| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006881 | 3,722,368 | T→G | G173G (GGA→GGC) | U712_19190 ← | PTS system sucrose‑specific EIIBC component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006881 | 3,722,368 | 0 | T | G | 96.8% | 198.5 / ‑3.7 | 63 | G173G (GGA→GGC) | U712_19190 | PTS system sucrose‑specific EIIBC component |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (22/39); total (23/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
TGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAA > CP006881/3722234‑3722502 | tGGTAGCCGAGAAGAGCGATATCAAAACCGAAAAGATGCATGTATTCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATACGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCa > 8:191782/1‑138 (MQ=255) gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCTTGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa < 3:167349/138‑1 (MQ=255) gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa < 8:89982/138‑1 (MQ=255) gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa < 7:14238/138‑1 (MQ=255) gTAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCaaa < 4:258100/138‑1 (MQ=255) tAGCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAAct > 2:115191/1‑139 (MQ=255) gCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAActct > 1:350743/1‑139 (MQ=255) gCCGAGAAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAActct > 6:87904/1‑139 (MQ=255) gaAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGa < 6:211849/139‑1 (MQ=255) gaAGAGCGATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGa < 2:206484/139‑1 (MQ=255) agcgATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGcg < 1:16240/138‑2 (MQ=255) agcgATATCAAATCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGcg > 6:252528/2‑138 (MQ=255) tatCAAATCCGAAAAGATGCATGTAAACAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGac < 2:316134/139‑1 (MQ=255) accAACTCCGAAAAGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGacc < 4:282338/137‑2 (MQ=255) aaaaGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTagaa > 8:17950/4‑139 (MQ=255) aaaaGATGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTagaa > 8:11479/4‑139 (MQ=255) aTGCATGTAATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATAATGATTACCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGAGCTGACCCCGATTAGAATCgg > 7:340136/1‑138 (MQ=255) tGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGc > 7:390839/1‑138 (MQ=255) tgtaATCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGa > 3:341317/3‑138 (MQ=255) taATCAGGTGTCGCTTCCGCC‑AATCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAaaat < 2:130214/139‑1 (MQ=255) aaTCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGCATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGCATTGCTGCCAAACTCTATTGAAGCGCTGACCCCGATTAGAAACGGCAAGAaaat > 3:167851/1‑139 (MQ=255) aTCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAaaat < 5:87904/138‑1 (MQ=255) tCAGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAaaatg > 3:361392/1‑137 (MQ=255) aGGTGTCGCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAaaatgaa < 6:5789/138‑1 (MQ=255) gtgtcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGc > 7:234521/6‑139 (MQ=255) gtgtcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGc > 1:209822/6‑139 (MQ=255) gtcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 2:196776/136‑3 (MQ=255) gtcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 7:11479/136‑3 (MQ=255) gtcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 2:350743/136‑3 (MQ=255) tcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 7:17950/136‑3 (MQ=255) tcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 5:252528/136‑3 (MQ=255) tcgCTTCCGCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGctg < 8:417944/136‑3 (MQ=255) ccGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACtt < 8:390839/137‑1 (MQ=255) gCCAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTgaaa < 3:225532/139‑3 (MQ=255) ccAATCCCCATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGaaaa < 3:30989/139‑1 (MQ=255) aatccccATGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACa < 3:409994/136‑1 (MQ=255) aTGGATTTAAAAGATTCGGATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAag > 7:48640/1‑139 (MQ=255) ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa < 3:419721/138‑1 (MQ=255) ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa < 3:215308/138‑1 (MQ=255) ggATGAATCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCa < 1:291618/138‑1 (MQ=255) aaTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAg < 4:41010/134‑1 (MQ=255) aaTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAg > 3:41010/1‑134 (MQ=255) aTCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCt > 7:149772/1‑139 (MQ=255) tCATGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCtt > 6:386648/1‑139 (MQ=255) aTGATTCCCCCGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAATCAGCTGCAAAAGCGCAGAATCCTTg < 2:414718/138‑1 (MQ=255) attcccccGATGTCCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa < 8:234521/133‑1 (MQ=255) attcccccGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCa < 8:149772/133‑1 (MQ=255) cccccGATGACCGCTCCAAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATc < 7:191782/135‑1 (MQ=255) ccccGATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATcc > 5:385590/3‑138 (MQ=255) gATGACCGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGa < 4:259019/139‑1 (MQ=255) cGCTCCCAATTATGGAGTGCAGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGACTCGGCAAGAAAATGAATGCTGCACTTGAAACCATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa < 5:24192/139‑1 (MQ=255) cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa < 7:46385/139‑1 (MQ=255) cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa < 3:374362/139‑1 (MQ=255) cGCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTCGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCa < 7:85804/139‑1 (MQ=255) gCTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAt < 6:385590/139‑1 (MQ=255) cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGCATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCAtt > 1:415294/1‑139 (MQ=255) cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGc < 7:269154/59‑1 (MQ=255) cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGc > 8:269154/1‑59 (MQ=255) cTCCCAAGTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTCCTCATCCAATGAAACGCAtt > 5:269928/1‑139 (MQ=255) gTATGGATTGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATa < 7:300294/139‑1 (MQ=255) tatggattGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATAc < 1:115191/133‑1 (MQ=255) attGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCag > 5:154838/2‑137 (MQ=255) ttGCTGCCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagca < 2:105392/139‑4 (MQ=255) tgctgcCAAACTCTTTTGAAGCGCTGACCCCGATTAGAATCGGCAAGAAAATGAATGCTGCACTTGAAAACATATCAAGCAGCTGCAAAAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCagcaa > 2:262364/1‑135 (MQ=255) | TGGTAGCCGAGAAGCGCGATATCAAATCCGAACAGATGCATATAATCAGGGGTTGCTTCCGCCAAACCCCATGGATTCAAAAGCGTCGGATGAATCATGATACCTCCGATGACCGCTCCCAAGTAAGGGTTGCTTCCAAACTCTTTTGAAGCACTGACACCGATTAAAATCGGCAAGAAAATAAATGCCGCACTTGAGAACATGTCGAGCAGCTGCAGCAGCGCAGAATCCTTGCTCATCCAATGAAACGCATTGATCATACCCAATAA > CP006881/3722234‑3722502 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |