Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,211	A→G	L226L (TTG→CTT)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,211	0	A	G	100.0%	275.6 / NA	84	L226L (TTG→CTT)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (38/46);  total (38/46)

AAGGACCAATCGCGATAAAAGCGACAAATCCAGTGACAATCACTGTAACAAAAGGTGTAACAAGCAAATCTACCGCATGCGGAACTACTTTTCTTGTCCATTTCTCTACCTTGCTCATCACATAAACCGCCAACAAGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGAT  >  CP006881/3722079‑3722343                                                                                                                                     |                                                                                                                                     gaGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  6:397829/138‑1 (MQ=255) gaGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  1:44314/138‑1 (MQ=255) gaGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  1:92806/138‑1 (MQ=255) gaGGGCCAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  7:243239/138‑1 (MQ=255) gaGGGCCAATCGCGAGAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  6:281752/138‑1 (MQ=255)    ggccAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAgg                                                                                                                             <  2:156969/137‑1 (MQ=255)      ccAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGa                                                                                                                           <  5:202458/139‑1 (MQ=255)       cAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCCTCACCTCCACCGCAAGCAGGACAGTGa                                                                                                                           >  6:24192/1‑138 (MQ=255)       cAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGa                                                                                                                           >  2:54248/1‑138 (MQ=255)       cAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGa                                                                                                                           >  1:215886/1‑138 (MQ=255)       cAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGa                                                                                                                           <  4:313936/138‑1 (MQ=255)       cAATCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGa                                                                                                                           >  3:313936/1‑138 (MQ=255)       cAAGCGCGATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGAt                                                                                                                          <  7:228314/139‑1 (MQ=255)         aTCGCGATAAAAGCAACAAACCCGGTGACAATCCCAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATaa                                                                                                                        <  5:47250/139‑1 (MQ=255)             cgATAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGt                                                                                                                    <  6:447323/139‑1 (MQ=255)              gATAAAAGCAACAAACCCGGGGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGtt                                                                                                                   <  6:223836/139‑1 (MQ=255)               aTAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGAt                                                                                                                          >  7:385688/1‑131 (MQ=255)               aTAAAAGCAACAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGAt                                                                                                                          <  8:385688/131‑1 (MQ=255)                 aaaaGCAACAAACCCGGTGACAATCACAGTAAAAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTccc                                                                                                                <  3:468/139‑1 (MQ=255)                        aCAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  4:264502/116‑1 (MQ=255)                        aCAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                >  3:264502/1‑116 (MQ=255)                        aCAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  7:112141/116‑1 (MQ=255)                        aCAAACCCGGTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                >  8:112141/1‑116 (MQ=255)                            acccggTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCgag                                                                                                     <  3:215465/134‑1 (MQ=255)                            acccggTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCgag                                                                                                     <  4:139197/134‑1 (MQ=255)                                 gTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATGAGCTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAgag                                                                                                <  4:278938/139‑3 (MQ=255)                                 gTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAgag                                                                                                <  3:186904/139‑3 (MQ=255)                                 gTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAgag                                                                                                >  5:257197/1‑137 (MQ=255)                                 gTGACAATCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAgag                                                                                                >  4:46904/1‑137 (MQ=255)                                      aaTCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGata                                                                                           >  4:215308/1‑139 (MQ=255)                                        tCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATCTGGAACCACTTTTATCGTCCATTACTCTACATTGCTAATCAAATACACCGCAAGAAGGACAGGCATAACAGTTCCCTGGTAGACGAGAAGAGCGatat                                                                                          >  6:178479/1‑138 (MQ=255)                                              tAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATcc                                                                                   >  1:196776/1‑139 (MQ=255)                                                aCAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCg                                                                                  >  5:73901/1‑138 (MQ=255)                                                    atggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGaa                                                                                >  4:30989/3‑136 (MQ=255)                                                     tggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                            <  5:151524/138‑1 (MQ=255)                                                     tggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                            <  1:119174/138‑1 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  5:112388/84‑1 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                >  6:112388/1‑84 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATg                                                                          >  3:191426/1‑138 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATg                                                                          >  8:46385/1‑138 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGc                                                                         >  4:167349/1‑139 (MQ=255)                                                        tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGc                                                                         <  2:215886/139‑1 (MQ=255)                                                            aCAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCCCATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCatg                                                                      >  8:300294/1‑137 (MQ=255)                                                                gCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAgg                                                                                                                             >  1:327517/1‑79 (MQ=255)                                                                gCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAgg                                                                                                                             <  2:327517/79‑1 (MQ=255)                                                                 cAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAAt                                                                  >  5:122941/1‑137 (MQ=255)                                                                    aTCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCgag                                                                                                     <  7:196519/99‑1 (MQ=255)                                                                    aTCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCgag                                                                                                     >  8:196519/1‑99 (MQ=255)                                                                     tccaCCGCATGTGGAACCACTTTTCTCGTCCAATTCTCTACCTTGCTCATCACATACAGCTCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAATAGCTATATCAAATCCGAAAAGATGCATGTAATCAggtg                                                            <  1:323082/136‑3 (MQ=255)                                                                        aCCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGt                                                                                                                    <  4:33308/80‑1 (MQ=255)                                                                        aCCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGt                                                                                                                    >  3:33308/1‑80 (MQ=255)                                                                            cATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                <  4:45613/64‑1 (MQ=255)                                                                            cATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGAc                                                                                                                                >  3:45613/1‑64 (MQ=255)                                                                            cATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTc                                                     >  4:78276/1‑139 (MQ=255)                                                                                  gAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCaa                                               >  8:14238/1‑139 (MQ=255)                                                                                        ctTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCa                                         >  3:171695/1‑139 (MQ=255)                                                                                          tttCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        <  8:414114/138‑1 (MQ=255)                                                                                           ttCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        <  4:171695/137‑1 (MQ=255)                                                                                                        ctctACCTTGCTCATCAAATACACCGCAAGCAGGAAAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCAGAAACGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAgatt                          <  5:178479/138‑4 (MQ=255)                                                                                                             ccTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGa                      <  1:54248/137‑1 (MQ=255)                                                                                                               ttGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        <  8:186190/117‑1 (MQ=255)                                                                                                               ttGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        >  7:186190/1‑117 (MQ=255)                                                                                                                tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                            >  8:73544/1‑80 (MQ=255)                                                                                                                tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                            <  7:73544/80‑1 (MQ=255)                                                                                                                tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        <  8:72099/116‑1 (MQ=255)                                                                                                                tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                        >  7:72099/1‑116 (MQ=255)                                                                                                                tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGACAATCCCCATGGATTTAAAAGATTCGGATGAAt                 >  2:186769/1‑139 (MQ=255)                                                                                                                  cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGTTGTCGCTTCCGCCAATCCCAATGGATTTAAAAGATTCGGa                      >  6:34364/1‑132 (MQ=255)                                                                                                                  cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCa                                                <  2:237447/106‑1 (MQ=255)                                                                                                                  cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCa                                                >  1:237447/1‑106 (MQ=255)                                                                                                                  cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGa                      <  5:34364/132‑1 (MQ=255)                                                                                                                     atcaCATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGa            <  6:257197/139‑1 (MQ=255)                                                                                                                       cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt          <  8:151440/139‑2 (MQ=255)                                                                                                                       cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt          <  8:51180/139‑2 (MQ=255)                                                                                                                       cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt          <  4:191426/139‑2 (MQ=255)                                                                                                                       cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt          >  2:16240/1‑138 (MQ=255)                                                                                                                        acaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt          <  3:78276/138‑2 (MQ=255)                                                                                                                         caTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTcc        <  6:122941/139‑1 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAAt                 <  1:402396/124‑1 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAAt                 >  2:402396/1‑124 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAttc         >  2:354784/1‑130 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAttc         <  1:354784/132‑3 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGa   >  2:159049/1‑138 (MQ=255)                                                                                                                               ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGAt  >  2:28877/1‑139 (MQ=255)                                                                                                                                     |                                                                                                                                     AAGGACCAATCGCGATAAAAGCGACAAATCCAGTGACAATCACTGTAACAAAAGGTGTAACAAGCAAATCTACCGCATGCGGAACTACTTTTCTTGTCCATTTCTCTACCTTGCTCATCACATAAACCGCCAACAAGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGAT  >  CP006881/3722079‑3722343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑