Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006881	3,722,248	C→A	A213A (GCG→GCT)	U712_19190 ←	PTS system sucrose‑specific EIIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,722,248	0	C	A	100.0%	280.5 / NA	90	A213A (GCG→GCT)	U712_19190	PTS system sucrose‑specific EIIBC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (53/37);  total (53/37)

AATCACTGTAACAAAAGGTGTAACAAGCAAATCTACCGCATGCGGAACTACTTTTCTTGTCCATTTCTCTACCTTGCTCATCACATAAACCGCCAACAAGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGA  >  CP006881/3722116‑3722382                                                                                                                                     |                                                                                                                                       aaTCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGata                                                                                                                                  >  4:215308/1‑139 (MQ=255)   tCACAGTAACAAATGGTGTAACAAGCAAATCCACCGCATCTGGAACCACTTTTATCGTCCATTACTCTACATTGCTAATCAAATACACCGCAAGAAGGACAGGCATAACAGTTCCCTGGTAGACGAGAAGAGCGatat                                                                                                                                 >  6:178479/1‑138 (MQ=255)         tAACAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATcc                                                                                                                          >  1:196776/1‑139 (MQ=255)           aCAAATGGTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCg                                                                                                                         >  5:73901/1‑138 (MQ=255)               atggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGaa                                                                                                                       >  4:30989/3‑136 (MQ=255)                tggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   <  1:119174/138‑1 (MQ=255)                tggTGTAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   <  5:151524/138‑1 (MQ=255)                   tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATg                                                                                                                 >  8:46385/1‑138 (MQ=255)                   tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATg                                                                                                                 >  3:191426/1‑138 (MQ=255)                   tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGc                                                                                                                <  2:215886/139‑1 (MQ=255)                   tgtAACAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGc                                                                                                                >  4:167349/1‑139 (MQ=255)                       aCAAGCAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCCCATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCatg                                                                                                             >  8:300294/1‑137 (MQ=255)                            cAAATCCACCGCATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAAt                                                                                                         >  5:122941/1‑137 (MQ=255)                                tccaCCGCATGTGGAACCACTTTTCTCGTCCAATTCTCTACCTTGCTCATCACATACAGCTCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAATAGCTATATCAAATCCGAAAAGATGCATGTAATCAggtg                                                                                                   <  1:323082/136‑3 (MQ=255)                                       cATGTGGAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTc                                                                                            >  4:78276/1‑139 (MQ=255)                                             gAACCACTTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCaa                                                                                      >  8:14238/1‑139 (MQ=255)                                                   ctTTTCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCa                                                                                >  3:171695/1‑139 (MQ=255)                                                     tttCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               <  8:414114/138‑1 (MQ=255)                                                      ttCTCGTCCATTTCTCTACCTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               <  4:171695/137‑1 (MQ=255)                                                                   ctctACCTTGCTCATCAAATACACCGCAAGCAGGAAAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCAGAAACGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAgatt                                                                 <  5:178479/138‑4 (MQ=255)                                                                        ccTTGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGa                                                             <  1:54248/137‑1 (MQ=255)                                                                          ttGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               >  7:186190/1‑117 (MQ=255)                                                                          ttGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               <  8:186190/117‑1 (MQ=255)                                                                           tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   >  8:73544/1‑80 (MQ=255)                                                                           tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   <  7:73544/80‑1 (MQ=255)                                                                           tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               >  7:72099/1‑116 (MQ=255)                                                                           tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               <  8:72099/116‑1 (MQ=255)                                                                           tGCTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGACAATCCCCATGGATTTAAAAGATTCGGATGAAt                                                        >  2:186769/1‑139 (MQ=255)                                                                             cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGTTGTCGCTTCCGCCAATCCCAATGGATTTAAAAGATTCGGa                                                             >  6:34364/1‑132 (MQ=255)                                                                             cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCa                                                                                       >  1:237447/1‑106 (MQ=255)                                                                             cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCa                                                                                       <  2:237447/106‑1 (MQ=255)                                                                             cTCATCACATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGa                                                             <  5:34364/132‑1 (MQ=255)                                                                                atcaCATACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGa                                                   <  6:257197/139‑1 (MQ=255)                                                                                  cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt                                                 <  4:191426/139‑2 (MQ=255)                                                                                  cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt                                                 <  8:51180/139‑2 (MQ=255)                                                                                  cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt                                                 >  2:16240/1‑138 (MQ=255)                                                                                  cacaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt                                                 <  8:151440/139‑2 (MQ=255)                                                                                   acaTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAtt                                                 <  3:78276/138‑2 (MQ=255)                                                                                    caTACACCGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTcc                                               <  6:122941/139‑1 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAAt                                                        <  1:402396/124‑1 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAAt                                                        >  2:402396/1‑124 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAttc                                                <  1:354784/132‑3 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGAttc                                                >  2:354784/1‑130 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGa                                          >  2:159049/1‑138 (MQ=255)                                                                                          ccGCAAGCAGGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGAt                                         >  2:28877/1‑139 (MQ=255)                                                                                               agcaggACAGGGATAACAGTTCCCTGGTATCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATTTAATCAGGTGTCGCTTCCTCCAATCCCCATTGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    <  7:356436/134‑1 (MQ=255)                                                                                               agcaggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    >  5:5789/6‑139 (MQ=255)                                                                                               agcaggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    <  5:15486/134‑1 (MQ=255)                                                                                               agcaggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCC‑AATCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGc                                   >  1:130214/6‑139 (MQ=255)                                                                                               agcaggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGGCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    <  3:46904/134‑1 (MQ=255)                                                                                                 caggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCt                                  <  1:186769/136‑1 (MQ=255)                                                                                                  aggACAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTc                                 <  5:200587/137‑1 (MQ=255)                                                                                                   ggACAGGGATAACAGTTCCCTGGTAGCCGAGAAgagc                                                                                                                                      >  8:378995/2‑37 (MQ=255)                                                                                                   ggACAGGGATAACAGTTCCCTGGTAGCCGAGAAgagc                                                                                                                                      <  7:378995/36‑1 (MQ=255)                                                                                                     aCAGGGATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCACGATGACCGCTccc                               >  4:363653/1‑138 (MQ=255)                                                                                                         ggATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTa                          >  2:291618/1‑139 (MQ=255)                                                                                                         ggATAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATCAATCATGATTCCCCAGATGACCGCTCCCAAGTa                          >  4:251662/1‑139 (MQ=255)                                                                                                            tAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    <  1:19884/126‑1 (MQ=255)                                                                                                            tAACAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCg                                    >  2:19884/1‑126 (MQ=255)                                                                                                              aCAGTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATggat                     >  7:417944/1‑137 (MQ=255)                                                                                                                 gTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGAcc                                     >  4:236911/1‑120 (MQ=255)                                                                                                                 gTTCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGAcc                                     <  3:236911/120‑1 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               >  1:162913/1‑76 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGCTTCCACCGATGACCGCTCCCAAGTATGGATTGCtg                >  8:422295/1‑138 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTcc                                <  6:415129/123‑1 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTcc                                >  5:415129/1‑123 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtg                >  6:174001/1‑138 (MQ=255)                                                                                                                   tCCCTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtg                >  8:85804/1‑138 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   >  2:189591/1‑39 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGa                                                                                                                   <  1:189591/39‑1 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               >  7:193819/1‑75 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGTATGAATCATGATTCCCCCGATGCCCTCTCCCAAGTATGGATTGCtgc               >  3:295781/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATggat                     <  8:419594/133‑3 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATggat                     >  7:419594/1‑131 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  5:211849/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  1:206484/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  7:89982/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  3:258100/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  1:105392/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTACGCCAATCCCCATCGATTTAAAACATACTGATGAATAATGAATCACCCGATGACCGCACCCAAGTATGGATTCCtgc               >  2:226086/1‑137 (MQ=255)                                                                                                                    cccTGGTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAAACAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCtgc               >  1:316134/1‑137 (MQ=255)                                                                                                                       tGGTAGCCGAGAAGAGCGATATCAAAACCGAAAAGATGCATGTATTCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATACGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCa             >  8:191782/1‑138 (MQ=255)                                                                                                                         gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCTTGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa           <  3:167349/138‑1 (MQ=255)                                                                                                                         gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa           <  7:14238/138‑1 (MQ=255)                                                                                                                         gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa           <  4:258100/138‑1 (MQ=255)                                                                                                                         gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa           <  8:89982/138‑1 (MQ=255)                                                                                                                          tAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCAt                                                                               >  7:394282/1‑69 (MQ=255)                                                                                                                          tAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAAct         >  2:115191/1‑139 (MQ=255)                                                                                                                            gCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAActct       >  1:350743/1‑139 (MQ=255)                                                                                                                            gCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAActct       >  6:87904/1‑139 (MQ=255)                                                                                                                                 gaAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGa  <  2:206484/139‑1 (MQ=255)                                                                                                                                 gaAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGa  <  6:211849/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       AATCACTGTAACAAAAGGTGTAACAAGCAAATCTACCGCATGCGGAACTACTTTTCTTGTCCATTTCTCTACCTTGCTCATCACATAAACCGCCAACAAGACAGGGATAACAGTTCCCTGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGA  >  CP006881/3722116‑3722382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑