E (GAA→GAG)	U712_10875 ←	CCA‑adding enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,174,257	0	T	C	100.0%	114.7 / NA	36	E223E (GAA→GAG)	U712_10875	CCA‑adding enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

AAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACATGGGATAGGAGTGATTTTTCTTTCGTGAT  >  CP006881/2174122‑2174388                                                                                                                                        |                                                                                                                                    aaGGGGCGCGTCTTTCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCTAAGCTCTTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                                                  <  4:34442/139‑2 (MQ=255)               tCAAAACTATGCCAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatctc                                                                                                                      <  6:407125/137‑6 (MQ=255)                        tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                                                                                        >  1:248901/4‑123 (MQ=255)                        tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACaatc                                                                                                                        <  2:248901/123‑4 (MQ=255)                        tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATg                                                                                                             <  6:409819/134‑1 (MQ=255)                        tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                                                                           >  8:46152/4‑139 (MQ=255)                        tgccAAGGTTAATTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAg                                                                                                           >  5:153100/4‑139 (MQ=255)                                  aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                                                  <  8:356785/106‑2 (MQ=255)                                  aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTct                                                                                                                                  >  7:356785/1‑105 (MQ=255)                                  aaTTAAAAGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAg                                                                                                  <  4:94325/138‑1 (MQ=255)                                        aaGAGCAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTgg                                                                                           >  7:102138/1‑139 (MQ=255)                                          gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                         <  7:11422/137‑1 (MQ=255)                                          gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                         >  5:38443/3‑139 (MQ=255)                                          gagcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCg                                                                                         <  6:100129/137‑1 (MQ=255)                                            gcAGCCCAAAGCTCCTCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCgag                                                                                       <  7:19512/139‑3 (MQ=255)                                                          ctCACGGGATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGTTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTATGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTg                                                                          >  6:275786/2‑138 (MQ=255)                                                                ggATGTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTgcagcaat                                                                   <  4:241331/139‑3 (MQ=255)                                                                    gTTAAAGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTtc                                                                >  5:401310/1‑138 (MQ=255)                                                                       aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                             <  8:88562/138‑1 (MQ=255)                                                                       aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                             >  7:232617/1‑138 (MQ=255)                                                                       aaaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                             <  6:318709/138‑1 (MQ=255)                                                                        aaGAGAAGAATGGAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCaaa                                                           <  5:114376/139‑1 (MQ=255)                                                                             aagaatggAATCTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                      >  7:126220/7‑139 (MQ=255)                                                                             aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAACCTCTa                                                      >  5:97589/7‑139 (MQ=255)                                                                             aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                      >  1:227551/7‑139 (MQ=255)                                                                             aagaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                      >  7:350820/7‑139 (MQ=255)                                                                               gaatggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTa                                                      >  7:383888/5‑137 (MQ=255)                                                                                  tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCa                                                             <  3:318643/126‑1 (MQ=255)                                                                                  tggAAACTCACTCGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCATGGCGAGAAGCTCTTACCTGCAGCAATTTCTCa                                                             >  4:318643/2‑127 (MQ=255)                                                                                          cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc                                         >  3:293236/1‑139 (MQ=255)                                                                                          cactcGTTGAAATCAGGTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTc                                         >  2:432906/1‑139 (MQ=255)                                                                                                    aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa                               <  7:427466/133‑1 (MQ=255)                                                                                                    aatcaggTTTTCTCGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACa                               <  2:66109/133‑1 (MQ=255)                                                                                                             ttctcGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGAc                                      <  6:337077/122‑1 (MQ=255)                                                                                                             ttctcGTTTATGATAAAAACCAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGAc                                      >  5:337077/2‑123 (MQ=255)                                                                                                                                 cAGGAAGCTCTTCATACAATCTCGTTTGAATGAGTGTTTGAAGCGCCTGGCGAGAAGCTCTTCCCTGCAGCAATTTCTCAAACTCTATAGTTTTTCGTTCGACTGAAACATGGGAGAGGAGTGATTTTTCTTTCgcaat  <  8:102138/139‑5 (MQ=255)                                                                                                                                        |                                                                                                                                    AAGCGGCGCATCTTTCAAATCTATTTCAAGATCAATTAAAAGCGCAGCCCAAAGCTCTTCACGGGATGTTAAAGAGTAAAACGGAAACTCACTCGCCGCAAGCAAGTTCTCTCGTTTATGATAAAAACCAGGGAGTTCCTCGTACAGCCCCGTTTGAATGAGCGTTTGAAGCGCTTGGCGGGAGGCTCTTCCCTGCAGCATTTTCTCAAACTCTATTGTTTTTCGTTCAACTGAAACATGGGATAGGAGTGATTTTTCTTTCGTGAT  >  CP006881/2174122‑2174388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑